Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1688-1699 |
| Seitenumfang | 12 |
| Fachzeitschrift | The ISME journal |
| Jahrgang | 13 |
| Ausgabenummer | 7 |
| Frühes Online-Datum | 4 März 2019 |
| Publikationsstatus | Veröffentlicht - Juli 2019 |
Abstract
Nitrous acid (HONO) is a precursor of the hydroxyl radical (OH), a key oxidant in the degradation of most air pollutants. Field measurements indicate a large unknown source of HONO during the day time. Release of nitrous acid (HONO) from soil has been suggested as a major source of atmospheric HONO. We hypothesize that nitrite produced by biological nitrate reduction in oxygen-limited microzones in wet soils is a source of such HONO. Indeed, we found that various contrasting soil samples emitted HONO at high water-holding capacity (75–140%), demonstrating this to be a widespread phenomenon. Supplemental nitrate stimulated HONO emissions, whereas ethanol (70% v/v) treatment to minimize microbial activities reduced HONO emissions by 80%, suggesting that nitrate-dependent biotic processes are the sources of HONO. High-throughput Illumina sequencing of 16S rRNA as well as functional gene transcripts associated with nitrate and nitrite reduction indicated that HONO emissions from soil samples were associated with nitrate reduction activities of diverse Proteobacteria. Incubation of pure cultures of bacterial nitrate reducers and gene-expression analyses, as well as the analyses of mutant strains deficient in nitrite reductases, showed positive correlations of HONO emissions with the capability of microbes to reduce nitrate to nitrite. Thus, we suggest biological nitrate reduction in oxygen-limited microzones as a hitherto unknown source of atmospheric HONO, affecting biogeochemical nitrogen cycling, atmospheric chemistry, and global modeling.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Mikrobiologie
- Agrar- und Biowissenschaften (insg.)
- Ökologie, Evolution, Verhaltenswissenschaften und Systematik
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in: The ISME journal, Jahrgang 13, Nr. 7, 07.2019, S. 1688-1699.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Soil HONO emissions at high moisture content are driven by microbial nitrate reduction to nitrite: tackling the HONO puzzle
AU - Wu, Dianming
AU - Horn, Marcus A.
AU - Behrendt, Thomas
AU - Müller, Stefan
AU - Li, Jingsong
AU - Cole, Jeff A.
AU - Xie, Baohua
AU - Ju, Xiaotang
AU - Li, Guo
AU - Ermel, Michael
AU - Oswald, Robert
AU - Fröhlich-Nowoisky, Janine
AU - Hoor, Peter
AU - Hu, Chunsheng
AU - Liu, Min
AU - Andreae, Meinrat O.
AU - Pöschl, Ulrich
AU - Cheng, Yafang
AU - Su, Hang
AU - Trebs, Ivonne
AU - Weber, Bettina
AU - Sörgel, Matthias
N1 - Funding information: We thank J. Cui and M. Badawy for helping to collect the soil samples. We also thank G. Braker and L.R. Bakken for providing pure bacteria cultures. We are grateful to J. Kesselmeier, F. X. Meixner, and H.L. Drake for allowing us to complete experiments in their laboratories. We are also grateful to M. Welling, D. Plake, U. Parchatka, A. Moravek, A. Dallinger, S. Hetz, M. Morawe, N. Roßbach, R. Mertel, and A. Wieczorek for supporting techniques in our experiments. This work was supported by the Max Planck Society and the Chinese Academy of Sciences. D. Wu was sponsored by the National Natural Science Foundation of China (41807449), Shanghai Pujiang Program (18PJ1403500), and “the Fundamental Research Funds for the Central Universities”. M. Liu was supported by the National Natural Science Foundation of China (41761144062). B. Weber would like to thank Paul Crutzen for awarding her a Nobel Laureate Fellowship (2013–2015).
PY - 2019/7
Y1 - 2019/7
N2 - Nitrous acid (HONO) is a precursor of the hydroxyl radical (OH), a key oxidant in the degradation of most air pollutants. Field measurements indicate a large unknown source of HONO during the day time. Release of nitrous acid (HONO) from soil has been suggested as a major source of atmospheric HONO. We hypothesize that nitrite produced by biological nitrate reduction in oxygen-limited microzones in wet soils is a source of such HONO. Indeed, we found that various contrasting soil samples emitted HONO at high water-holding capacity (75–140%), demonstrating this to be a widespread phenomenon. Supplemental nitrate stimulated HONO emissions, whereas ethanol (70% v/v) treatment to minimize microbial activities reduced HONO emissions by 80%, suggesting that nitrate-dependent biotic processes are the sources of HONO. High-throughput Illumina sequencing of 16S rRNA as well as functional gene transcripts associated with nitrate and nitrite reduction indicated that HONO emissions from soil samples were associated with nitrate reduction activities of diverse Proteobacteria. Incubation of pure cultures of bacterial nitrate reducers and gene-expression analyses, as well as the analyses of mutant strains deficient in nitrite reductases, showed positive correlations of HONO emissions with the capability of microbes to reduce nitrate to nitrite. Thus, we suggest biological nitrate reduction in oxygen-limited microzones as a hitherto unknown source of atmospheric HONO, affecting biogeochemical nitrogen cycling, atmospheric chemistry, and global modeling.
AB - Nitrous acid (HONO) is a precursor of the hydroxyl radical (OH), a key oxidant in the degradation of most air pollutants. Field measurements indicate a large unknown source of HONO during the day time. Release of nitrous acid (HONO) from soil has been suggested as a major source of atmospheric HONO. We hypothesize that nitrite produced by biological nitrate reduction in oxygen-limited microzones in wet soils is a source of such HONO. Indeed, we found that various contrasting soil samples emitted HONO at high water-holding capacity (75–140%), demonstrating this to be a widespread phenomenon. Supplemental nitrate stimulated HONO emissions, whereas ethanol (70% v/v) treatment to minimize microbial activities reduced HONO emissions by 80%, suggesting that nitrate-dependent biotic processes are the sources of HONO. High-throughput Illumina sequencing of 16S rRNA as well as functional gene transcripts associated with nitrate and nitrite reduction indicated that HONO emissions from soil samples were associated with nitrate reduction activities of diverse Proteobacteria. Incubation of pure cultures of bacterial nitrate reducers and gene-expression analyses, as well as the analyses of mutant strains deficient in nitrite reductases, showed positive correlations of HONO emissions with the capability of microbes to reduce nitrate to nitrite. Thus, we suggest biological nitrate reduction in oxygen-limited microzones as a hitherto unknown source of atmospheric HONO, affecting biogeochemical nitrogen cycling, atmospheric chemistry, and global modeling.
UR - http://www.scopus.com/inward/record.url?scp=85062476001&partnerID=8YFLogxK
U2 - 10.15488/15944
DO - 10.15488/15944
M3 - Article
VL - 13
SP - 1688
EP - 1699
JO - The ISME journal
JF - The ISME journal
SN - 1751-7362
IS - 7
ER -