Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Jörn Schaeske
  • Elena Fadeeva
  • Sabrina Schlie-Wolter
  • Andrea Deiwick
  • Boris N. Chichkov
  • Alexandra Ingendoh-Tsakmakidis
  • Meike Stiesch
  • Andreas Winkel

Research Organisations

External Research Organisations

  • Hannover Medical School (MHH)
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Details

Original languageEnglish
Article number8442
Pages (from-to)1-19
Number of pages19
JournalInternational Journal of Molecular Sciences
Volume21
Issue number22
Publication statusPublished - 10 Nov 2020

Abstract

Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.

Keywords

    Biomaterials, Cell exclusion assay, Cell proliferation, Cell spreading, Cytocompatibility, Focal adhesion, In vitro screening, Primary vs. immortalized cell lines, Spike structures

ASJC Scopus subject areas

Cite this

Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces. / Schaeske, Jörn; Fadeeva, Elena; Schlie-Wolter, Sabrina et al.
In: International Journal of Molecular Sciences, Vol. 21, No. 22, 8442, 10.11.2020, p. 1-19.

Research output: Contribution to journalArticleResearchpeer review

Schaeske, J, Fadeeva, E, Schlie-Wolter, S, Deiwick, A, Chichkov, BN, Ingendoh-Tsakmakidis, A, Stiesch, M & Winkel, A 2020, 'Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces', International Journal of Molecular Sciences, vol. 21, no. 22, 8442, pp. 1-19. https://doi.org/10.3390/ijms21228442
Schaeske, J., Fadeeva, E., Schlie-Wolter, S., Deiwick, A., Chichkov, B. N., Ingendoh-Tsakmakidis, A., Stiesch, M., & Winkel, A. (2020). Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces. International Journal of Molecular Sciences, 21(22), 1-19. Article 8442. https://doi.org/10.3390/ijms21228442
Schaeske J, Fadeeva E, Schlie-Wolter S, Deiwick A, Chichkov BN, Ingendoh-Tsakmakidis A et al. Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces. International Journal of Molecular Sciences. 2020 Nov 10;21(22):1-19. 8442. doi: 10.3390/ijms21228442
Schaeske, Jörn ; Fadeeva, Elena ; Schlie-Wolter, Sabrina et al. / Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces. In: International Journal of Molecular Sciences. 2020 ; Vol. 21, No. 22. pp. 1-19.
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abstract = "Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.",
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AU - Schaeske, Jörn

AU - Fadeeva, Elena

AU - Schlie-Wolter, Sabrina

AU - Deiwick, Andrea

AU - Chichkov, Boris N.

AU - Ingendoh-Tsakmakidis, Alexandra

AU - Stiesch, Meike

AU - Winkel, Andreas

N1 - Funding Information: Acknowledgments: We would like to thank the research association BIOFABRICATION for NIFE, sponsored by the government of Lower Saxony, and the Volkswagenstiftung, for funding this project. We would also like to thank the Research Workshop of the Medical School Hannover for the construction of the frames for the optimized migration assay and Annike Brune for the technical illustration regarding the migration assay in the material and methods section. Funding Information: Niedersächsisches Ministerium für Wissenschaft und Kultur together with Volkswagen Foundation: WVZN2860.

PY - 2020/11/10

Y1 - 2020/11/10

N2 - Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.

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KW - Spike structures

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