Details
Original language | German |
---|---|
Qualification | Doctor rerum naturalium |
Awarding Institution | |
Supervised by |
|
Date of Award | 15 Dec 2009 |
Publication status | Published - 2009 |
Abstract
In the field of tissue engineering, there is a demand to create functioning three-dimensional tissues and cell-coated scaffolds that shall be transplanted to improve tissue regeneration. The two-photon polymerization technique enables the design of any desired three-dimensional scaffold composed of photosensitive materials. In dependence of size and structure dimensions cells either fell within the features, lay on the top or adhered on lateral surfaces. To generate tissues, cells were transported and arranged in defined patterns by laser induced forward transfer method. This procedure did not harm the cells with respect to DNA strand breaks and proliferation.
Implants of the next generation shall not only be biocompatible, but also selectively guide and control cellular behavior to improve implant adaptation. It was shown that not only material hydrophobicity, but also surface topographies, which were applied for material functionalization, induced a selective cell control. Various and controllable surface features such as groove and spike structures in micrometer scale, lotusstructures and diverse nanostructures were fabricated via ablation with femtosecond lasers or negative replication process. It was observed that structuring always changed surface wettability and decreased the surface area for contact. Fibroblasts, a cell type of interest since it participates in the formation of scare tissues and therefore, reduces implant functions, were always inhibited on the provided topographies and hydrophobic materials, whereas neuroblastoma cells, endothelial cells and osteoblasts were not negatively affected.
With respect to the results and the fact that adhesion pattern and kinetic were cell specific, it was hypothezied that the selective cell control of materials is caused by cell specific differences in adhesion mechanism. For this purpose, the influence of adhesion ligands on cellular behavior was investigated. It was found that the cells respond to all ligands with a cell specific priority ranking. Furthermore, cell behavior was dependent on ligand concentration. These findings explain the observed results and facilitate the material search for future biomedical applications.
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
2009. 156 p.
Research output: Thesis › Doctoral thesis
}
TY - BOOK
T1 - Analysis of cell specific responses to polymers, hydrogels, metals, adhesion ligands, laser-fabricated three-dimensional scaffolds, and topographically-functionalized materials for biomedical applications
AU - Schlie, Sabrina
PY - 2009
Y1 - 2009
N2 - In the field of biomedicine and tissue engineering the interactions between cells and biomaterials are of great importance. In this work, the possible biomedical use of various materials such as polymers, hydrogels, and metals in dependence of material properties and compositions was analyzed. Biomaterial-cell interactions were classified with focus on DNA damage effects, adhesion, morphology, orientation and proliferation. To characterize selective responses, the measure-ments were performed with ten different cell types.In the field of tissue engineering, there is a demand to create functioning three-dimensional tissues and cell-coated scaffolds that shall be transplanted to improve tissue regeneration. The two-photon polymerization technique enables the design of any desired three-dimensional scaffold composed of photosensitive materials. In dependence of size and structure dimensions cells either fell within the features, lay on the top or adhered on lateral surfaces. To generate tissues, cells were transported and arranged in defined patterns by laser induced forward transfer method. This procedure did not harm the cells with respect to DNA strand breaks and proliferation.Implants of the next generation shall not only be biocompatible, but also selectively guide and control cellular behavior to improve implant adaptation. It was shown that not only material hydrophobicity, but also surface topographies, which were applied for material functionalization, induced a selective cell control. Various and controllable surface features such as groove and spike structures in micrometer scale, lotusstructures and diverse nanostructures were fabricated via ablation with femtosecond lasers or negative replication process. It was observed that structuring always changed surface wettability and decreased the surface area for contact. Fibroblasts, a cell type of interest since it participates in the formation of scare tissues and therefore, reduces implant functions, were always inhibited on the provided topographies and hydrophobic materials, whereas neuroblastoma cells, endothelial cells and osteoblasts were not negatively affected.With respect to the results and the fact that adhesion pattern and kinetic were cell specific, it was hypothezied that the selective cell control of materials is caused by cell specific differences in adhesion mechanism. For this purpose, the influence of adhesion ligands on cellular behavior was investigated. It was found that the cells respond to all ligands with a cell specific priority ranking. Furthermore, cell behavior was dependent on ligand concentration. These findings explain the observed results and facilitate the material search for future biomedical applications.
AB - In the field of biomedicine and tissue engineering the interactions between cells and biomaterials are of great importance. In this work, the possible biomedical use of various materials such as polymers, hydrogels, and metals in dependence of material properties and compositions was analyzed. Biomaterial-cell interactions were classified with focus on DNA damage effects, adhesion, morphology, orientation and proliferation. To characterize selective responses, the measure-ments were performed with ten different cell types.In the field of tissue engineering, there is a demand to create functioning three-dimensional tissues and cell-coated scaffolds that shall be transplanted to improve tissue regeneration. The two-photon polymerization technique enables the design of any desired three-dimensional scaffold composed of photosensitive materials. In dependence of size and structure dimensions cells either fell within the features, lay on the top or adhered on lateral surfaces. To generate tissues, cells were transported and arranged in defined patterns by laser induced forward transfer method. This procedure did not harm the cells with respect to DNA strand breaks and proliferation.Implants of the next generation shall not only be biocompatible, but also selectively guide and control cellular behavior to improve implant adaptation. It was shown that not only material hydrophobicity, but also surface topographies, which were applied for material functionalization, induced a selective cell control. Various and controllable surface features such as groove and spike structures in micrometer scale, lotusstructures and diverse nanostructures were fabricated via ablation with femtosecond lasers or negative replication process. It was observed that structuring always changed surface wettability and decreased the surface area for contact. Fibroblasts, a cell type of interest since it participates in the formation of scare tissues and therefore, reduces implant functions, were always inhibited on the provided topographies and hydrophobic materials, whereas neuroblastoma cells, endothelial cells and osteoblasts were not negatively affected.With respect to the results and the fact that adhesion pattern and kinetic were cell specific, it was hypothezied that the selective cell control of materials is caused by cell specific differences in adhesion mechanism. For this purpose, the influence of adhesion ligands on cellular behavior was investigated. It was found that the cells respond to all ligands with a cell specific priority ranking. Furthermore, cell behavior was dependent on ligand concentration. These findings explain the observed results and facilitate the material search for future biomedical applications.
U2 - 10.15488/7299
DO - 10.15488/7299
M3 - Dissertation
ER -