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A Simple Method for the Isolation and Purification of L-Asparaginase from Enterobacter aerogenes

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Original languageEnglish
Pages (from-to)15-18
Number of pages4
JournalFolia microbiologica
Volume44
Issue number1
Publication statusPublished - Feb 1999

Abstract

L-Asparaginase from Enterobacter aerogenes was purified by a simple method involving sonication of the crude cell mass, gel filtration with Sephacryl S-100 as the separating material, followed by ultrafiltration. Recent methods involve complex purification procedures of 5-6 steps. The isolation process resulted in 10-fold purification of the enzyme with a specific activity of 55 IU/mg protein and recovery of 54 %. The purity was tested by capillary electrophoresis, used for the first time for documenting the purification of L-asparaginase. The choice of the column material was critical in the purification process.

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A Simple Method for the Isolation and Purification of L-Asparaginase from Enterobacter aerogenes. / Mukherjee, J.; Joeris, K.; Riechel, P. et al.
In: Folia microbiologica, Vol. 44, No. 1, 02.1999, p. 15-18.

Research output: Contribution to journalArticleResearchpeer review

Mukherjee, J. ; Joeris, K. ; Riechel, P. et al. / A Simple Method for the Isolation and Purification of L-Asparaginase from Enterobacter aerogenes. In: Folia microbiologica. 1999 ; Vol. 44, No. 1. pp. 15-18.
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