Details
| Original language | English |
|---|---|
| Pages (from-to) | 642-649 |
| Number of pages | 8 |
| Journal | International Journal of Artificial Organs |
| Volume | 47 |
| Issue number | 8 |
| Early online date | 21 Aug 2024 |
| Publication status | Published - Aug 2024 |
Abstract
Thick honeycomb-like electrospun scaffold with nanoparticles of hydroxyapatite (nHA) recently demonstrated its potential to promote proliferation and differentiation of a murine embryonic cell line (C3H10T1/2) to osteoblasts. In order to distinguish the respective effects of the structure and the composition on cell differentiation, beads-on-string fibers were used to manufacture thick honeycomb-like scaffolds without nHA. Mechanical and biological impacts of those beads-on string fibers were evaluated. Uniaxial tensile test showed that beads-on-string fibers decreased the Young Modulus and maximal stress but kept them appropriate for tissue engineering. C3H10T1/2 were seeded and cultured for 6 days on the scaffolds without any growth factors. Viability assays revealed the biocompatibility of the beads-on-string scaffolds, with adequate cells-materials interactions observed by confocal microscopy. Alkaline phosphatase staining was performed at day 6 in order to compare the early differentiation of cells to bone fate. The measure of stained area and intensity confirmed the beneficial effect of both honeycomb structure and nHA, independently. Finally, we showed that honeycomb-like electrospun scaffolds could be relevant candidates for promoting bone fate to cells in the absence of nHA. It offers an easier and faster manufacture process, in particular in bone-interface tissue engineering, permitting to avoid the dispersion of nHA and their interaction with the other cells.
Keywords
- additive manufacturing, beads-on-string fibers, bioengineering, biomaterials and nanotechnology, bone tissue, cell-biomaterial interactions, electrospinning, hydroxyapatite nanoparticles, micro-structured material, Tissue engineering and regenerative medicine
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Medicine (miscellaneous)
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
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In: International Journal of Artificial Organs, Vol. 47, No. 8, 08.2024, p. 642-649.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Comparison of hydroxyapatite and honeycomb micro-structure in bone tissue engineering using electrospun beads-on-string fibers
AU - Rivoallan, Nicolas
AU - Mueller, Marc
AU - Baudequin, Timothée
AU - Vigneron, Pascale
AU - Hébraud, Anne
AU - Jellali, Rachid
AU - Dermigny, Quentin
AU - Le Goff, Anne
AU - Schlatter, Guy
AU - Glasmacher, Birgit
AU - Legallais, Cécile
N1 - Publisher Copyright: © The Author(s) 2024.
PY - 2024/8
Y1 - 2024/8
N2 - Thick honeycomb-like electrospun scaffold with nanoparticles of hydroxyapatite (nHA) recently demonstrated its potential to promote proliferation and differentiation of a murine embryonic cell line (C3H10T1/2) to osteoblasts. In order to distinguish the respective effects of the structure and the composition on cell differentiation, beads-on-string fibers were used to manufacture thick honeycomb-like scaffolds without nHA. Mechanical and biological impacts of those beads-on string fibers were evaluated. Uniaxial tensile test showed that beads-on-string fibers decreased the Young Modulus and maximal stress but kept them appropriate for tissue engineering. C3H10T1/2 were seeded and cultured for 6 days on the scaffolds without any growth factors. Viability assays revealed the biocompatibility of the beads-on-string scaffolds, with adequate cells-materials interactions observed by confocal microscopy. Alkaline phosphatase staining was performed at day 6 in order to compare the early differentiation of cells to bone fate. The measure of stained area and intensity confirmed the beneficial effect of both honeycomb structure and nHA, independently. Finally, we showed that honeycomb-like electrospun scaffolds could be relevant candidates for promoting bone fate to cells in the absence of nHA. It offers an easier and faster manufacture process, in particular in bone-interface tissue engineering, permitting to avoid the dispersion of nHA and their interaction with the other cells.
AB - Thick honeycomb-like electrospun scaffold with nanoparticles of hydroxyapatite (nHA) recently demonstrated its potential to promote proliferation and differentiation of a murine embryonic cell line (C3H10T1/2) to osteoblasts. In order to distinguish the respective effects of the structure and the composition on cell differentiation, beads-on-string fibers were used to manufacture thick honeycomb-like scaffolds without nHA. Mechanical and biological impacts of those beads-on string fibers were evaluated. Uniaxial tensile test showed that beads-on-string fibers decreased the Young Modulus and maximal stress but kept them appropriate for tissue engineering. C3H10T1/2 were seeded and cultured for 6 days on the scaffolds without any growth factors. Viability assays revealed the biocompatibility of the beads-on-string scaffolds, with adequate cells-materials interactions observed by confocal microscopy. Alkaline phosphatase staining was performed at day 6 in order to compare the early differentiation of cells to bone fate. The measure of stained area and intensity confirmed the beneficial effect of both honeycomb structure and nHA, independently. Finally, we showed that honeycomb-like electrospun scaffolds could be relevant candidates for promoting bone fate to cells in the absence of nHA. It offers an easier and faster manufacture process, in particular in bone-interface tissue engineering, permitting to avoid the dispersion of nHA and their interaction with the other cells.
KW - additive manufacturing
KW - beads-on-string fibers
KW - bioengineering
KW - biomaterials and nanotechnology
KW - bone tissue
KW - cell-biomaterial interactions
KW - electrospinning
KW - hydroxyapatite nanoparticles
KW - micro-structured material
KW - Tissue engineering and regenerative medicine
UR - http://www.scopus.com/inward/record.url?scp=85201823130&partnerID=8YFLogxK
U2 - 10.48550/arXiv.2410.14226
DO - 10.48550/arXiv.2410.14226
M3 - Article
C2 - 39166430
AN - SCOPUS:85201823130
VL - 47
SP - 642
EP - 649
JO - International Journal of Artificial Organs
JF - International Journal of Artificial Organs
SN - 0391-3988
IS - 8
ER -