Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 523-530 |
| Seitenumfang | 8 |
| Fachzeitschrift | BBA - General Subjects |
| Jahrgang | 883 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 1 Okt. 1986 |
| Extern publiziert | Ja |
Abstract
A vigorous formation of the volatile C6 compounds typical of plant lipoxygenase/hydroperoxide lyase systems (n-hexanal and hexenals) was observed in mechanically disrupted tissue of quick grass (Agropyron repens). Evidence for the precursor properties of linolenic acid and for the primary main cleavage product (Z)-3-hexenal was obtained from time-course studies. Both the lipoxygenase and the hydroperoxide lyase activities occur in a pellet sedimenting at 15 000 × g. The relative proportions of 13- to 9-hydroperoxi-(E,Z)-10,12-octadecadienoic acids were 81 to 19 under the conditions used. The enantiomeric composition of the 13-hydroperoxide, the sole substrate for the lyase, was approx. 74 to 26 (13S to 13R) and remained unchanged during prolonged incubation. The contribution of lytic activities to the formation of 1-penten-3-ol is suggested. The limited activity of the hexenal-forming system was neither caused by the carbonyls themselves nor by a hydroperoxide deficiency, and the inactivation followed third-order reaction kinetics.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biophysik
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
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in: BBA - General Subjects, Jahrgang 883, Nr. 3, 01.10.1986, S. 523-530.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - The C6-aldehyde-forming system in Agropyron repens
AU - Berger, Ralf G.
AU - Kler, Adolf
AU - Drawert, Friedrich
N1 - Funding information: The project was supported by the BMFT (Federal Ministry of Science and Technology), Bonn. S. Nitz and H. Pivernetza ssistedin mass spectrometryW. e are gratefult o G. Leupold for supplyingc apillaryc olumns.
PY - 1986/10/1
Y1 - 1986/10/1
N2 - A vigorous formation of the volatile C6 compounds typical of plant lipoxygenase/hydroperoxide lyase systems (n-hexanal and hexenals) was observed in mechanically disrupted tissue of quick grass (Agropyron repens). Evidence for the precursor properties of linolenic acid and for the primary main cleavage product (Z)-3-hexenal was obtained from time-course studies. Both the lipoxygenase and the hydroperoxide lyase activities occur in a pellet sedimenting at 15 000 × g. The relative proportions of 13- to 9-hydroperoxi-(E,Z)-10,12-octadecadienoic acids were 81 to 19 under the conditions used. The enantiomeric composition of the 13-hydroperoxide, the sole substrate for the lyase, was approx. 74 to 26 (13S to 13R) and remained unchanged during prolonged incubation. The contribution of lytic activities to the formation of 1-penten-3-ol is suggested. The limited activity of the hexenal-forming system was neither caused by the carbonyls themselves nor by a hydroperoxide deficiency, and the inactivation followed third-order reaction kinetics.
AB - A vigorous formation of the volatile C6 compounds typical of plant lipoxygenase/hydroperoxide lyase systems (n-hexanal and hexenals) was observed in mechanically disrupted tissue of quick grass (Agropyron repens). Evidence for the precursor properties of linolenic acid and for the primary main cleavage product (Z)-3-hexenal was obtained from time-course studies. Both the lipoxygenase and the hydroperoxide lyase activities occur in a pellet sedimenting at 15 000 × g. The relative proportions of 13- to 9-hydroperoxi-(E,Z)-10,12-octadecadienoic acids were 81 to 19 under the conditions used. The enantiomeric composition of the 13-hydroperoxide, the sole substrate for the lyase, was approx. 74 to 26 (13S to 13R) and remained unchanged during prolonged incubation. The contribution of lytic activities to the formation of 1-penten-3-ol is suggested. The limited activity of the hexenal-forming system was neither caused by the carbonyls themselves nor by a hydroperoxide deficiency, and the inactivation followed third-order reaction kinetics.
KW - (A. repens)
KW - C Aldehyde
KW - Enzyme inactivation
KW - Enzyme selectivity
KW - Fatty acid hydroperoxide
KW - Hexenal
KW - Lipoxygenase
UR - http://www.scopus.com/inward/record.url?scp=0000684930&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(86)90293-X
DO - 10.1016/0304-4165(86)90293-X
M3 - Article
AN - SCOPUS:0000684930
VL - 883
SP - 523
EP - 530
JO - BBA - General Subjects
JF - BBA - General Subjects
SN - 0304-4165
IS - 3
ER -