Details
Original language | English |
---|---|
Pages (from-to) | 249-254 |
Number of pages | 6 |
Journal | IFAC Proceedings Volumes (IFAC-PapersOnline) |
Volume | 40 |
Issue number | 4 |
Publication status | Published - 2007 |
Event | 10th IFAC Symposium on Computer Applications in Biotechnology, 2007 - Cancun, Mexico Duration: 4 Jun 2007 → 6 Jun 2007 |
Abstract
In-situ-microscopy is an efficient method to measure cell concentration during cultivations. Furthermore the size distribution of the cells as well as their morphology can be determined on-line. To operate the microscope and acquire sequences of cell images a software system has been developed. This software also allows the on-line analysis of the image data to determine the cell concentration. The software, the image analysis algorithm as well as the analysis results of a yeast cell cultivation are presented. It is demonstrated, that the automated determination of the cell numbers agree very well with its manual count.
Keywords
- Cell concentration, Image analysis, In-situ microscopy, Yeast cell cultivation
ASJC Scopus subject areas
- Engineering(all)
- Control and Systems Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: IFAC Proceedings Volumes (IFAC-PapersOnline), Vol. 40, No. 4, 2007, p. 249-254.
Research output: Contribution to journal › Conference article › Research › peer review
}
TY - JOUR
T1 - Application of in-situ-microscopy and digital image processing in yeast cultivations
AU - Lindner, P.
AU - Krabichler, C.
AU - Rudolph, G.
AU - Scheper, T.
AU - Hitzmann, B.
PY - 2007
Y1 - 2007
N2 - In-situ-microscopy is an efficient method to measure cell concentration during cultivations. Furthermore the size distribution of the cells as well as their morphology can be determined on-line. To operate the microscope and acquire sequences of cell images a software system has been developed. This software also allows the on-line analysis of the image data to determine the cell concentration. The software, the image analysis algorithm as well as the analysis results of a yeast cell cultivation are presented. It is demonstrated, that the automated determination of the cell numbers agree very well with its manual count.
AB - In-situ-microscopy is an efficient method to measure cell concentration during cultivations. Furthermore the size distribution of the cells as well as their morphology can be determined on-line. To operate the microscope and acquire sequences of cell images a software system has been developed. This software also allows the on-line analysis of the image data to determine the cell concentration. The software, the image analysis algorithm as well as the analysis results of a yeast cell cultivation are presented. It is demonstrated, that the automated determination of the cell numbers agree very well with its manual count.
KW - Cell concentration
KW - Image analysis
KW - In-situ microscopy
KW - Yeast cell cultivation
UR - http://www.scopus.com/inward/record.url?scp=84944319558&partnerID=8YFLogxK
U2 - 10.3182/20070604-3-mx-2914.00043
DO - 10.3182/20070604-3-mx-2914.00043
M3 - Conference article
AN - SCOPUS:84944319558
VL - 40
SP - 249
EP - 254
JO - IFAC Proceedings Volumes (IFAC-PapersOnline)
JF - IFAC Proceedings Volumes (IFAC-PapersOnline)
SN - 1474-6670
IS - 4
T2 - 10th IFAC Symposium on Computer Applications in Biotechnology, 2007
Y2 - 4 June 2007 through 6 June 2007
ER -