Antifungal activity of compounds isolated from Aspergillus niger and their molecular docking studies with tomatinase

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Zulqarnain
  • Zafar Iqbal
  • Russell Cox
  • Jawad Anwar
  • Nasir Ahmad
  • Khalid Khan
  • Mudassar Iqbal
  • Nazish Manzoor
  • Saeed Ullah Khattak

Research Organisations

External Research Organisations

  • The University of Agriculture, Peshawar
  • Islamia College University
  • University of Peshawar
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Details

Original languageEnglish
Pages (from-to)2642-2646
Number of pages5
JournalNatural product research
Volume34
Issue number18
Early online date24 Dec 2018
Publication statusPublished - 16 Sept 2020

Abstract

Using a dual culture antagonism assay, Aspergillus niger exhibited 51.5 ± 1.1% growth inhibition against Fusarium oxysporum f.sp. lycopersici, the wilt pathogen of tomato. For enhanced production of antifungal metabolites, nutrient optimization was performed and in vitro well-diffusion antifungal assays demonstrated that crude extract obtained from GPYB culture showed a maximum zone of inhibition (8.8 ± 0.4 mm) against the wilt pathogen, which is corroborated by the comparative LCMS profiles of the extracts from all three media i.e. GPYB, YEB and PDB. Two known compounds, Asperazine (m/z 665 [M + H]+) and Nigerone (m/z 571 [M + H]+), were isolated from A. niger and their antifungal activity is reported here for the first time. In MIC experiments, Asperazine and Nigerone inhibited the pathogen at 60 and 80 µg·mL−1 respectively. Molecular docking studies of Nigerone and Asperazine with F. oxysporum tomatinase showed five and six binding interactions respectively.

Keywords

    antifungal docking, Dual culture assay, fungicides, growth inhibition, LCMS

ASJC Scopus subject areas

Cite this

Antifungal activity of compounds isolated from Aspergillus niger and their molecular docking studies with tomatinase. / Zulqarnain; Iqbal, Zafar; Cox, Russell et al.
In: Natural product research, Vol. 34, No. 18, 16.09.2020, p. 2642-2646.

Research output: Contribution to journalArticleResearchpeer review

Zulqarnain, Iqbal, Z, Cox, R, Anwar, J, Ahmad, N, Khan, K, Iqbal, M, Manzoor, N & Khattak, SU 2020, 'Antifungal activity of compounds isolated from Aspergillus niger and their molecular docking studies with tomatinase', Natural product research, vol. 34, no. 18, pp. 2642-2646. https://doi.org/10.1080/14786419.2018.1548447
Zulqarnain, Iqbal Z, Cox R, Anwar J, Ahmad N, Khan K et al. Antifungal activity of compounds isolated from Aspergillus niger and their molecular docking studies with tomatinase. Natural product research. 2020 Sept 16;34(18):2642-2646. Epub 2018 Dec 24. doi: 10.1080/14786419.2018.1548447
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abstract = "Using a dual culture antagonism assay, Aspergillus niger exhibited 51.5 ± 1.1% growth inhibition against Fusarium oxysporum f.sp. lycopersici, the wilt pathogen of tomato. For enhanced production of antifungal metabolites, nutrient optimization was performed and in vitro well-diffusion antifungal assays demonstrated that crude extract obtained from GPYB culture showed a maximum zone of inhibition (8.8 ± 0.4 mm) against the wilt pathogen, which is corroborated by the comparative LCMS profiles of the extracts from all three media i.e. GPYB, YEB and PDB. Two known compounds, Asperazine (m/z 665 [M + H]+) and Nigerone (m/z 571 [M + H]+), were isolated from A. niger and their antifungal activity is reported here for the first time. In MIC experiments, Asperazine and Nigerone inhibited the pathogen at 60 and 80 µg·mL−1 respectively. Molecular docking studies of Nigerone and Asperazine with F. oxysporum tomatinase showed five and six binding interactions respectively.",
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