Details
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Subtitle of host publication | uTAS 2007 |
| Editors | Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin |
| Pages | 808-810 |
| Number of pages | 3 |
| ISBN (electronic) | 9780979806407 |
| Publication status | Published - 2007 |
| Event | 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France Duration: 7 Oct 2007 → 11 Oct 2007 |
Abstract
This work reports on transport phenomena of proteins and cells observed in partitioning experiments with a novel setup for continuous-flow aqueous two-phase electrophoresis. After phase boundaries are formed in flow direction inside a microchannel, the system allows a continuous separation of proteins from cells. While proteins, here bovine serum albumin (BSA), overcome the phase boundary and, supported by an electric field, leave the phase they have been dissolved in almost completely, lymphoplastoid cells are retained, thus allowing a continuous separation of proteins from cells.
Keywords
- Cells, Electrophoresis, Proteins, Separation, Two-phase flow
ASJC Scopus subject areas
- Chemical Engineering(all)
- Chemical Engineering (miscellaneous)
- Chemical Engineering(all)
- Bioengineering
Cite this
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Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences: uTAS 2007. ed. / Jean-Louis Viovy; Patrick Tabeling; Stephanie Descroix; Laurent Malaquin. 2007. p. 808-810.
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Continuous separation of proteins and cells by two-phase electrophoresis in microchannels
AU - Münchow, G.
AU - Hardt, S.
AU - Kutter, J. P.
AU - Drese, K. S.
N1 - Publisher Copyright: © 2007 CBMS.
PY - 2007
Y1 - 2007
N2 - This work reports on transport phenomena of proteins and cells observed in partitioning experiments with a novel setup for continuous-flow aqueous two-phase electrophoresis. After phase boundaries are formed in flow direction inside a microchannel, the system allows a continuous separation of proteins from cells. While proteins, here bovine serum albumin (BSA), overcome the phase boundary and, supported by an electric field, leave the phase they have been dissolved in almost completely, lymphoplastoid cells are retained, thus allowing a continuous separation of proteins from cells.
AB - This work reports on transport phenomena of proteins and cells observed in partitioning experiments with a novel setup for continuous-flow aqueous two-phase electrophoresis. After phase boundaries are formed in flow direction inside a microchannel, the system allows a continuous separation of proteins from cells. While proteins, here bovine serum albumin (BSA), overcome the phase boundary and, supported by an electric field, leave the phase they have been dissolved in almost completely, lymphoplastoid cells are retained, thus allowing a continuous separation of proteins from cells.
KW - Cells
KW - Electrophoresis
KW - Proteins
KW - Separation
KW - Two-phase flow
UR - http://www.scopus.com/inward/record.url?scp=85088677962&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85088677962
SP - 808
EP - 810
BT - Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences
A2 - Viovy, Jean-Louis
A2 - Tabeling, Patrick
A2 - Descroix, Stephanie
A2 - Malaquin, Laurent
T2 - 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Y2 - 7 October 2007 through 11 October 2007
ER -