Details
Original language | English |
---|---|
Pages (from-to) | 15-18 |
Number of pages | 4 |
Journal | Folia microbiologica |
Volume | 44 |
Issue number | 1 |
Publication status | Published - Feb 1999 |
Abstract
L-Asparaginase from Enterobacter aerogenes was purified by a simple method involving sonication of the crude cell mass, gel filtration with Sephacryl S-100 as the separating material, followed by ultrafiltration. Recent methods involve complex purification procedures of 5-6 steps. The isolation process resulted in 10-fold purification of the enzyme with a specific activity of 55 IU/mg protein and recovery of 54 %. The purity was tested by capillary electrophoresis, used for the first time for documenting the purification of L-asparaginase. The choice of the column material was critical in the purification process.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
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In: Folia microbiologica, Vol. 44, No. 1, 02.1999, p. 15-18.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A Simple Method for the Isolation and Purification of L-Asparaginase from Enterobacter aerogenes
AU - Mukherjee, J.
AU - Joeris, K.
AU - Riechel, P.
AU - Scheper, T.
N1 - Funding information: The first author wishes to thank the Deutscher Akademischer Austauschdienst and the Gottlieb Daimler und Karl Benz Stifiung for their financial support.
PY - 1999/2
Y1 - 1999/2
N2 - L-Asparaginase from Enterobacter aerogenes was purified by a simple method involving sonication of the crude cell mass, gel filtration with Sephacryl S-100 as the separating material, followed by ultrafiltration. Recent methods involve complex purification procedures of 5-6 steps. The isolation process resulted in 10-fold purification of the enzyme with a specific activity of 55 IU/mg protein and recovery of 54 %. The purity was tested by capillary electrophoresis, used for the first time for documenting the purification of L-asparaginase. The choice of the column material was critical in the purification process.
AB - L-Asparaginase from Enterobacter aerogenes was purified by a simple method involving sonication of the crude cell mass, gel filtration with Sephacryl S-100 as the separating material, followed by ultrafiltration. Recent methods involve complex purification procedures of 5-6 steps. The isolation process resulted in 10-fold purification of the enzyme with a specific activity of 55 IU/mg protein and recovery of 54 %. The purity was tested by capillary electrophoresis, used for the first time for documenting the purification of L-asparaginase. The choice of the column material was critical in the purification process.
UR - http://www.scopus.com/inward/record.url?scp=0032616756&partnerID=8YFLogxK
U2 - 10.1007/BF02816214
DO - 10.1007/BF02816214
M3 - Article
C2 - 10489691
AN - SCOPUS:0032616756
VL - 44
SP - 15
EP - 18
JO - Folia microbiologica
JF - Folia microbiologica
SN - 0015-5632
IS - 1
ER -