Details
| Original language | English |
|---|---|
| Article number | 58 |
| Journal | PLANTA |
| Volume | 257 |
| Issue number | 3 |
| Early online date | 16 Feb 2023 |
| Publication status | Published - Mar 2023 |
Abstract
Main conclusion: Nitrogen deficient and drought-tolerant or sensitive potatoes differ in proteomic responses under combined (NWD) and individual stresses. The sensitive genotype ‘Kiebitz’ exhibits a higher abundance of proteases under NWD. Abstract: Abiotic stresses such as N deficiency and drought affect the yield of Solanum tuberosum L. tremendously. Therefore, it is of importance to improve potato genotypes in terms of stress tolerance. In this study, we identified differentially abundant proteins (DAPs) in four starch potato genotypes under N deficiency (ND), drought stress (WD), or combined stress (NWD) in two rain-out shelter experiments. The gel-free LC–MS analysis generated a set of 1177 identified and quantified proteins. The incidence of common DAPs in tolerant and sensitive genotypes under NWD indicates general responses to this stress combination. Most of these proteins were part of the amino acid metabolism (13.9%). Three isoforms of S-adenosyl methionine synthase (SAMS) were found to be lower abundant in all genotypes. As SAMS were found upon application of single stresses as well, these proteins appear to be part of the general stress response in potato. Interestingly, the sensitive genotype ‘Kiebitz’ showed a higher abundance of three proteases (subtilase, carboxypeptidase, subtilase family protein) and a lower abundance of a protease inhibitor (stigma expressed protein) under NWD stress compared to control plants. The comparably tolerant genotype ‘Tomba’, however, displayed lower abundances of proteases. This indicates a better coping strategy for the tolerant genotype and a quicker reaction to WD when previously stressed with ND.
Keywords
- Abiotic stress, Combined stress, Label-free quantification, LC–MS, Protease, Solanum tuberosum, Stress response
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: PLANTA, Vol. 257, No. 3, 58, 03.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Combined nitrogen and drought stress leads to overlapping and unique proteomic responses in potato
AU - Wellpott, Katharina
AU - Jozefowicz, Anna M.
AU - Meise, Philipp
AU - Schum, Annegret
AU - Seddig, Sylvia
AU - Mock, Hans-Peter
AU - Winkelmann, Traud
AU - Bündig, Christin
N1 - Funding Information: This study was financed by the Federal Ministry of Food and Agriculture (BMEL) through the Agency of Renewable Resources (FNR) (FKZ: 22001917 and 22007018). The authors have no competing interests to declare that are relevant to the content of this article. The authors declare that no human and/or animal material, data, or cell lines were used in this study.
PY - 2023/3
Y1 - 2023/3
N2 - Main conclusion: Nitrogen deficient and drought-tolerant or sensitive potatoes differ in proteomic responses under combined (NWD) and individual stresses. The sensitive genotype ‘Kiebitz’ exhibits a higher abundance of proteases under NWD. Abstract: Abiotic stresses such as N deficiency and drought affect the yield of Solanum tuberosum L. tremendously. Therefore, it is of importance to improve potato genotypes in terms of stress tolerance. In this study, we identified differentially abundant proteins (DAPs) in four starch potato genotypes under N deficiency (ND), drought stress (WD), or combined stress (NWD) in two rain-out shelter experiments. The gel-free LC–MS analysis generated a set of 1177 identified and quantified proteins. The incidence of common DAPs in tolerant and sensitive genotypes under NWD indicates general responses to this stress combination. Most of these proteins were part of the amino acid metabolism (13.9%). Three isoforms of S-adenosyl methionine synthase (SAMS) were found to be lower abundant in all genotypes. As SAMS were found upon application of single stresses as well, these proteins appear to be part of the general stress response in potato. Interestingly, the sensitive genotype ‘Kiebitz’ showed a higher abundance of three proteases (subtilase, carboxypeptidase, subtilase family protein) and a lower abundance of a protease inhibitor (stigma expressed protein) under NWD stress compared to control plants. The comparably tolerant genotype ‘Tomba’, however, displayed lower abundances of proteases. This indicates a better coping strategy for the tolerant genotype and a quicker reaction to WD when previously stressed with ND.
AB - Main conclusion: Nitrogen deficient and drought-tolerant or sensitive potatoes differ in proteomic responses under combined (NWD) and individual stresses. The sensitive genotype ‘Kiebitz’ exhibits a higher abundance of proteases under NWD. Abstract: Abiotic stresses such as N deficiency and drought affect the yield of Solanum tuberosum L. tremendously. Therefore, it is of importance to improve potato genotypes in terms of stress tolerance. In this study, we identified differentially abundant proteins (DAPs) in four starch potato genotypes under N deficiency (ND), drought stress (WD), or combined stress (NWD) in two rain-out shelter experiments. The gel-free LC–MS analysis generated a set of 1177 identified and quantified proteins. The incidence of common DAPs in tolerant and sensitive genotypes under NWD indicates general responses to this stress combination. Most of these proteins were part of the amino acid metabolism (13.9%). Three isoforms of S-adenosyl methionine synthase (SAMS) were found to be lower abundant in all genotypes. As SAMS were found upon application of single stresses as well, these proteins appear to be part of the general stress response in potato. Interestingly, the sensitive genotype ‘Kiebitz’ showed a higher abundance of three proteases (subtilase, carboxypeptidase, subtilase family protein) and a lower abundance of a protease inhibitor (stigma expressed protein) under NWD stress compared to control plants. The comparably tolerant genotype ‘Tomba’, however, displayed lower abundances of proteases. This indicates a better coping strategy for the tolerant genotype and a quicker reaction to WD when previously stressed with ND.
KW - Abiotic stress
KW - Combined stress
KW - Label-free quantification
KW - LC–MS
KW - Protease
KW - Solanum tuberosum
KW - Stress response
UR - http://www.scopus.com/inward/record.url?scp=85148307445&partnerID=8YFLogxK
U2 - 10.1007/s00425-023-04085-4
DO - 10.1007/s00425-023-04085-4
M3 - Article
C2 - 36795167
AN - SCOPUS:85148307445
VL - 257
JO - PLANTA
JF - PLANTA
SN - 0032-0935
IS - 3
M1 - 58
ER -