Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Light-Emitting Devices, Materials, and Applications XXIX |
| Herausgeber/-innen | Jong Kyu Kim, Michael R. Krames, Martin Strassburg |
| Herausgeber (Verlag) | SPIE |
| ISBN (elektronisch) | 9781510685208 |
| Publikationsstatus | Veröffentlicht - 19 März 2025 |
| Veranstaltung | SPIE Photonics West OPTO 2025 - San Francisco, United States, San Francisco, USA / Vereinigte Staaten Dauer: 25 Jan. 2025 → 31 Jan. 2025 |
Publikationsreihe
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Band | 13386 |
| ISSN (Print) | 0277-786X |
| ISSN (elektronisch) | 1996-756X |
Abstract
The principle of fluorescence is used in many areas of microscopy. In biomedicine, it can be used to observe living single cells for cancer research or antibody development. High luminous intensities are required to excite fluorescence, which is why LEDs with visible spectral range are currently used as a light source in conjunction with filters. A critical factor for efficiency of fluorescence microscopy is photobleaching of the fluorophores and phototoxicity, which leads to cell damage. Therefore, this research aims to avoid illumination of cell samples not being examined microscopically. To this end, a micro-LED array is to be developed with which parallel examinations of selected individual samples can be carried out. To enable selective illumination of cell samples a design for the optical package is needed. In addition, the number of examinations that can be carried out simultaneously should be increased. Therefore, the pitch between the samples must be small as possible, which is why the micro-LEDs’ beams must be already influenced in near field. The aim is to achieve homogeneous illumination of the cell sample under investigation with minimal edge scattering and maximal intensity. Using numerical optimization, a design for a freeform micro lens array (MLA) is created. The algorithm adapts to emitted beam shapes resulting from pixel size, pitch, and wavelength, to enable flexible adaptation of the MLA when the system parameters are changed. Finally, the package is simulated and validated using raytracing.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Physik und Astronomie (insg.)
- Physik der kondensierten Materie
- Informatik (insg.)
- Angewandte Informatik
- Mathematik (insg.)
- Angewandte Mathematik
- Ingenieurwesen (insg.)
- Elektrotechnik und Elektronik
Ziele für nachhaltige Entwicklung
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
Light-Emitting Devices, Materials, and Applications XXIX. Hrsg. / Jong Kyu Kim; Michael R. Krames; Martin Strassburg. SPIE, 2025. 133860L (Proceedings of SPIE - The International Society for Optical Engineering; Band 13386).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - Numerical optical optimization of a micro-LED package for fluorescence microscopy
AU - Fritze, Anna Lena
AU - Evertz, Andreas
AU - Leineweber, Sebastian
AU - Overmeyer, Ludger
N1 - Publisher Copyright: © 2025 SPIE.
PY - 2025/3/19
Y1 - 2025/3/19
N2 - The principle of fluorescence is used in many areas of microscopy. In biomedicine, it can be used to observe living single cells for cancer research or antibody development. High luminous intensities are required to excite fluorescence, which is why LEDs with visible spectral range are currently used as a light source in conjunction with filters. A critical factor for efficiency of fluorescence microscopy is photobleaching of the fluorophores and phototoxicity, which leads to cell damage. Therefore, this research aims to avoid illumination of cell samples not being examined microscopically. To this end, a micro-LED array is to be developed with which parallel examinations of selected individual samples can be carried out. To enable selective illumination of cell samples a design for the optical package is needed. In addition, the number of examinations that can be carried out simultaneously should be increased. Therefore, the pitch between the samples must be small as possible, which is why the micro-LEDs’ beams must be already influenced in near field. The aim is to achieve homogeneous illumination of the cell sample under investigation with minimal edge scattering and maximal intensity. Using numerical optimization, a design for a freeform micro lens array (MLA) is created. The algorithm adapts to emitted beam shapes resulting from pixel size, pitch, and wavelength, to enable flexible adaptation of the MLA when the system parameters are changed. Finally, the package is simulated and validated using raytracing.
AB - The principle of fluorescence is used in many areas of microscopy. In biomedicine, it can be used to observe living single cells for cancer research or antibody development. High luminous intensities are required to excite fluorescence, which is why LEDs with visible spectral range are currently used as a light source in conjunction with filters. A critical factor for efficiency of fluorescence microscopy is photobleaching of the fluorophores and phototoxicity, which leads to cell damage. Therefore, this research aims to avoid illumination of cell samples not being examined microscopically. To this end, a micro-LED array is to be developed with which parallel examinations of selected individual samples can be carried out. To enable selective illumination of cell samples a design for the optical package is needed. In addition, the number of examinations that can be carried out simultaneously should be increased. Therefore, the pitch between the samples must be small as possible, which is why the micro-LEDs’ beams must be already influenced in near field. The aim is to achieve homogeneous illumination of the cell sample under investigation with minimal edge scattering and maximal intensity. Using numerical optimization, a design for a freeform micro lens array (MLA) is created. The algorithm adapts to emitted beam shapes resulting from pixel size, pitch, and wavelength, to enable flexible adaptation of the MLA when the system parameters are changed. Finally, the package is simulated and validated using raytracing.
KW - freeform lens design
KW - numerical modeling
KW - optical package optimization
UR - http://www.scopus.com/inward/record.url?scp=105002721922&partnerID=8YFLogxK
U2 - 10.1117/12.3043094
DO - 10.1117/12.3043094
M3 - Conference contribution
AN - SCOPUS:105002721922
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Light-Emitting Devices, Materials, and Applications XXIX
A2 - Kim, Jong Kyu
A2 - Krames, Michael R.
A2 - Strassburg, Martin
PB - SPIE
T2 - SPIE Photonics West OPTO 2025
Y2 - 25 January 2025 through 31 January 2025
ER -