Details
| Original language | English |
|---|---|
| Pages (from-to) | 3532-3541 |
| Number of pages | 10 |
| Journal | BIOMACROMOLECULES |
| Volume | 14 |
| Issue number | 10 |
| Publication status | Published - 29 Aug 2013 |
Abstract
Single-walled carbon nanotubes (SWNTs) are unique nanostructures used as cargo systems for variety of diagnostic and therapeutic agents. For taking advantage of these structures in biological processes, they should be visible. Therefore, fluorescence labeling of SWCNTs with various probes is a significant issue. Herein, we demonstrate a simple approach for cell specific imaging and diagnosis by combining SWCNTs with a copolymer poly(para-phenylene) (PPP) containing polystyrene (PSt) and poly(ε-caprolactone) (PCL) side chains (PPP-g-PSt-PCL). In this approach PPP-g-PSt-PCL is noncovalently attached on carboxyl functional SWCNTs. The obtained fluorescent probe is bound to folic acid (FA) for targeted imaging of folate receptor (FR) positive HeLa cells. In vitro studies demonstrate that this conjugate can specifically bind to HeLa cells and indicate great potential for targeting and imaging studies.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Materials Science(all)
- Biomaterials
- Materials Science(all)
- Polymers and Plastics
- Materials Science(all)
- Materials Chemistry
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In: BIOMACROMOLECULES, Vol. 14, No. 10, 29.08.2013, p. 3532-3541.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - From Invisible Structures of SWCNTs toward Fluorescent and Targeting Architectures for Cell Imaging
AU - Ag, Didem
AU - Seleci, Muharrem
AU - Bongartz, Rebecca
AU - Can, Mustafa
AU - Yurteri, Seda
AU - Cianga, Ioan
AU - Stahl, Frank
AU - Timur, Suna
AU - Scheper, Thomas
AU - Yagci, Yusuf
PY - 2013/8/29
Y1 - 2013/8/29
N2 - Single-walled carbon nanotubes (SWNTs) are unique nanostructures used as cargo systems for variety of diagnostic and therapeutic agents. For taking advantage of these structures in biological processes, they should be visible. Therefore, fluorescence labeling of SWCNTs with various probes is a significant issue. Herein, we demonstrate a simple approach for cell specific imaging and diagnosis by combining SWCNTs with a copolymer poly(para-phenylene) (PPP) containing polystyrene (PSt) and poly(ε-caprolactone) (PCL) side chains (PPP-g-PSt-PCL). In this approach PPP-g-PSt-PCL is noncovalently attached on carboxyl functional SWCNTs. The obtained fluorescent probe is bound to folic acid (FA) for targeted imaging of folate receptor (FR) positive HeLa cells. In vitro studies demonstrate that this conjugate can specifically bind to HeLa cells and indicate great potential for targeting and imaging studies.
AB - Single-walled carbon nanotubes (SWNTs) are unique nanostructures used as cargo systems for variety of diagnostic and therapeutic agents. For taking advantage of these structures in biological processes, they should be visible. Therefore, fluorescence labeling of SWCNTs with various probes is a significant issue. Herein, we demonstrate a simple approach for cell specific imaging and diagnosis by combining SWCNTs with a copolymer poly(para-phenylene) (PPP) containing polystyrene (PSt) and poly(ε-caprolactone) (PCL) side chains (PPP-g-PSt-PCL). In this approach PPP-g-PSt-PCL is noncovalently attached on carboxyl functional SWCNTs. The obtained fluorescent probe is bound to folic acid (FA) for targeted imaging of folate receptor (FR) positive HeLa cells. In vitro studies demonstrate that this conjugate can specifically bind to HeLa cells and indicate great potential for targeting and imaging studies.
UR - http://www.scopus.com/inward/record.url?scp=84885625530&partnerID=8YFLogxK
U2 - 10.1021/bm400862m
DO - 10.1021/bm400862m
M3 - Article
C2 - 23987303
AN - SCOPUS:84885625530
VL - 14
SP - 3532
EP - 3541
JO - BIOMACROMOLECULES
JF - BIOMACROMOLECULES
SN - 1525-7797
IS - 10
ER -