Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 534-546 |
| Seitenumfang | 13 |
| Fachzeitschrift | Dental Materials |
| Jahrgang | 37 |
| Ausgabenummer | 3 |
| Frühes Online-Datum | 23 Feb. 2021 |
| Publikationsstatus | Veröffentlicht - März 2021 |
Abstract
OBJECTIVE: The angiogenic differentiation of dental pulp stem cells (DPSCs) is important for tissue homeostasis and wound healing. In this study the influence of 2-hydroxyethyl methacrylate (HEMA) on angiogenic differentiation was investigated.
METHODS: To evaluate HEMA effects on angiogenic differentiation, DPSCs were cultivated in angiogenic differentiation medium (ADM) in the presence or absence of non-toxic HEMA concentrations (0.1 mM and 0.5 mM). Subsequently, angiogenic differentiation was analyzed on the molecular level by qRT-PCR and protein profiler analyzes of angiogenic markers and flow cytometry of PECAM1. The influence of HEMA on angiogenic phenotypes was analyzed by cell migration and sprouting assays.
RESULTS: Treatment with 0.5 mM HEMA during differentiation can lead to a slight reduction of angiogenic markers on mRNA level. HEMA also seems to slightly reduce the quantity of angiogenic cytokines (not significant). However, these HEMA concentrations have no detectable influence on cell migration, the abundance of PECAM1 and the formation of capillaries. Higher concentrations caused primary cytotoxic effects in angiogenic differentiation experiments conducted for longer periods than 72 h.
SIGNIFICANCE: Non-cytotoxic HEMA concentrations seem to have a minor impact on the expression of angiogenic markers, essentially on the mRNA level, without affecting the angiogenic differentiation process itself on a detectable level.
ASJC Scopus Sachgebiete
- Ingenieurwesen (insg.)
- Werkstoffmechanik
- Werkstoffwissenschaften (insg.)
- Zahnmedizin (insg.)
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in: Dental Materials, Jahrgang 37, Nr. 3, 03.2021, S. 534-546.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Influence of 2-hydroxyethyl methacrylate (HEMA) exposure on angiogenic differentiation of dental pulp stem cells (DPSCs)
AU - Jochums, André
AU - Volk, Joachim
AU - Perduns, Renke
AU - Plum, Melanie
AU - Schertl, Peter
AU - Bakopoulou, Athina
AU - Geurtsen, Werner
N1 - Funding Information: Our thanks are due to Ms. S. Taubeler-Gerling for her commitment and excellent technical assistance. This study was supported by a grant of the Deutsche Forschungsgemeinschaft /German National Science Foundation (DFG) ( GE 455/17-1 ).
PY - 2021/3
Y1 - 2021/3
N2 - OBJECTIVE: The angiogenic differentiation of dental pulp stem cells (DPSCs) is important for tissue homeostasis and wound healing. In this study the influence of 2-hydroxyethyl methacrylate (HEMA) on angiogenic differentiation was investigated.METHODS: To evaluate HEMA effects on angiogenic differentiation, DPSCs were cultivated in angiogenic differentiation medium (ADM) in the presence or absence of non-toxic HEMA concentrations (0.1 mM and 0.5 mM). Subsequently, angiogenic differentiation was analyzed on the molecular level by qRT-PCR and protein profiler analyzes of angiogenic markers and flow cytometry of PECAM1. The influence of HEMA on angiogenic phenotypes was analyzed by cell migration and sprouting assays.RESULTS: Treatment with 0.5 mM HEMA during differentiation can lead to a slight reduction of angiogenic markers on mRNA level. HEMA also seems to slightly reduce the quantity of angiogenic cytokines (not significant). However, these HEMA concentrations have no detectable influence on cell migration, the abundance of PECAM1 and the formation of capillaries. Higher concentrations caused primary cytotoxic effects in angiogenic differentiation experiments conducted for longer periods than 72 h.SIGNIFICANCE: Non-cytotoxic HEMA concentrations seem to have a minor impact on the expression of angiogenic markers, essentially on the mRNA level, without affecting the angiogenic differentiation process itself on a detectable level.
AB - OBJECTIVE: The angiogenic differentiation of dental pulp stem cells (DPSCs) is important for tissue homeostasis and wound healing. In this study the influence of 2-hydroxyethyl methacrylate (HEMA) on angiogenic differentiation was investigated.METHODS: To evaluate HEMA effects on angiogenic differentiation, DPSCs were cultivated in angiogenic differentiation medium (ADM) in the presence or absence of non-toxic HEMA concentrations (0.1 mM and 0.5 mM). Subsequently, angiogenic differentiation was analyzed on the molecular level by qRT-PCR and protein profiler analyzes of angiogenic markers and flow cytometry of PECAM1. The influence of HEMA on angiogenic phenotypes was analyzed by cell migration and sprouting assays.RESULTS: Treatment with 0.5 mM HEMA during differentiation can lead to a slight reduction of angiogenic markers on mRNA level. HEMA also seems to slightly reduce the quantity of angiogenic cytokines (not significant). However, these HEMA concentrations have no detectable influence on cell migration, the abundance of PECAM1 and the formation of capillaries. Higher concentrations caused primary cytotoxic effects in angiogenic differentiation experiments conducted for longer periods than 72 h.SIGNIFICANCE: Non-cytotoxic HEMA concentrations seem to have a minor impact on the expression of angiogenic markers, essentially on the mRNA level, without affecting the angiogenic differentiation process itself on a detectable level.
KW - Cell Differentiation
KW - Cells, Cultured
KW - Dental Pulp
KW - Methacrylates
KW - Stem Cells
KW - Migration
KW - Spheroid sprouting
KW - Angiogenic differentiation
KW - Dental material
KW - Gene expression
KW - Composite resin
KW - Proteome profiler
KW - HEMA
KW - Dental pulp stem cells
KW - Matrigel
UR - http://www.scopus.com/inward/record.url?scp=85100795758&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2020.12.008
DO - 10.1016/j.dental.2020.12.008
M3 - Article
C2 - 33579530
VL - 37
SP - 534
EP - 546
JO - Dental Materials
JF - Dental Materials
SN - 0109-5641
IS - 3
ER -