Differential expression of the PAL gene family in rice seedlings exposed to chromium by microarray analysis

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Xiao Zhang Yu
  • Wei Jia Fan
  • Yu Juan Lin
  • Fei Fei Zhang
  • Dharmendra K. Gupta

External Research Organisations

  • Guilin University of Technology
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Details

Original languageEnglish
Pages (from-to)325-335
Number of pages11
JournalEcotoxicology
Volume27
Issue number3
Early online date5 Feb 2018
Publication statusPublished - 1 Apr 2018

Abstract

Phenylalanine ammonia-lyase (PAL) is one of the principle enzymes involved in plant’s secondary metabolism. Expression of individual isogene from the PAL gene family is variable with species of plants in responses to different stresses. In this study, transcriptome analysis of the PAL gene family in rice seedlings exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) was conducted using Agilent 44K rice microarray and real-time quantitative RT-PCR. Uptake and accumulation of both Cr species by rice seedlings and their effect on PAL activity were also determined. Three days of Cr exposure led to significant accumulation of Cr in plant tissues, but majority being in roots rather than shoots. Changes of PAL activities in rice tissues were evident from both Cr treatments. Individual isogene from the rice PAL gene family was expressed differentially in response to both Cr variants. Comparing gene expression between two Cr treatments, only osPAL2 and osPAL4 genes were expressed in similar patterns. Also, gene expression pattern was inconsistent in both plant tissues. Results indicated that expression of individual isoform from the rice PAL gene family is tissue, and stimulus specific under different Cr exposure, suggesting their different detoxification strategies for decreasing or eliminating Cr stresses.

Keywords

    Chromium, Microarray, Phenylalanine ammonia-lyase, Rice

ASJC Scopus subject areas

Cite this

Differential expression of the PAL gene family in rice seedlings exposed to chromium by microarray analysis. / Yu, Xiao Zhang; Fan, Wei Jia; Lin, Yu Juan et al.
In: Ecotoxicology, Vol. 27, No. 3, 01.04.2018, p. 325-335.

Research output: Contribution to journalArticleResearchpeer review

Yu XZ, Fan WJ, Lin YJ, Zhang FF, Gupta DK. Differential expression of the PAL gene family in rice seedlings exposed to chromium by microarray analysis. Ecotoxicology. 2018 Apr 1;27(3):325-335. Epub 2018 Feb 5. doi: 10.1007/s10646-018-1897-5
Yu, Xiao Zhang ; Fan, Wei Jia ; Lin, Yu Juan et al. / Differential expression of the PAL gene family in rice seedlings exposed to chromium by microarray analysis. In: Ecotoxicology. 2018 ; Vol. 27, No. 3. pp. 325-335.
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title = "Differential expression of the PAL gene family in rice seedlings exposed to chromium by microarray analysis",
abstract = "Phenylalanine ammonia-lyase (PAL) is one of the principle enzymes involved in plant{\textquoteright}s secondary metabolism. Expression of individual isogene from the PAL gene family is variable with species of plants in responses to different stresses. In this study, transcriptome analysis of the PAL gene family in rice seedlings exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) was conducted using Agilent 44K rice microarray and real-time quantitative RT-PCR. Uptake and accumulation of both Cr species by rice seedlings and their effect on PAL activity were also determined. Three days of Cr exposure led to significant accumulation of Cr in plant tissues, but majority being in roots rather than shoots. Changes of PAL activities in rice tissues were evident from both Cr treatments. Individual isogene from the rice PAL gene family was expressed differentially in response to both Cr variants. Comparing gene expression between two Cr treatments, only osPAL2 and osPAL4 genes were expressed in similar patterns. Also, gene expression pattern was inconsistent in both plant tissues. Results indicated that expression of individual isoform from the rice PAL gene family is tissue, and stimulus specific under different Cr exposure, suggesting their different detoxification strategies for decreasing or eliminating Cr stresses.",
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AU - Zhang, Fei Fei

AU - Gupta, Dharmendra K.

N1 - Funding information: This work was financially supported by the National Natural Science Foundation of China (No. 41761094) and The Guangxi Talent Highland for Hazardous Waste Disposal Industrialization. X-Z Yu has received the grants from the National Natural Science Foundation of China. The remaining authors declare that they have no conflict of interest. Conflict of interest X-Z Yu has received the grants from the National Natural Science Foundation of China. The remaining authors declare that they have no conflict of interest. Acknowledgements This work was financially supported by the National Natural Science Foundation of China (No. 41761094) and The Guangxi Talent Highland for Hazardous Waste Disposal Industrialization.

PY - 2018/4/1

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N2 - Phenylalanine ammonia-lyase (PAL) is one of the principle enzymes involved in plant’s secondary metabolism. Expression of individual isogene from the PAL gene family is variable with species of plants in responses to different stresses. In this study, transcriptome analysis of the PAL gene family in rice seedlings exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) was conducted using Agilent 44K rice microarray and real-time quantitative RT-PCR. Uptake and accumulation of both Cr species by rice seedlings and their effect on PAL activity were also determined. Three days of Cr exposure led to significant accumulation of Cr in plant tissues, but majority being in roots rather than shoots. Changes of PAL activities in rice tissues were evident from both Cr treatments. Individual isogene from the rice PAL gene family was expressed differentially in response to both Cr variants. Comparing gene expression between two Cr treatments, only osPAL2 and osPAL4 genes were expressed in similar patterns. Also, gene expression pattern was inconsistent in both plant tissues. Results indicated that expression of individual isoform from the rice PAL gene family is tissue, and stimulus specific under different Cr exposure, suggesting their different detoxification strategies for decreasing or eliminating Cr stresses.

AB - Phenylalanine ammonia-lyase (PAL) is one of the principle enzymes involved in plant’s secondary metabolism. Expression of individual isogene from the PAL gene family is variable with species of plants in responses to different stresses. In this study, transcriptome analysis of the PAL gene family in rice seedlings exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) was conducted using Agilent 44K rice microarray and real-time quantitative RT-PCR. Uptake and accumulation of both Cr species by rice seedlings and their effect on PAL activity were also determined. Three days of Cr exposure led to significant accumulation of Cr in plant tissues, but majority being in roots rather than shoots. Changes of PAL activities in rice tissues were evident from both Cr treatments. Individual isogene from the rice PAL gene family was expressed differentially in response to both Cr variants. Comparing gene expression between two Cr treatments, only osPAL2 and osPAL4 genes were expressed in similar patterns. Also, gene expression pattern was inconsistent in both plant tissues. Results indicated that expression of individual isoform from the rice PAL gene family is tissue, and stimulus specific under different Cr exposure, suggesting their different detoxification strategies for decreasing or eliminating Cr stresses.

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