Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization

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Original languageEnglish
Article number355
JournalJournal of Fungi
Volume8
Issue number4
Early online date30 Mar 2022
Publication statusPublished - Apr 2022

Abstract

Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterol-ogous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.

Keywords

    heterologous expression, microbial cell factory, PKS, PKS-NRPS, Trichoderma reesei, waste valorization

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Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization. / Shenouda, Mary L.; Ambilika, Maria; Skellam, Elizabeth et al.
In: Journal of Fungi, Vol. 8, No. 4, 355, 04.2022.

Research output: Contribution to journalArticleResearchpeer review

Shenouda ML, Ambilika M, Skellam E, Cox RJ. Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization. Journal of Fungi. 2022 Apr;8(4):355. Epub 2022 Mar 30. doi: 10.3390/jof8040355
Shenouda, Mary L. ; Ambilika, Maria ; Skellam, Elizabeth et al. / Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization. In: Journal of Fungi. 2022 ; Vol. 8, No. 4.
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abstract = "Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterol-ogous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.",
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note = "Funding Information: Funding: M.L.S. thanks the German Academic Exchange Service (DAAD) and the Egyptian Ministry of Higher Education and Scientific Research (MHESR) for funding (GERLS programme, 2017, 57311832). This work was supported by DFG (CO 1328/2-1, INST 187/621-1, INST 187/686-1). The APC was funded by the Open Access Fund of the Leibniz Universit{\"a}t Hannover.",
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AU - Shenouda, Mary L.

AU - Ambilika, Maria

AU - Skellam, Elizabeth

AU - Cox, Russell J.

N1 - Funding Information: Funding: M.L.S. thanks the German Academic Exchange Service (DAAD) and the Egyptian Ministry of Higher Education and Scientific Research (MHESR) for funding (GERLS programme, 2017, 57311832). This work was supported by DFG (CO 1328/2-1, INST 187/621-1, INST 187/686-1). The APC was funded by the Open Access Fund of the Leibniz Universität Hannover.

PY - 2022/4

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N2 - Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterol-ogous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.

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