Details
| Original language | English |
|---|---|
| Pages (from-to) | 263-268 |
| Number of pages | 6 |
| Journal | Engineering in life sciences |
| Volume | 18 |
| Issue number | 4 |
| Publication status | Published - Apr 2018 |
| Externally published | Yes |
Abstract
In this work, an approach for SPR spectroscopy using the liSPR system is examined that combines signal amplification by PCR and magnetic nanoparticles in one injection step. Therefore, the synthesis of PCR products was performed on the beads similar to a solid-phase PCR, termed PCR-on-a-bead. The functionality of this PCR was proven using an enzymatic assay. For validation the detection of oligonucleotides by SPR, an asymmetric PCR product was investigated. A signal increase upon binding of the PCR product to the specific probes was observed. In addition, surface regeneration of the chip was examined and reuse for at least two times ascertained. Amplification of the SPR signal by magnetic beads was verified but no signal was detected for PCR products immobilized on particles prior to injection.
Keywords
- Magnetic nanoparticles, Signal amplification, Solid phase PCR, Staphylococcus aureus, Surface plasmon resonance
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Environmental Science(all)
- Environmental Engineering
- Chemical Engineering(all)
- Bioengineering
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In: Engineering in life sciences, Vol. 18, No. 4, 04.2018, p. 263-268.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Detection and identification of Staphylococcus aureus using magnetic particle enhanced surface plasmon spectroscopy
AU - Rüppel, Nadine
AU - Tröger, Vicky
AU - Sandetskaya, Natalia
AU - Kuhlmeier, Dirk
AU - Schmieder, Stefan
AU - Sonntag, Frank
N1 - Publisher Copyright: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/4
Y1 - 2018/4
N2 - In this work, an approach for SPR spectroscopy using the liSPR system is examined that combines signal amplification by PCR and magnetic nanoparticles in one injection step. Therefore, the synthesis of PCR products was performed on the beads similar to a solid-phase PCR, termed PCR-on-a-bead. The functionality of this PCR was proven using an enzymatic assay. For validation the detection of oligonucleotides by SPR, an asymmetric PCR product was investigated. A signal increase upon binding of the PCR product to the specific probes was observed. In addition, surface regeneration of the chip was examined and reuse for at least two times ascertained. Amplification of the SPR signal by magnetic beads was verified but no signal was detected for PCR products immobilized on particles prior to injection.
AB - In this work, an approach for SPR spectroscopy using the liSPR system is examined that combines signal amplification by PCR and magnetic nanoparticles in one injection step. Therefore, the synthesis of PCR products was performed on the beads similar to a solid-phase PCR, termed PCR-on-a-bead. The functionality of this PCR was proven using an enzymatic assay. For validation the detection of oligonucleotides by SPR, an asymmetric PCR product was investigated. A signal increase upon binding of the PCR product to the specific probes was observed. In addition, surface regeneration of the chip was examined and reuse for at least two times ascertained. Amplification of the SPR signal by magnetic beads was verified but no signal was detected for PCR products immobilized on particles prior to injection.
KW - Magnetic nanoparticles
KW - Signal amplification
KW - Solid phase PCR
KW - Staphylococcus aureus
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=85041095042&partnerID=8YFLogxK
U2 - 10.1002/elsc.201700098
DO - 10.1002/elsc.201700098
M3 - Article
AN - SCOPUS:85041095042
VL - 18
SP - 263
EP - 268
JO - Engineering in life sciences
JF - Engineering in life sciences
SN - 1618-0240
IS - 4
ER -