TY - JOUR

T1 - Varying the sustained release of BMP-2 from chitosan nanogel-functionalized polycaprolactone fiber mats by different polycaprolactone surface modifications

AU - Sundermann, Julius

AU - Oehmichen, Sarah

AU - Sydow, Steffen

AU - Burmeister, Laura

AU - Quaas, Bastian

AU - Hänsch, Robert

AU - Rinas, Ursula

AU - Hoffmann, Andrea

AU - Menzel, Henning

AU - Bunjes, Heike

N1 - Funding Information: We thank the Deutsche Forschungsgemeinschaft for funding within the research unit “Gradierte Implantate” (FOR 2180), the Ministry of Science and Culture (MWK) of Lower Saxony, Germany, for the financial support within the Smart BioTecs alliance, Alexander Becker and colleagues at the Institute for Multiphase Processes, Leibniz Universität Hannover, for the supply of electrospun batch fiber mats and Stefanie Dirksen‐Thedens for her experimental support.

PY - 2021/5/1

Y1 - 2021/5/1

N2 - Polycaprolactone (PCL) fiber mats with different surface modifications were functionalized with a chitosan nanogel coating to attach the growth factor human bone morphogenetic protein 2 (BMP-2). Three different hydrophilic surface modifications were compared with regard to the binding and in vitro release of BMP-2. The type of surface modification and the specific surface area derived from the fiber thickness had an important influence on the degree of protein loading. Coating the PCL fibers with polydopamine resulted in the binding of the largest BMP-2 quantity per surface area. However, most of the binding was irreversible over the investigated period of time, causing a low release in vitro. PCL fiber mats with a chitosan-graft-PCL coating and an additional alginate layer, as well as PCL fiber mats with an air plasma surface modification boundless BMP-2, but the immobilized protein could almost completely be released. With polydopamine and plasma modifications as well as with unmodified PCL, high amounts of BMP-2 could also be attached directly to the surface. Integration of BMP-2 into the chitosan nanogel functionalization considerably increased binding on all hydrophilized surfaces and resulted in a sustained release with an initial burst release of BMP-2 without detectable loss of bioactivity in vitro.

AB - Polycaprolactone (PCL) fiber mats with different surface modifications were functionalized with a chitosan nanogel coating to attach the growth factor human bone morphogenetic protein 2 (BMP-2). Three different hydrophilic surface modifications were compared with regard to the binding and in vitro release of BMP-2. The type of surface modification and the specific surface area derived from the fiber thickness had an important influence on the degree of protein loading. Coating the PCL fibers with polydopamine resulted in the binding of the largest BMP-2 quantity per surface area. However, most of the binding was irreversible over the investigated period of time, causing a low release in vitro. PCL fiber mats with a chitosan-graft-PCL coating and an additional alginate layer, as well as PCL fiber mats with an air plasma surface modification boundless BMP-2, but the immobilized protein could almost completely be released. With polydopamine and plasma modifications as well as with unmodified PCL, high amounts of BMP-2 could also be attached directly to the surface. Integration of BMP-2 into the chitosan nanogel functionalization considerably increased binding on all hydrophilized surfaces and resulted in a sustained release with an initial burst release of BMP-2 without detectable loss of bioactivity in vitro.

KW - BMP-2

KW - chitosan coating

KW - growth factor delivery

KW - PCL fiber scaffold

KW - surface modification

UR - http://www.scopus.com/inward/record.url?scp=85087782238&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.37045

DO - 10.1002/jbm.a.37045

M3 - Article

C2 - 32608183

AN - SCOPUS:85087782238

VL - 109

SP - 600

EP - 614

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 5

ER -