Details
| Original language | English |
|---|---|
| Pages (from-to) | 139-148 |
| Number of pages | 10 |
| Journal | Journal of biotechnology |
| Volume | 5 |
| Issue number | 2 |
| Publication status | Published - Apr 1987 |
Abstract
A laser flow cytometer was used to study different Escherichia coli populations under various cultivation conditions. A host strain E. coli 5K was analyzed for cell size, protein and DNA-content during continuous cultivation. Also, a recombinant E. coli 5K(pHM12) strain (used for the intracellular production of penicillin-G acylase) was studied in regard to gene expression using different cytometric techniques. An argon ion laser (30 mW) and a 100 W high-pressure mercury lamp were used as light source in the cytometer. A new fluorogenic staining technique for intracellular penicillin-G acylase is described. Recombinant E. coli temperature sensitive cells were analyzed for intracellular fusion protein production due to temperature induction.
Keywords
- Cultivation monitoring, Escherichia coli, Intracellular enzyme activity, Laser flow cytometry
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 5, No. 2, 04.1987, p. 139-148.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Flow cytometry of Escherichia coli for process monitoring
AU - Scheper, T.
AU - Hitzmann, B.
AU - Rinas, U.
AU - Schügerl, K.
PY - 1987/4
Y1 - 1987/4
N2 - A laser flow cytometer was used to study different Escherichia coli populations under various cultivation conditions. A host strain E. coli 5K was analyzed for cell size, protein and DNA-content during continuous cultivation. Also, a recombinant E. coli 5K(pHM12) strain (used for the intracellular production of penicillin-G acylase) was studied in regard to gene expression using different cytometric techniques. An argon ion laser (30 mW) and a 100 W high-pressure mercury lamp were used as light source in the cytometer. A new fluorogenic staining technique for intracellular penicillin-G acylase is described. Recombinant E. coli temperature sensitive cells were analyzed for intracellular fusion protein production due to temperature induction.
AB - A laser flow cytometer was used to study different Escherichia coli populations under various cultivation conditions. A host strain E. coli 5K was analyzed for cell size, protein and DNA-content during continuous cultivation. Also, a recombinant E. coli 5K(pHM12) strain (used for the intracellular production of penicillin-G acylase) was studied in regard to gene expression using different cytometric techniques. An argon ion laser (30 mW) and a 100 W high-pressure mercury lamp were used as light source in the cytometer. A new fluorogenic staining technique for intracellular penicillin-G acylase is described. Recombinant E. coli temperature sensitive cells were analyzed for intracellular fusion protein production due to temperature induction.
KW - Cultivation monitoring
KW - Escherichia coli
KW - Intracellular enzyme activity
KW - Laser flow cytometry
UR - http://www.scopus.com/inward/record.url?scp=0023218136&partnerID=8YFLogxK
U2 - 10.1016/0168-1656(87)90010-1
DO - 10.1016/0168-1656(87)90010-1
M3 - Article
AN - SCOPUS:0023218136
VL - 5
SP - 139
EP - 148
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 2
ER -