Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure

Research output: Contribution to journalShort/Brief/Rapid CommunicationResearchpeer review

Authors

  • Zeinab Fneish
  • Jennifer Becker
  • Felix Mulenge
  • Firas Fneish
  • Bibiana Costa
  • Claudia Traidl-Hoffmann
  • Stefanie Gilles
  • Ulrich Kalinke

External Research Organisations

  • TWINCORE Zentrum für Experimentelle und Klinische Infektionsforschung GmbH
  • University of Augsburg
  • Helmholtz Zentrum München - German Research Center for Environmental Health
  • Christine-Kühne Center for Allergy Research and Education (CK-Care)
  • Hannover Medical School (MHH)
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Details

Original languageEnglish
Article number148649
Number of pages7
JournalGENE
Volume927
Early online date7 Jun 2024
Publication statusE-pub ahead of print - 7 Jun 2024

Abstract

During the birch pollen season an enhanced incidence of virus infections is noticed, raising the question whether pollen can affect anti-viral responses independent of allergic reactions. We previously showed that birch pollen-treatment of monocyte-derived dendritic cells (moDC) enhances human cytomegalovirus (HCMV) infection. Here we addressed how in moDC the relatively weak pollen response can affect the comparably strong response to HCMV. To this end, moDC were stimulated with aqueous birch pollen extract (APE), HCMV, and APE with HCMV, and transcriptomic signatures were determined after 6 and 24 h of incubation. Infection was monitored upon exposure of moDC to GFP expressing HCMV by flow cytometric analysis of GFP expressing cells. Principle component analysis of RNA sequencing data revealed close clustering of mock and APE treated moDC, whereas HCMV as well as APE with HCMV treated moDC clustered separately after 6 and 24 h of incubation, respectively. Communally induced genes were detected in APE, HCMV and APE with HCMV treated moDC. In APE with HCMV treated moDC, the comparably weak APE induced signatures were maintained after HCMV exposure. In particular, NF-κB/RELA and PI3K/AKT/MAPK signaling were altered upon APE with HCMV exposure. Earlier, we discovered that NF-κB inhibition alleviated APE induced enhancement of HCMV infection. Here we additionally found that impairment of PI3K signaling reduced HCMV infection in HCMV and APE with HCMV treated moDC. APE treated moDC that were exposed to HCMV show a unique host gene signature, which to a large extent is regulated by NF-κB activation and PI3K/AKT/MAPK signaling.

Keywords

    Antiviral immunity, Aqueous birch pollen extract, Monocyte-derived dendritic cells, PI3K signaling, Pro-inflammatory cytokines, RNA-sequencing, Viral infection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics

Cite this

Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure. / Fneish, Zeinab; Becker, Jennifer; Mulenge, Felix et al.
In: GENE, Vol. 927, 148649, 15.11.2024.

Research output: Contribution to journalShort/Brief/Rapid CommunicationResearchpeer review

Fneish, Z, Becker, J, Mulenge, F, Fneish, F, Costa, B, Traidl-Hoffmann, C, Gilles, S & Kalinke, U 2024, 'Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure', GENE, vol. 927, 148649. https://doi.org/10.1016/j.gene.2024.148649
Fneish, Z., Becker, J., Mulenge, F., Fneish, F., Costa, B., Traidl-Hoffmann, C., Gilles, S., & Kalinke, U. (2024). Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure. GENE, 927, Article 148649. Advance online publication. https://doi.org/10.1016/j.gene.2024.148649
Fneish Z, Becker J, Mulenge F, Fneish F, Costa B, Traidl-Hoffmann C et al. Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure. GENE. 2024 Nov 15;927:148649. Epub 2024 Jun 7. doi: 10.1016/j.gene.2024.148649
Fneish, Zeinab ; Becker, Jennifer ; Mulenge, Felix et al. / Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure. In: GENE. 2024 ; Vol. 927.
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title = "Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure",
abstract = "During the birch pollen season an enhanced incidence of virus infections is noticed, raising the question whether pollen can affect anti-viral responses independent of allergic reactions. We previously showed that birch pollen-treatment of monocyte-derived dendritic cells (moDC) enhances human cytomegalovirus (HCMV) infection. Here we addressed how in moDC the relatively weak pollen response can affect the comparably strong response to HCMV. To this end, moDC were stimulated with aqueous birch pollen extract (APE), HCMV, and APE with HCMV, and transcriptomic signatures were determined after 6 and 24 h of incubation. Infection was monitored upon exposure of moDC to GFP expressing HCMV by flow cytometric analysis of GFP expressing cells. Principle component analysis of RNA sequencing data revealed close clustering of mock and APE treated moDC, whereas HCMV as well as APE with HCMV treated moDC clustered separately after 6 and 24 h of incubation, respectively. Communally induced genes were detected in APE, HCMV and APE with HCMV treated moDC. In APE with HCMV treated moDC, the comparably weak APE induced signatures were maintained after HCMV exposure. In particular, NF-κB/RELA and PI3K/AKT/MAPK signaling were altered upon APE with HCMV exposure. Earlier, we discovered that NF-κB inhibition alleviated APE induced enhancement of HCMV infection. Here we additionally found that impairment of PI3K signaling reduced HCMV infection in HCMV and APE with HCMV treated moDC. APE treated moDC that were exposed to HCMV show a unique host gene signature, which to a large extent is regulated by NF-κB activation and PI3K/AKT/MAPK signaling.",
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TY - JOUR

T1 - Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure

AU - Fneish, Zeinab

AU - Becker, Jennifer

AU - Mulenge, Felix

AU - Fneish, Firas

AU - Costa, Bibiana

AU - Traidl-Hoffmann, Claudia

AU - Gilles, Stefanie

AU - Kalinke, Ulrich

N1 - Publisher Copyright: © 2024 The Author(s)

PY - 2024/6/7

Y1 - 2024/6/7

N2 - During the birch pollen season an enhanced incidence of virus infections is noticed, raising the question whether pollen can affect anti-viral responses independent of allergic reactions. We previously showed that birch pollen-treatment of monocyte-derived dendritic cells (moDC) enhances human cytomegalovirus (HCMV) infection. Here we addressed how in moDC the relatively weak pollen response can affect the comparably strong response to HCMV. To this end, moDC were stimulated with aqueous birch pollen extract (APE), HCMV, and APE with HCMV, and transcriptomic signatures were determined after 6 and 24 h of incubation. Infection was monitored upon exposure of moDC to GFP expressing HCMV by flow cytometric analysis of GFP expressing cells. Principle component analysis of RNA sequencing data revealed close clustering of mock and APE treated moDC, whereas HCMV as well as APE with HCMV treated moDC clustered separately after 6 and 24 h of incubation, respectively. Communally induced genes were detected in APE, HCMV and APE with HCMV treated moDC. In APE with HCMV treated moDC, the comparably weak APE induced signatures were maintained after HCMV exposure. In particular, NF-κB/RELA and PI3K/AKT/MAPK signaling were altered upon APE with HCMV exposure. Earlier, we discovered that NF-κB inhibition alleviated APE induced enhancement of HCMV infection. Here we additionally found that impairment of PI3K signaling reduced HCMV infection in HCMV and APE with HCMV treated moDC. APE treated moDC that were exposed to HCMV show a unique host gene signature, which to a large extent is regulated by NF-κB activation and PI3K/AKT/MAPK signaling.

AB - During the birch pollen season an enhanced incidence of virus infections is noticed, raising the question whether pollen can affect anti-viral responses independent of allergic reactions. We previously showed that birch pollen-treatment of monocyte-derived dendritic cells (moDC) enhances human cytomegalovirus (HCMV) infection. Here we addressed how in moDC the relatively weak pollen response can affect the comparably strong response to HCMV. To this end, moDC were stimulated with aqueous birch pollen extract (APE), HCMV, and APE with HCMV, and transcriptomic signatures were determined after 6 and 24 h of incubation. Infection was monitored upon exposure of moDC to GFP expressing HCMV by flow cytometric analysis of GFP expressing cells. Principle component analysis of RNA sequencing data revealed close clustering of mock and APE treated moDC, whereas HCMV as well as APE with HCMV treated moDC clustered separately after 6 and 24 h of incubation, respectively. Communally induced genes were detected in APE, HCMV and APE with HCMV treated moDC. In APE with HCMV treated moDC, the comparably weak APE induced signatures were maintained after HCMV exposure. In particular, NF-κB/RELA and PI3K/AKT/MAPK signaling were altered upon APE with HCMV exposure. Earlier, we discovered that NF-κB inhibition alleviated APE induced enhancement of HCMV infection. Here we additionally found that impairment of PI3K signaling reduced HCMV infection in HCMV and APE with HCMV treated moDC. APE treated moDC that were exposed to HCMV show a unique host gene signature, which to a large extent is regulated by NF-κB activation and PI3K/AKT/MAPK signaling.

KW - Antiviral immunity

KW - Aqueous birch pollen extract

KW - Monocyte-derived dendritic cells

KW - PI3K signaling

KW - Pro-inflammatory cytokines

KW - RNA-sequencing

KW - Viral infection

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U2 - 10.1016/j.gene.2024.148649

DO - 10.1016/j.gene.2024.148649

M3 - Short/Brief/Rapid Communication

C2 - 38852697

AN - SCOPUS:85195678326

VL - 927

JO - GENE

JF - GENE

SN - 0378-1119

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