Details
| Original language | English |
|---|---|
| Pages (from-to) | 334-338 |
| Number of pages | 5 |
| Journal | Journal of inorganic biochemistry |
| Volume | 153 |
| Publication status | Published - 3 Sept 2015 |
| Externally published | Yes |
Abstract
The influence of the surface charge and the state of agglomeration of ZnO nanoparticles on cellular uptake and viability are investigated. For this purpose, ZnO nanoparticles were synthesized by colloidal routes and their physicochemical properties were investigated in detail. Three different surface modifications were investigated, involving coatings with the amphiphilic polymer poly(isobutylene-alt-maleic anhydride)-graft-dodecyl, mercaptoundecanoic acid, and l-arginine, which provide the nanoparticles with either a negative or a positive zeta-potential. The hydrodynamic diameters and zeta-potentials of all three nanoparticle species were investigated at different pH values and NaCl concentrations by means of dynamic light scattering and laser Doppler anemometry, respectively. The three differently modified ZnO nanoparticle species of similar sizes were also investigated in respect to their cellular uptake by 3T3 fibroblasts and HeLa cells, and their effect on cell viability.
Keywords
- Cytotoxicity, Inorganic nanoparticles, Organic surface coating
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Inorganic Chemistry
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In: Journal of inorganic biochemistry, Vol. 153, 03.09.2015, p. 334-338.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Charge and agglomeration dependent in vitro uptake and cytotoxicity of zinc oxide nanoparticles
AU - Abdelmonem, Abuelmagd M.
AU - Pelaz, Beatriz
AU - Kantner, Karsten
AU - Bigall, Nadja C.
AU - Del Pino, Pablo
AU - Parak, Wolfgang J.
N1 - Funding information: This project was supported by the German Research Foundation ( DFG GRK 1782 to WJP and NCB). BP acknowledges the financial support from the Alexander von Humboldt Foundation . NB acknowledges the NanoMatFutur grant number 03X5525 .
PY - 2015/9/3
Y1 - 2015/9/3
N2 - The influence of the surface charge and the state of agglomeration of ZnO nanoparticles on cellular uptake and viability are investigated. For this purpose, ZnO nanoparticles were synthesized by colloidal routes and their physicochemical properties were investigated in detail. Three different surface modifications were investigated, involving coatings with the amphiphilic polymer poly(isobutylene-alt-maleic anhydride)-graft-dodecyl, mercaptoundecanoic acid, and l-arginine, which provide the nanoparticles with either a negative or a positive zeta-potential. The hydrodynamic diameters and zeta-potentials of all three nanoparticle species were investigated at different pH values and NaCl concentrations by means of dynamic light scattering and laser Doppler anemometry, respectively. The three differently modified ZnO nanoparticle species of similar sizes were also investigated in respect to their cellular uptake by 3T3 fibroblasts and HeLa cells, and their effect on cell viability.
AB - The influence of the surface charge and the state of agglomeration of ZnO nanoparticles on cellular uptake and viability are investigated. For this purpose, ZnO nanoparticles were synthesized by colloidal routes and their physicochemical properties were investigated in detail. Three different surface modifications were investigated, involving coatings with the amphiphilic polymer poly(isobutylene-alt-maleic anhydride)-graft-dodecyl, mercaptoundecanoic acid, and l-arginine, which provide the nanoparticles with either a negative or a positive zeta-potential. The hydrodynamic diameters and zeta-potentials of all three nanoparticle species were investigated at different pH values and NaCl concentrations by means of dynamic light scattering and laser Doppler anemometry, respectively. The three differently modified ZnO nanoparticle species of similar sizes were also investigated in respect to their cellular uptake by 3T3 fibroblasts and HeLa cells, and their effect on cell viability.
KW - Cytotoxicity
KW - Inorganic nanoparticles
KW - Organic surface coating
UR - http://www.scopus.com/inward/record.url?scp=84951567744&partnerID=8YFLogxK
U2 - 10.1016/j.jinorgbio.2015.08.029
DO - 10.1016/j.jinorgbio.2015.08.029
M3 - Article
C2 - 26387023
AN - SCOPUS:84951567744
VL - 153
SP - 334
EP - 338
JO - Journal of inorganic biochemistry
JF - Journal of inorganic biochemistry
SN - 0162-0134
ER -