Details
| Original language | English |
|---|---|
| Pages (from-to) | 891-904 |
| Number of pages | 14 |
| Journal | Journal of proteome research |
| Volume | 23 |
| Issue number | 3 |
| Early online date | 20 Feb 2024 |
| Publication status | Published - Mar 2024 |
Abstract
Quickly identifying and characterizing isolates from extreme environments is currently challenging while very important to explore the Earth′s biodiversity. As these isolates may, in principle, be distantly related to known species, techniques are needed to reliably identify the branch of life to which they belong. Proteotyping these environmental isolates by tandem mass spectrometry offers a rapid and cost-effective option for their identification using their peptide profiles. In this study, we document the first high-throughput proteotyping approach for environmental extremophilic and halophilic isolates. Microorganisms were isolated from samples originating from high-altitude Andean lakes (3700-4300 m a.s.l.) in the Chilean Altiplano, which represent environments on Earth that resemble conditions on other planets. A total of 66 microorganisms were cultivated and identified by proteotyping and 16S rRNA gene amplicon sequencing. Both the approaches revealed the same genus identification for all isolates except for three isolates possibly representing not yet taxonomically characterized organisms based on their peptidomes. Proteotyping was able to indicate the presence of two potentially new genera from the families of Paracoccaceae and Chromatiaceae/Alteromonadaceae, which have been overlooked by 16S rRNA amplicon sequencing approach only. The paper highlights that proteotyping has the potential to discover undescribed microorganisms from extreme environments.
Keywords
- Altiplano, Atacama Desert, extremophiles, halophiles, high-altitude Andean lakes, tandem mass spectrometry proteotyping
ASJC Scopus subject areas
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
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In: Journal of proteome research, Vol. 23, No. 3, 03.2024, p. 891-904.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Exploring Andean High-Altitude Lake Extremophiles through Advanced Proteotyping
AU - Runzheimer, Katharina
AU - Lozano, Clément
AU - Boy, Diana
AU - Boy, Jens
AU - Godoy, Roberto
AU - Matus, Francisco J.
AU - Engel, Denise
AU - Pavletic, Bruno
AU - Leuko, Stefan
AU - Armengaud, Jean
AU - Moeller, Ralf
N1 - Funding Information: K.R. and R.M. were supported by the BMBF-VDI/VDE Innovation + Technik grant “MultiKulti“ (Förderkennzeichen: 161L0285D). K.R., D.E., S.L., B.P., and R.M. were supported by the German Aerospace Center (Deutsches Zentrum für Luft- und Raumfahrt, DLR) grant FuE-Project “ISS LIFE”, RF-FuW, TP 475). C.L. and J.A. acknowledge the Région Occitanie (grant 21023526-DeepMicro) for support in state-of-the-art tandem mass spectrometry equipment. D.B. and J.B. acknowledge the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG SPP 1803, EarthShape) and the Volkswagen Foundation (FUNCOPE).
PY - 2024/3
Y1 - 2024/3
N2 - Quickly identifying and characterizing isolates from extreme environments is currently challenging while very important to explore the Earth′s biodiversity. As these isolates may, in principle, be distantly related to known species, techniques are needed to reliably identify the branch of life to which they belong. Proteotyping these environmental isolates by tandem mass spectrometry offers a rapid and cost-effective option for their identification using their peptide profiles. In this study, we document the first high-throughput proteotyping approach for environmental extremophilic and halophilic isolates. Microorganisms were isolated from samples originating from high-altitude Andean lakes (3700-4300 m a.s.l.) in the Chilean Altiplano, which represent environments on Earth that resemble conditions on other planets. A total of 66 microorganisms were cultivated and identified by proteotyping and 16S rRNA gene amplicon sequencing. Both the approaches revealed the same genus identification for all isolates except for three isolates possibly representing not yet taxonomically characterized organisms based on their peptidomes. Proteotyping was able to indicate the presence of two potentially new genera from the families of Paracoccaceae and Chromatiaceae/Alteromonadaceae, which have been overlooked by 16S rRNA amplicon sequencing approach only. The paper highlights that proteotyping has the potential to discover undescribed microorganisms from extreme environments.
AB - Quickly identifying and characterizing isolates from extreme environments is currently challenging while very important to explore the Earth′s biodiversity. As these isolates may, in principle, be distantly related to known species, techniques are needed to reliably identify the branch of life to which they belong. Proteotyping these environmental isolates by tandem mass spectrometry offers a rapid and cost-effective option for their identification using their peptide profiles. In this study, we document the first high-throughput proteotyping approach for environmental extremophilic and halophilic isolates. Microorganisms were isolated from samples originating from high-altitude Andean lakes (3700-4300 m a.s.l.) in the Chilean Altiplano, which represent environments on Earth that resemble conditions on other planets. A total of 66 microorganisms were cultivated and identified by proteotyping and 16S rRNA gene amplicon sequencing. Both the approaches revealed the same genus identification for all isolates except for three isolates possibly representing not yet taxonomically characterized organisms based on their peptidomes. Proteotyping was able to indicate the presence of two potentially new genera from the families of Paracoccaceae and Chromatiaceae/Alteromonadaceae, which have been overlooked by 16S rRNA amplicon sequencing approach only. The paper highlights that proteotyping has the potential to discover undescribed microorganisms from extreme environments.
KW - Altiplano
KW - Atacama Desert
KW - extremophiles
KW - halophiles
KW - high-altitude Andean lakes
KW - tandem mass spectrometry proteotyping
UR - http://www.scopus.com/inward/record.url?scp=85186090362&partnerID=8YFLogxK
U2 - 10.1021/acs.jproteome.3c00538
DO - 10.1021/acs.jproteome.3c00538
M3 - Article
C2 - 38377575
AN - SCOPUS:85186090362
VL - 23
SP - 891
EP - 904
JO - Journal of proteome research
JF - Journal of proteome research
SN - 1535-3893
IS - 3
ER -