TY - JOUR

T1 - Spray- and laser-assisted biomaterial processing for fast and efficient autologous cell-plus-matrix tissue engineering

AU - Klopsch, Christian

AU - Gäbel, Ralf

AU - Kaminski, Alexander

AU - Mark, Peter

AU - Wang, Weiwei

AU - Toelk, Anita

AU - Delyagina, Evgenya

AU - Kleiner, Gabriela

AU - Koch, Lothar

AU - Chichkov, Boris

AU - Mela, Petra

AU - Jockenhoevel, Stefan

AU - Ma, Nan

AU - Steinhoff, Gustav

N1 - Funding information: We thank Ms Margit Fritsche for excellent technical assistance. This work was supported by the German Ministry of Education (BMBF; Berlin, Germany; Grant No. 0312138 A), the Ministry of Economy (Mecklenburg–West Pomerania, Schwerin; Grant No. RTC V220-630-08-TFMV-F/S-035) and the German Research Foundation (DFG; Berlin, Germany, Grant No. SFB TR37, TPA4) and REBIRTH Cluster of Excellence (Grant No. Exc62/1).

PY - 2012/12/4

Y1 - 2012/12/4

N2 - At present, intensive investigation aims at the creation of optimal valvular prostheses. We introduced and tested the applicability and functionality of two advanced cell-plus-matrix seeding technologies, spray-assisted bioprocessing (SaBP) and laser-assisted bioprocessing (LaBP), for autologous tissue engineering (TE) of bioresorbable artificial grafts. For SaBP, human mesenchymal stem cells (HMSCs), umbilical cord vein endothelial cells (HUVECs) and fibrin were simultaneously spray-administered on poly(ε-caprolactone) (PCL) substrates. For LaBP, HUVECs and HMSCs were separately laser-printed in stripes, followed by fibrin sealing. Three-leaflet valves were manufactured following TE of electrospun PCL tissue equivalents. Grafts were monitored in vitro under static and dynamic conditions in bioreactors. SaBP and LaBP resulted in TE of grafts with homogeneous cell distribution and accurate cell pattern, respectively. The engineered valves demonstrated immediate sufficient performance, complete cell coating, proliferation, engraftment, HUVEC-mediated invasion, HMSC differentiation and extracellular matrix deposition. SaBP revealed higher efficiency, with at least 12-fold shorter processing time than the applied LaBP set-up. LaBP realized coating with higher cell density and minimal cell-scaffold distance. Fibrin and PCL stability remain issues for improvement. The introduced TE technologies resulted in complete valvular cell-plus-matrix coating, excellent engraftment and HMSCs differentiation. SaBP might have potential for intraoperative table-side TE considering the procedural duration and ease of implementation. LaBP might accelerate engraftment with precise patterns.

AB - At present, intensive investigation aims at the creation of optimal valvular prostheses. We introduced and tested the applicability and functionality of two advanced cell-plus-matrix seeding technologies, spray-assisted bioprocessing (SaBP) and laser-assisted bioprocessing (LaBP), for autologous tissue engineering (TE) of bioresorbable artificial grafts. For SaBP, human mesenchymal stem cells (HMSCs), umbilical cord vein endothelial cells (HUVECs) and fibrin were simultaneously spray-administered on poly(ε-caprolactone) (PCL) substrates. For LaBP, HUVECs and HMSCs were separately laser-printed in stripes, followed by fibrin sealing. Three-leaflet valves were manufactured following TE of electrospun PCL tissue equivalents. Grafts were monitored in vitro under static and dynamic conditions in bioreactors. SaBP and LaBP resulted in TE of grafts with homogeneous cell distribution and accurate cell pattern, respectively. The engineered valves demonstrated immediate sufficient performance, complete cell coating, proliferation, engraftment, HUVEC-mediated invasion, HMSC differentiation and extracellular matrix deposition. SaBP revealed higher efficiency, with at least 12-fold shorter processing time than the applied LaBP set-up. LaBP realized coating with higher cell density and minimal cell-scaffold distance. Fibrin and PCL stability remain issues for improvement. The introduced TE technologies resulted in complete valvular cell-plus-matrix coating, excellent engraftment and HMSCs differentiation. SaBP might have potential for intraoperative table-side TE considering the procedural duration and ease of implementation. LaBP might accelerate engraftment with precise patterns.

KW - Cell printing

KW - Heart valve

KW - Intraoperative table-side tissue engineering

KW - Laser-induced forward transfer

KW - Sprayed cell seeding

KW - Stem cell-plus-fibrin coating

UR - http://www.scopus.com/inward/record.url?scp=84955188572&partnerID=8YFLogxK

U2 - 10.1002/term.1657

DO - 10.1002/term.1657

M3 - Article

C2 - 23208947

AN - SCOPUS:84955188572

VL - 9

SP - E177-E190

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

IS - 12

ER -