Details
| Original language | English |
|---|---|
| Pages (from-to) | 1107-1118 |
| Number of pages | 12 |
| Journal | Brazilian Journal of Microbiology |
| Volume | 52 |
| Issue number | 3 |
| Early online date | 20 Apr 2021 |
| Publication status | Published - Sept 2021 |
| Externally published | Yes |
Abstract
in their natural hosts. To investigate whether actinorhodin-overproducer Streptomyces coelicolor Δppk mutant strain could be a
possible prototype as a heterologous expression host, a cosmid containing most of the elm gene cluster of Streptomyces olivaceus
Tü2353 was integrated into chromosomes of both S. coelicolor A3(2) and Δppk strains. Interestingly, it was found that the
production of tetracyclic polyketide 8-demethyl-tetracenomycin (8-DMTC) by recombinant strains caused significant changes in
the morphology of cells. All the pellets and clumps were disentangled and mycelia were fragmented in the recombinant strains.
Moreover, they produce neither pigmented antibiotics nor agarase and did not sporulate. By eliminating the elm biosynthesis
genes from the cosmid, we showed that the morphological properties of recombinants were caused by the production of 8-
DMTC. Extracellular application of 8-DMTC on S. coelicolor wild-type cells caused a similar phenotype with the 8-DMTCproducing recombinant strains. The results of this study may contribute to the understanding of the effect of 8-DMTC in
Streptomyces since the morphological changes that we have observed have not been reported before. It is also valuable in that
it provides useful information about the use of Streptomyces as hosts for the heterologous expression of 8-DMTC.
Keywords
- Δppk, Heterologous expression, Streptomyces colony morphology, Pellet disaggregation, 8-DMTC, ∆ppk
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
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In: Brazilian Journal of Microbiology, Vol. 52, No. 3, 09.2021, p. 1107-1118.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Heterologous expression of 8-demethyl-tetracenomycin (8-dmtc) affected Streptomyces coelicolor life cycle
AU - Cinar, Buse
AU - Demir, Zeynep
AU - Tunca, Sedef
N1 - Funding Information: We are grateful to Dr. Jose A. Salas and Dr. Angel Manteca for their critical reading of the manuscript and valuable comments and suggestions. We would like to thank Dr. Jose A. Salas and Dr. Carmen Mendez (Universidad de Oviedo, Spain) for providing us cos16F4ie.
PY - 2021/9
Y1 - 2021/9
N2 - Heterologous hosts are highly important to detect the expression of biosynthetic gene clusters that are cryptic or poorly expressedin their natural hosts. To investigate whether actinorhodin-overproducer Streptomyces coelicolor Δppk mutant strain could be apossible prototype as a heterologous expression host, a cosmid containing most of the elm gene cluster of Streptomyces olivaceusTü2353 was integrated into chromosomes of both S. coelicolor A3(2) and Δppk strains. Interestingly, it was found that theproduction of tetracyclic polyketide 8-demethyl-tetracenomycin (8-DMTC) by recombinant strains caused significant changes inthe morphology of cells. All the pellets and clumps were disentangled and mycelia were fragmented in the recombinant strains.Moreover, they produce neither pigmented antibiotics nor agarase and did not sporulate. By eliminating the elm biosynthesisgenes from the cosmid, we showed that the morphological properties of recombinants were caused by the production of 8-DMTC. Extracellular application of 8-DMTC on S. coelicolor wild-type cells caused a similar phenotype with the 8-DMTCproducing recombinant strains. The results of this study may contribute to the understanding of the effect of 8-DMTC inStreptomyces since the morphological changes that we have observed have not been reported before. It is also valuable in thatit provides useful information about the use of Streptomyces as hosts for the heterologous expression of 8-DMTC.
AB - Heterologous hosts are highly important to detect the expression of biosynthetic gene clusters that are cryptic or poorly expressedin their natural hosts. To investigate whether actinorhodin-overproducer Streptomyces coelicolor Δppk mutant strain could be apossible prototype as a heterologous expression host, a cosmid containing most of the elm gene cluster of Streptomyces olivaceusTü2353 was integrated into chromosomes of both S. coelicolor A3(2) and Δppk strains. Interestingly, it was found that theproduction of tetracyclic polyketide 8-demethyl-tetracenomycin (8-DMTC) by recombinant strains caused significant changes inthe morphology of cells. All the pellets and clumps were disentangled and mycelia were fragmented in the recombinant strains.Moreover, they produce neither pigmented antibiotics nor agarase and did not sporulate. By eliminating the elm biosynthesisgenes from the cosmid, we showed that the morphological properties of recombinants were caused by the production of 8-DMTC. Extracellular application of 8-DMTC on S. coelicolor wild-type cells caused a similar phenotype with the 8-DMTCproducing recombinant strains. The results of this study may contribute to the understanding of the effect of 8-DMTC inStreptomyces since the morphological changes that we have observed have not been reported before. It is also valuable in thatit provides useful information about the use of Streptomyces as hosts for the heterologous expression of 8-DMTC.
KW - Δppk
KW - Heterologous expression
KW - Streptomyces colony morphology
KW - Pellet disaggregation
KW - 8-DMTC
KW - ∆ppk
UR - http://www.scopus.com/inward/record.url?scp=85111926202&partnerID=8YFLogxK
U2 - 10.1007/s42770-021-00499-y
DO - 10.1007/s42770-021-00499-y
M3 - Article
VL - 52
SP - 1107
EP - 1118
JO - Brazilian Journal of Microbiology
JF - Brazilian Journal of Microbiology
SN - 1517-8382
IS - 3
ER -