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The same but different: multiple functions of the fungal flavin dependent monooxygenase SorD fromPenicillium chrysogenum

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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OriginalspracheEnglisch
Seiten (von - bis)10934-10937
Seitenumfang4
FachzeitschriftChemical communications
Jahrgang56
Ausgabenummer74
PublikationsstatusVeröffentlicht - 21 Sept. 2020

Abstract

Sorbicillinoids are a large family of fungal secondary metabolites with a diverse range of structures and numerous bioactivites, some of which have pharmaceutical potential. The flavin-dependent monooxygenase SorD fromPenicillium chrysogenum(PcSorD) utilizes sorbicillinol to catalyze a broad scope of reactions: formation of oxosorbicillinol and epoxysorbicillinol; intermolecular Diels-Alder and Michael-addition dimerization reactions; and dimerization of a sorbicillinol derivative with oxosorbicillinol. PcSorD shares only 18.3% sequence identity with SorD fromTrichoderma reesei(TrSorD) and yet unexpectedly catalyzes many of the same reactions, however, the formation of oxosorbicillinol and bisvertinolone by PcSorD extends the range of reactions catalyzed by a single enzyme. Phylogenetic analysis indicates that PcSorD and TrSorD bind the flavin cofactor covalently butviadifferent residues and point mutations confirm these residues are essential for activity.

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The same but different: multiple functions of the fungal flavin dependent monooxygenase SorD fromPenicillium chrysogenum. / Kahlert, Lukas; Cox, Russell J.; Skellam, Elizabeth.
in: Chemical communications, Jahrgang 56, Nr. 74, 21.09.2020, S. 10934-10937.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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AU - Kahlert, Lukas

AU - Cox, Russell J.

AU - Skellam, Elizabeth

N1 - Funding Information: This work was supported by the German Research Foundation (DFG, INST 187/621-1, INST 187/686-1). LK was funded by DFG (CO 1328/5-1).

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Y1 - 2020/9/21

N2 - Sorbicillinoids are a large family of fungal secondary metabolites with a diverse range of structures and numerous bioactivites, some of which have pharmaceutical potential. The flavin-dependent monooxygenase SorD fromPenicillium chrysogenum(PcSorD) utilizes sorbicillinol to catalyze a broad scope of reactions: formation of oxosorbicillinol and epoxysorbicillinol; intermolecular Diels-Alder and Michael-addition dimerization reactions; and dimerization of a sorbicillinol derivative with oxosorbicillinol. PcSorD shares only 18.3% sequence identity with SorD fromTrichoderma reesei(TrSorD) and yet unexpectedly catalyzes many of the same reactions, however, the formation of oxosorbicillinol and bisvertinolone by PcSorD extends the range of reactions catalyzed by a single enzyme. Phylogenetic analysis indicates that PcSorD and TrSorD bind the flavin cofactor covalently butviadifferent residues and point mutations confirm these residues are essential for activity.

AB - Sorbicillinoids are a large family of fungal secondary metabolites with a diverse range of structures and numerous bioactivites, some of which have pharmaceutical potential. The flavin-dependent monooxygenase SorD fromPenicillium chrysogenum(PcSorD) utilizes sorbicillinol to catalyze a broad scope of reactions: formation of oxosorbicillinol and epoxysorbicillinol; intermolecular Diels-Alder and Michael-addition dimerization reactions; and dimerization of a sorbicillinol derivative with oxosorbicillinol. PcSorD shares only 18.3% sequence identity with SorD fromTrichoderma reesei(TrSorD) and yet unexpectedly catalyzes many of the same reactions, however, the formation of oxosorbicillinol and bisvertinolone by PcSorD extends the range of reactions catalyzed by a single enzyme. Phylogenetic analysis indicates that PcSorD and TrSorD bind the flavin cofactor covalently butviadifferent residues and point mutations confirm these residues are essential for activity.

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