Raman-based analysis and structural differentiation of potentially harmful algae and cyanobacteria

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OriginalspracheEnglisch
Titel des SammelwerksImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI
Herausgeber/-innenAttila Tarnok, Jessica P. Houston
Herausgeber (Verlag)SPIE
ISBN (elektronisch)9781510658714
PublikationsstatusVeröffentlicht - 15 März 2023
VeranstaltungImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023 - San Francisco, USA / Vereinigte Staaten
Dauer: 30 Jan. 20231 Feb. 2023

Publikationsreihe

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Band12383
ISSN (Print)1605-7422

Abstract

Algal blooms appear most often during the summertime in marine or fresh water resources and can be harmful to humans, animals, and aquatic life. Especially cyanobacteria blooms can be dangerous as they are able to produce cyanotoxins, which can be harmful already at low concentrations. Consequently, it is very important to analyze algae and cyanobacteria blooms quickly, in situ and without complex sample preparation to identify whether the bloom can be harmful or not and to initiate further steps in time. Raman spectroscopy is capable of analyzing organic samples in situ and non-invasively with high specificity. It allows to investigate the structure of algae and cyanobacteria without physical contact and a time effective sample preparation is possible. In this work, we present a Raman based approach to analyze algae and cyanobacteria in the visible wavelength range. We determine components and the structure of the cells to distinguish potentially harmful species and also non-harmful species. Most of the fluorescence signal from the algae is suppressed so that the Raman signals from components inside the cell can be measured. The acquired spectra are processed to compare them and to retrieve information about the differences in the inner structure. Our Raman-based approach is thus suited for fast analysis and distinction of potentially harmful algae and cyanobacteria.

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Raman-based analysis and structural differentiation of potentially harmful algae and cyanobacteria. / Wetzel, Christoph; Roth, Bernhard.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI. Hrsg. / Attila Tarnok; Jessica P. Houston. SPIE, 2023. 123830C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 12383).

Publikation: Beitrag in Buch/Bericht/Sammelwerk/KonferenzbandAufsatz in KonferenzbandForschungPeer-Review

Wetzel, C & Roth, B 2023, Raman-based analysis and structural differentiation of potentially harmful algae and cyanobacteria. in A Tarnok & JP Houston (Hrsg.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI., 123830C, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Bd. 12383, SPIE, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023, San Francisco, USA / Vereinigte Staaten, 30 Jan. 2023. https://doi.org/10.1117/12.2648773
Wetzel, C., & Roth, B. (2023). Raman-based analysis and structural differentiation of potentially harmful algae and cyanobacteria. In A. Tarnok, & J. P. Houston (Hrsg.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI Artikel 123830C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 12383). SPIE. https://doi.org/10.1117/12.2648773
Wetzel C, Roth B. Raman-based analysis and structural differentiation of potentially harmful algae and cyanobacteria. in Tarnok A, Houston JP, Hrsg., Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI. SPIE. 2023. 123830C. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2648773
Wetzel, Christoph ; Roth, Bernhard. / Raman-based analysis and structural differentiation of potentially harmful algae and cyanobacteria. Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI. Hrsg. / Attila Tarnok ; Jessica P. Houston. SPIE, 2023. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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N2 - Algal blooms appear most often during the summertime in marine or fresh water resources and can be harmful to humans, animals, and aquatic life. Especially cyanobacteria blooms can be dangerous as they are able to produce cyanotoxins, which can be harmful already at low concentrations. Consequently, it is very important to analyze algae and cyanobacteria blooms quickly, in situ and without complex sample preparation to identify whether the bloom can be harmful or not and to initiate further steps in time. Raman spectroscopy is capable of analyzing organic samples in situ and non-invasively with high specificity. It allows to investigate the structure of algae and cyanobacteria without physical contact and a time effective sample preparation is possible. In this work, we present a Raman based approach to analyze algae and cyanobacteria in the visible wavelength range. We determine components and the structure of the cells to distinguish potentially harmful species and also non-harmful species. Most of the fluorescence signal from the algae is suppressed so that the Raman signals from components inside the cell can be measured. The acquired spectra are processed to compare them and to retrieve information about the differences in the inner structure. Our Raman-based approach is thus suited for fast analysis and distinction of potentially harmful algae and cyanobacteria.

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