Details
| Original language | English |
|---|---|
| Pages (from-to) | 201-210 |
| Number of pages | 10 |
| Journal | Analytica chimica acta |
| Volume | 238 |
| Issue number | C |
| Publication status | Published - 1990 |
Abstract
The development of enzyme-modified bio-field-effect transistors (BioFETs) for the determination of glucose, urea, penicillin G, penicillin V and cephalosporin C is reported. BioFETs are produced by covering the pH-sensitive gate areas of ion-selective field-effect transistors with enzyme membranes. The characteristics of the resulting BioFETs and the influence of several parameters, e.g., pH and buffer capacity, are described. The measuring range covers 1-2 orders of magnitude of substrate concentration, and the BioFETs are applicable for 3-12 weeks, depending on the enzyme. They show a short response time and are well suited for detection in flow systems. The frequency of determination with BioFETs in flow systems is high (15-20 measurements per hour). The application of a BioFET in on-line bioprocess control is described. A glucose oxidase FET monitors the glucose concentration during cultivation of Escherichia coli. The results correspond well with off-line liquid chromatographic determinations.
Keywords
- Biosensors, Cephalosporin C, Field-effect transistors, Glucose, Penicillin, Process analysis, Urea
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Environmental Science(all)
- Environmental Chemistry
- Chemistry(all)
- Spectroscopy
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In: Analytica chimica acta, Vol. 238, No. C, 1990, p. 201-210.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Bio-field-effect transistors as detectors in flow-injection analysis
AU - Brand, U.
AU - Reinhardt, B.
AU - Rüther, F.
AU - Scheper, T.
AU - Schügerl, K.
N1 - Funding information: The authors thank Dr. R. Howind of Eppen-dorf, Hamburg, for his cooperation concerning the FIA system. The financial support of the Bundesministerium fur Forschung und Technolo-gie of the Federal Republic of Germany is gratefully acknowledged.
PY - 1990
Y1 - 1990
N2 - The development of enzyme-modified bio-field-effect transistors (BioFETs) for the determination of glucose, urea, penicillin G, penicillin V and cephalosporin C is reported. BioFETs are produced by covering the pH-sensitive gate areas of ion-selective field-effect transistors with enzyme membranes. The characteristics of the resulting BioFETs and the influence of several parameters, e.g., pH and buffer capacity, are described. The measuring range covers 1-2 orders of magnitude of substrate concentration, and the BioFETs are applicable for 3-12 weeks, depending on the enzyme. They show a short response time and are well suited for detection in flow systems. The frequency of determination with BioFETs in flow systems is high (15-20 measurements per hour). The application of a BioFET in on-line bioprocess control is described. A glucose oxidase FET monitors the glucose concentration during cultivation of Escherichia coli. The results correspond well with off-line liquid chromatographic determinations.
AB - The development of enzyme-modified bio-field-effect transistors (BioFETs) for the determination of glucose, urea, penicillin G, penicillin V and cephalosporin C is reported. BioFETs are produced by covering the pH-sensitive gate areas of ion-selective field-effect transistors with enzyme membranes. The characteristics of the resulting BioFETs and the influence of several parameters, e.g., pH and buffer capacity, are described. The measuring range covers 1-2 orders of magnitude of substrate concentration, and the BioFETs are applicable for 3-12 weeks, depending on the enzyme. They show a short response time and are well suited for detection in flow systems. The frequency of determination with BioFETs in flow systems is high (15-20 measurements per hour). The application of a BioFET in on-line bioprocess control is described. A glucose oxidase FET monitors the glucose concentration during cultivation of Escherichia coli. The results correspond well with off-line liquid chromatographic determinations.
KW - Biosensors
KW - Cephalosporin C
KW - Field-effect transistors
KW - Glucose
KW - Penicillin
KW - Process analysis
KW - Urea
UR - http://www.scopus.com/inward/record.url?scp=0025086654&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(00)80538-3
DO - 10.1016/S0003-2670(00)80538-3
M3 - Article
AN - SCOPUS:0025086654
VL - 238
SP - 201
EP - 210
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - C
ER -