TY - JOUR

T1 - Production of natural colorants by liquid fermentation with Chlorociboria aeruginascens and Laetiporus sulphureus and prospective applications

AU - Zschätzsch, Marlen

AU - Steudler, Susanne

AU - Reinhardt, Olena

AU - Bergmann, Pia

AU - Ersoy, Franziska

AU - Stange, Stephanie

AU - Wagenführ, André

AU - Walther, Thomas

AU - Berger, Ralf Günter

AU - Werner, Anett

N1 - Funding Information: This research was funded by the Federal Ministry for Economic Affairs and Energy, grant number KF 2418631 and KF 2049821 and the Federal Ministry of Education and Research, grant number 031B0879.

PY - 2021/3/2

Y1 - 2021/3/2

N2 - The replacement of potentially hazardous synthetic dyes with natural dyes and pigments are of great interest for a sustainable economy. In order to obtain cost-efficient, environmentally friendly and competitive products, improvements in the cultivation and extraction of pigment-producing organisms and in dyeing processes are necessary. In our study, we were able to scale up the production of xylindein by Chlorociboria aeruginascens from 3 to 70 L bioreactor cultivations. We have identified important bioprocess parameters like low shear stress (150 rpm, tip speed <0.5 m/s) for optimal pigment yield (4.8 mg/L/d). Additionally, we have demonstrated the potential of laetiporic acid production by Laetiporus sulphureus in various cultivation systems and media, achieving dried biomass concentrations of almost 10 g/L with a 7 L bioreactor cultivation after 17 days. Extractions performed at 70°C and 15 min incubation time showed optimal results. To the best of our knowledge, we have described for the first time the use of this pigment in silk dyeing, which results in a brilliant hue that cannot easily be produced by other natural pigments.

AB - The replacement of potentially hazardous synthetic dyes with natural dyes and pigments are of great interest for a sustainable economy. In order to obtain cost-efficient, environmentally friendly and competitive products, improvements in the cultivation and extraction of pigment-producing organisms and in dyeing processes are necessary. In our study, we were able to scale up the production of xylindein by Chlorociboria aeruginascens from 3 to 70 L bioreactor cultivations. We have identified important bioprocess parameters like low shear stress (150 rpm, tip speed <0.5 m/s) for optimal pigment yield (4.8 mg/L/d). Additionally, we have demonstrated the potential of laetiporic acid production by Laetiporus sulphureus in various cultivation systems and media, achieving dried biomass concentrations of almost 10 g/L with a 7 L bioreactor cultivation after 17 days. Extractions performed at 70°C and 15 min incubation time showed optimal results. To the best of our knowledge, we have described for the first time the use of this pigment in silk dyeing, which results in a brilliant hue that cannot easily be produced by other natural pigments.

KW - Chlorociboria aeruginascens

KW - laetiporic acid

KW - Laetiporus sulphureus

KW - natural dye

KW - xylindein

UR - http://www.scopus.com/inward/record.url?scp=85099961975&partnerID=8YFLogxK

U2 - 10.1002/elsc.202000079

DO - 10.1002/elsc.202000079

M3 - Article

C2 - 33716624

VL - 21

SP - 270

EP - 282

JO - Engineering in life sciences

JF - Engineering in life sciences

SN - 1618-0240

IS - 3-4

ER -