Details
Original language | English |
---|---|
Article number | 167458 |
Journal | Science of the Total Environment |
Issue number | 905 |
Early online date | 28 Sept 2023 |
Publication status | Published - 20 Dec 2023 |
Abstract
Biofilms form on any available surface and, depending on the characteristics of the material and the environmental conditions, biodegradation can take place. We compared the bacterial composition of polyhydroxybutyrate (PHB)-related biofilm communities from marine ex-situ and in-situ tests to assess the differences in diversity and abundance between these two biofilms. This comparison will help to better assess the transferability of tank tests to real-life scenarios. The in-situ tests were set up in the Mediterranean Sea on the Island of Elba, Italy where PHB-tensile bars were lodged in the sediments. This created a water-exposed aerobic and mud-planted anaerobic scenario. The ex-situ tests were modeled after in-situ tests and performed in temperature-controlled tanks. The PHB-related biofilms were harvested after 240 days of exposure along with planktonic bacteria, and particle- and sediment-related biofilm. The bacterial composition was elucidated using 16S rDNA sequencing. Biofilms harvested from the in-situ test were more diverse, less even, and contained more rare species compared to biofilms from the ex-situ test. The PHB-related biofilm was characterized by a higher abundance of the bacterial order Desulfobacterales. The composition of PHB-related biofilm varied significantly between the two tests and between aerobic and anaerobic conditions. The composition of PHB-related biofilm was significantly different from planktonic bacteria, particle, and sediment-related biofilm, showing the influence of PHB on the biofilm composition. Thus, the ex-situ tank test for PHB degradation cannot, in terms of bacterial composition, simulate the in-situ conditions to their full extent.
Keywords
- Bacterial diversity, Biofilm, Ex-situ test, In-situ test, Marine conditions, Polyhydroxybutyrate
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Engineering
- Environmental Science(all)
- Environmental Chemistry
- Environmental Science(all)
- Waste Management and Disposal
- Environmental Science(all)
- Pollution
Sustainable Development Goals
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In: Science of the Total Environment, No. 905, 167458, 20.12.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Bacterial diversity of biofilms on polyhydroxybutyrate exposed to marine conditions
T2 - Ex-situ vs. in-situ tests
AU - Bhalerao, Aniruddha
AU - Dueker, Urda
AU - Weber, Miriam
AU - Eich, Andreas
AU - Lott, Christian
AU - Endres, Hans Josef
AU - Nogueira, Regina
N1 - Funding Information: This research was funded by the Federal Ministry of Food and Agriculture , Germany (BMEL) via the Agency for Renewable Resources (FNR) (project numbers: 22025118 and 22028618 ). Mr. Bhalerao was supported by the Research Grants - Doctoral Programmes in Germany, 2019/2020 from the German Academic Exchange Service (DAAD, funding ID: 57440921 ). Funding Information: The computational part of this study was supported by the cluster system at the Leibniz University Hannover , Germany. We would like to thank the support team for their prompt and productive input.
PY - 2023/12/20
Y1 - 2023/12/20
N2 - Biofilms form on any available surface and, depending on the characteristics of the material and the environmental conditions, biodegradation can take place. We compared the bacterial composition of polyhydroxybutyrate (PHB)-related biofilm communities from marine ex-situ and in-situ tests to assess the differences in diversity and abundance between these two biofilms. This comparison will help to better assess the transferability of tank tests to real-life scenarios. The in-situ tests were set up in the Mediterranean Sea on the Island of Elba, Italy where PHB-tensile bars were lodged in the sediments. This created a water-exposed aerobic and mud-planted anaerobic scenario. The ex-situ tests were modeled after in-situ tests and performed in temperature-controlled tanks. The PHB-related biofilms were harvested after 240 days of exposure along with planktonic bacteria, and particle- and sediment-related biofilm. The bacterial composition was elucidated using 16S rDNA sequencing. Biofilms harvested from the in-situ test were more diverse, less even, and contained more rare species compared to biofilms from the ex-situ test. The PHB-related biofilm was characterized by a higher abundance of the bacterial order Desulfobacterales. The composition of PHB-related biofilm varied significantly between the two tests and between aerobic and anaerobic conditions. The composition of PHB-related biofilm was significantly different from planktonic bacteria, particle, and sediment-related biofilm, showing the influence of PHB on the biofilm composition. Thus, the ex-situ tank test for PHB degradation cannot, in terms of bacterial composition, simulate the in-situ conditions to their full extent.
AB - Biofilms form on any available surface and, depending on the characteristics of the material and the environmental conditions, biodegradation can take place. We compared the bacterial composition of polyhydroxybutyrate (PHB)-related biofilm communities from marine ex-situ and in-situ tests to assess the differences in diversity and abundance between these two biofilms. This comparison will help to better assess the transferability of tank tests to real-life scenarios. The in-situ tests were set up in the Mediterranean Sea on the Island of Elba, Italy where PHB-tensile bars were lodged in the sediments. This created a water-exposed aerobic and mud-planted anaerobic scenario. The ex-situ tests were modeled after in-situ tests and performed in temperature-controlled tanks. The PHB-related biofilms were harvested after 240 days of exposure along with planktonic bacteria, and particle- and sediment-related biofilm. The bacterial composition was elucidated using 16S rDNA sequencing. Biofilms harvested from the in-situ test were more diverse, less even, and contained more rare species compared to biofilms from the ex-situ test. The PHB-related biofilm was characterized by a higher abundance of the bacterial order Desulfobacterales. The composition of PHB-related biofilm varied significantly between the two tests and between aerobic and anaerobic conditions. The composition of PHB-related biofilm was significantly different from planktonic bacteria, particle, and sediment-related biofilm, showing the influence of PHB on the biofilm composition. Thus, the ex-situ tank test for PHB degradation cannot, in terms of bacterial composition, simulate the in-situ conditions to their full extent.
KW - Bacterial diversity
KW - Biofilm
KW - Ex-situ test
KW - In-situ test
KW - Marine conditions
KW - Polyhydroxybutyrate
UR - http://www.scopus.com/inward/record.url?scp=85173557616&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2023.167458
DO - 10.1016/j.scitotenv.2023.167458
M3 - Article
C2 - 37777124
AN - SCOPUS:85173557616
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
IS - 905
M1 - 167458
ER -