Blue-native PAGE in plants: A tool in analysis of protein-protein interactions

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  • University of Western Australia
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Original languageEnglish
Article number11
JournalPLANT METHODS
Volume1
Issue number11
Publication statusPublished - 16 Nov 2005

Abstract

Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.

Keywords

    2D-PAGE, Coomassie, Gel-based Proteomics, hydrophobic proteins, protein complexes, solubilization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics
  • Agricultural and Biological Sciences(all)
  • Plant Science

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Blue-native PAGE in plants: A tool in analysis of protein-protein interactions. / Eubel, Holger; Braun, Hans Peter; Millar, A. Harvey.
In: PLANT METHODS, Vol. 1, No. 11, 11, 16.11.2005.

Research output: Contribution to journalReview articleResearchpeer review

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