Details
| Original language | English |
|---|---|
| Pages (from-to) | 25-31 |
| Number of pages | 7 |
| Journal | Analytica chimica acta |
| Volume | 300 |
| Issue number | 1-3 |
| Publication status | Published - 20 Jan 1995 |
| Externally published | Yes |
Abstract
Glucose, maltose, sucrose, lactose, ethanol and urea concentrations were monitored simultaneously during the cultivation of Escherichia coli and Saccharomyces cerevisiae by means of enzyme field effect transistors (EnFETs) applying glucose dehydrogenase (GDH), maltase (MAL)/GDH, invertase (INV)/GDH, β-galactosidase (β-GAL)/galactosedehydrogenase (GALDH), alcoholdehydrogenase (ADH)/aldehydedehydrogenase (ALDH), and urease. These enzymes were (co)immobilized on the pH sensitive gates of an eight-FET array. The FET array was integrated in a commercial FIA system.
Keywords
- Alcohol dehydrogenase, Aldehyde dehydrogenase, Field effect transistor (FET), Flow injection, Galactose dehydrogenase, Glucose dehydrogenase, Invertase, Maltase, Sensors, Urease, β-Galactosidase
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Environmental Science(all)
- Environmental Chemistry
- Chemistry(all)
- Spectroscopy
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In: Analytica chimica acta, Vol. 300, No. 1-3, 20.01.1995, p. 25-31.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Application of enzyme-field effect transistor sensor arrays as detectors in a flow-injection analysis system for simultaneous monitoring of medium components. Part II. Monitoring of cultivation processes
AU - Kullick, T.
AU - Bock, U.
AU - Schubert, J.
AU - Scheper, T.
AU - Schügerl, K.
PY - 1995/1/20
Y1 - 1995/1/20
N2 - Glucose, maltose, sucrose, lactose, ethanol and urea concentrations were monitored simultaneously during the cultivation of Escherichia coli and Saccharomyces cerevisiae by means of enzyme field effect transistors (EnFETs) applying glucose dehydrogenase (GDH), maltase (MAL)/GDH, invertase (INV)/GDH, β-galactosidase (β-GAL)/galactosedehydrogenase (GALDH), alcoholdehydrogenase (ADH)/aldehydedehydrogenase (ALDH), and urease. These enzymes were (co)immobilized on the pH sensitive gates of an eight-FET array. The FET array was integrated in a commercial FIA system.
AB - Glucose, maltose, sucrose, lactose, ethanol and urea concentrations were monitored simultaneously during the cultivation of Escherichia coli and Saccharomyces cerevisiae by means of enzyme field effect transistors (EnFETs) applying glucose dehydrogenase (GDH), maltase (MAL)/GDH, invertase (INV)/GDH, β-galactosidase (β-GAL)/galactosedehydrogenase (GALDH), alcoholdehydrogenase (ADH)/aldehydedehydrogenase (ALDH), and urease. These enzymes were (co)immobilized on the pH sensitive gates of an eight-FET array. The FET array was integrated in a commercial FIA system.
KW - Alcohol dehydrogenase
KW - Aldehyde dehydrogenase
KW - Field effect transistor (FET)
KW - Flow injection
KW - Galactose dehydrogenase
KW - Glucose dehydrogenase
KW - Invertase
KW - Maltase
KW - Sensors
KW - Urease
KW - β-Galactosidase
UR - http://www.scopus.com/inward/record.url?scp=0028868274&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(94)00376-W
DO - 10.1016/0003-2670(94)00376-W
M3 - Article
AN - SCOPUS:0028868274
VL - 300
SP - 25
EP - 31
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - 1-3
ER -