Details
| Original language | English |
|---|---|
| Pages (from-to) | 1792-1806 |
| Number of pages | 15 |
| Journal | LAB on a chip |
| Volume | 20 |
| Issue number | 10 |
| Publication status | Published - 16 Apr 2020 |
Abstract
Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models forin vitrostudy. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent, and biocompatible. Two-photon polymerisation (2PP) enables precise microfabrication of three-dimensional structures. In this study, we report the identification of two biomaterials that support the growth and differentiation of human iPSC-derived neural progenitors into functional neuronal networks. Furthermore, these materials can be patterned to induce alignment of neuronal processes and enable the optical interrogation of individual cells. 2PP scaffolds with tailored topographies therefore provide an effective method of producing definedin vitrohuman neural networks for application in influencing neurite guidance and complex network activity.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- General Chemistry
- Engineering(all)
- Biomedical Engineering
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In: LAB on a chip, Vol. 20, No. 10, 16.04.2020, p. 1792-1806.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
AU - Crowe, J. A.
AU - El-Tamer, A.
AU - Nagel, D.
AU - Koroleva, A. V.
AU - Madrid-Wolff, J.
AU - Olarte, O. E.
AU - Sokolovsky, S.
AU - Estevez-Priego, E.
AU - Ludl, A.
AU - Soriano, J.
AU - Loza-Alvarez, P.
AU - Chichkov, B. N.
AU - Hill, E. J.
AU - Parri, H. R.
AU - Rafailov, E. U.
N1 - Funding information: [ This project has received funding from the European Union's Horizon 2020 research and innovation programme under the grant agreement MESOBRAIN No 713140. JS and EE acknowledge financial support from the Spanish Ministerio de Economia y Competitividad through projects no. FIS2013-41144-P, FIS2016-78507-C2-2-P and FIS2017-90782-REDT (IBERSINC), and from the Generalitat de Catalunya through grant no. 2017- SGR-1061. PL-A, OO and JM-W acknowledge financial support from the Spanish Ministerio de Economia y Competitividad through the “Severo Ochoa” program for Centres of Excellence in R&D (SEV-2015-0522, FIS2016-80455-R; AEI/FEDER, UE), Fundació Privada Cellex, Fundacio'n Mig-Puig and from Generalitat de Catalunya through the “CERCA program” and EU H2020 LaserLab Europe grant 654148. EUR and BNC acknowledge further financial support from the European Union's Horizon 2020 programme under the grant agreement SCAFFOLD-NEEDS No. 851734. We thank Charlie Clark-Bland, Aston University, for assistance with confocal imaging. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the grant agreement MESOBRAIN No 713140. JS and EE acknowledge financial support from the Spanish Ministerio de Economia y Competitividad through projects no. FIS2013-41144-P, FIS2016-78507-C2-2-P and FIS2017-90782-REDT (IBERSINC), and from the Generalitat de Catalunya through grant no. 2017-SGR-1061. PL-A, OO and JM-W acknowledge financial support from the Spanish Ministerio de Economia y Competitividad through the ?Severo Ochoa? program for Centres of Excellence in R&D (SEV-2015-0522, FIS2016-80455-R; AEI/FEDER, UE), Fundaci? Privada Cellex, Fundacio'n Mig-Puig and from Generalitat de Catalunya through the ?CERCA program? and EU H2020 LaserLab Europe grant 654148. EUR and BNC acknowledge further financial support from the European Union's Horizon 2020 programme under the grant agreement SCAFFOLD-NEEDS No. 851734. We thank Charlie Clark-Bland, Aston University, for assistance with confocal imaging.
PY - 2020/4/16
Y1 - 2020/4/16
N2 - Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models forin vitrostudy. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent, and biocompatible. Two-photon polymerisation (2PP) enables precise microfabrication of three-dimensional structures. In this study, we report the identification of two biomaterials that support the growth and differentiation of human iPSC-derived neural progenitors into functional neuronal networks. Furthermore, these materials can be patterned to induce alignment of neuronal processes and enable the optical interrogation of individual cells. 2PP scaffolds with tailored topographies therefore provide an effective method of producing definedin vitrohuman neural networks for application in influencing neurite guidance and complex network activity.
AB - Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models forin vitrostudy. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent, and biocompatible. Two-photon polymerisation (2PP) enables precise microfabrication of three-dimensional structures. In this study, we report the identification of two biomaterials that support the growth and differentiation of human iPSC-derived neural progenitors into functional neuronal networks. Furthermore, these materials can be patterned to induce alignment of neuronal processes and enable the optical interrogation of individual cells. 2PP scaffolds with tailored topographies therefore provide an effective method of producing definedin vitrohuman neural networks for application in influencing neurite guidance and complex network activity.
UR - http://www.scopus.com/inward/record.url?scp=85084943122&partnerID=8YFLogxK
U2 - 10.1039/c9lc01209e
DO - 10.1039/c9lc01209e
M3 - Article
C2 - 32314760
AN - SCOPUS:85084943122
VL - 20
SP - 1792
EP - 1806
JO - LAB on a chip
JF - LAB on a chip
SN - 1473-0197
IS - 10
ER -