Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1204-1210 |
| Seitenumfang | 7 |
| Fachzeitschrift | Macromolecular Rapid Communications |
| Jahrgang | 35 |
| Ausgabenummer | 13 |
| Publikationsstatus | Veröffentlicht - Juli 2014 |
Abstract
A surface plasmon resonance (SPR) expression after hybridization of chitosan-gold nanoparticle-antibody (CS-AuNPs-Ab) based on: i) metal-free click chemistry, and, ii) in water system as an approach for a rapid antigen sensing, is proposed. The chitosan-hydroxybenzyl triazole complex enables us to carry out the conjugation of mPEG and trifluoromethylated oxanorbornadiene (OND) in water. CS-mPEG-OND further allows metal-free click to hybridize chitosan (CS) with azido-modified gold nanoparticles (azido-AuNPs) in aqueous solution at room temperature. The CS-mPEG-OND conjugated with LipL32 antibody (Ab) not only effectively binds with LipL32 antigen (Ag) but also performs the cycloaddition with azido-AuNPs to display a change in color within 2 min. The phenomenon leads to a simple and efficient naked-eye antigen detection technique.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Organische Chemie
- Werkstoffwissenschaften (insg.)
- Polymere und Kunststoffe
- Werkstoffwissenschaften (insg.)
- Werkstoffchemie
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Macromolecular Rapid Communications, Jahrgang 35, Nr. 13, 07.2014, S. 1204-1210.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Rapid hybridization of chitosan-gold-antibodies via metal-free click in water-based systems
T2 - A model approach for naked-eye detectable antigen sensors
AU - Jirawutthiwongchai, Jatesuda
AU - Draeger, Gerald
AU - Chirachanchai, Suwabun
N1 - Copyright: Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2014/7
Y1 - 2014/7
N2 - A surface plasmon resonance (SPR) expression after hybridization of chitosan-gold nanoparticle-antibody (CS-AuNPs-Ab) based on: i) metal-free click chemistry, and, ii) in water system as an approach for a rapid antigen sensing, is proposed. The chitosan-hydroxybenzyl triazole complex enables us to carry out the conjugation of mPEG and trifluoromethylated oxanorbornadiene (OND) in water. CS-mPEG-OND further allows metal-free click to hybridize chitosan (CS) with azido-modified gold nanoparticles (azido-AuNPs) in aqueous solution at room temperature. The CS-mPEG-OND conjugated with LipL32 antibody (Ab) not only effectively binds with LipL32 antigen (Ag) but also performs the cycloaddition with azido-AuNPs to display a change in color within 2 min. The phenomenon leads to a simple and efficient naked-eye antigen detection technique.
AB - A surface plasmon resonance (SPR) expression after hybridization of chitosan-gold nanoparticle-antibody (CS-AuNPs-Ab) based on: i) metal-free click chemistry, and, ii) in water system as an approach for a rapid antigen sensing, is proposed. The chitosan-hydroxybenzyl triazole complex enables us to carry out the conjugation of mPEG and trifluoromethylated oxanorbornadiene (OND) in water. CS-mPEG-OND further allows metal-free click to hybridize chitosan (CS) with azido-modified gold nanoparticles (azido-AuNPs) in aqueous solution at room temperature. The CS-mPEG-OND conjugated with LipL32 antibody (Ab) not only effectively binds with LipL32 antigen (Ag) but also performs the cycloaddition with azido-AuNPs to display a change in color within 2 min. The phenomenon leads to a simple and efficient naked-eye antigen detection technique.
KW - antigen sensors
KW - gold nanoparticles
KW - metal-free click chemistry
KW - oxanorbornadiene
KW - water soluble chitosan
UR - http://www.scopus.com/inward/record.url?scp=84903775694&partnerID=8YFLogxK
U2 - 10.1002/marc.201400092
DO - 10.1002/marc.201400092
M3 - Article
C2 - 24729187
AN - SCOPUS:84903775694
VL - 35
SP - 1204
EP - 1210
JO - Macromolecular Rapid Communications
JF - Macromolecular Rapid Communications
SN - 1022-1336
IS - 13
ER -