Genetic control of flower petal number in Rosa x Damascena Mill f. trigintipetala

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

Organisationseinheiten

Externe Organisationen

  • Rosa Select Ltd
  • Agricultural Academy
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)597-604
Seitenumfang8
FachzeitschriftBiotechnology and Biotechnological Equipment
Jahrgang33
Ausgabenummer1
Frühes Online-Datum9 Apr. 2019
PublikationsstatusElektronisch veröffentlicht (E-Pub) - 9 Apr. 2019

Abstract

We studied the petal number trait in a population obtained after self-pollination of R. x damascena f. trigintipetala following analysis of molecular markers which have previously been mapped near the major dominant locus Blfo/d6 determining this trait in other rose species including R. multiflora and R. hybrida. The results showed that the same genetic mechanism, which determines the petal number trait in R. multiflora and R. hybrida also controls the trait in R. x damascena f. trigintipetala and is related to the dominant effect of a single copy allele in the tetraploid genome of this species. We also analyzed the expression of several flower homeotic genes including R. x damascena APETALA1/FUL-like (paleo AP1 type), R. x damascena euAPETALA 3 (euAP3 line) and R. x damascena AGAMOUS in early stage flower buds corresponding to plants with double and simple flowers. The obtained results showed that only R. x damascena AGAMOUS was differentially expressed between the samples of double and simple flowers, its relative expression being upregulated 3.5-fold in simple flowers. We further cloned and sequenced the four genomic clones of R. x damascena AGAMOUS and studied the potential additive effect of this gene by analysing the segregation of its four alleles in the population of self-pollinated R. x damascena. Analysis of variance of the data for petal number and allele segregation did not show a statistically significant effect of any allele configuration of the AGAMOUS gene on the petal number trait in R. x damascena f. trigintipetala.

ASJC Scopus Sachgebiete

Zitieren

Genetic control of flower petal number in Rosa x Damascena Mill f. trigintipetala. / Rusanov, Krasimir; Kovacheva, Natasha; Rusanova, Mila et al.
in: Biotechnology and Biotechnological Equipment, Jahrgang 33, Nr. 1, 09.04.2019, S. 597-604.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Rusanov K, Kovacheva N, Rusanova M, Linde M, Debener T, Atanassov I. Genetic control of flower petal number in Rosa x Damascena Mill f. trigintipetala. Biotechnology and Biotechnological Equipment. 2019 Apr 9;33(1):597-604. Epub 2019 Apr 9. doi: 10.1080/13102818.2019.1599731, 10.15488/9835
Rusanov, Krasimir ; Kovacheva, Natasha ; Rusanova, Mila et al. / Genetic control of flower petal number in Rosa x Damascena Mill f. trigintipetala. in: Biotechnology and Biotechnological Equipment. 2019 ; Jahrgang 33, Nr. 1. S. 597-604.
Download
@article{b809a7458f5c43cca442a9febfa90b8e,
title = "Genetic control of flower petal number in Rosa x Damascena Mill f. trigintipetala",
abstract = "We studied the petal number trait in a population obtained after self-pollination of R. x damascena f. trigintipetala following analysis of molecular markers which have previously been mapped near the major dominant locus Blfo/d6 determining this trait in other rose species including R. multiflora and R. hybrida. The results showed that the same genetic mechanism, which determines the petal number trait in R. multiflora and R. hybrida also controls the trait in R. x damascena f. trigintipetala and is related to the dominant effect of a single copy allele in the tetraploid genome of this species. We also analyzed the expression of several flower homeotic genes including R. x damascena APETALA1/FUL-like (paleo AP1 type), R. x damascena euAPETALA 3 (euAP3 line) and R. x damascena AGAMOUS in early stage flower buds corresponding to plants with double and simple flowers. The obtained results showed that only R. x damascena AGAMOUS was differentially expressed between the samples of double and simple flowers, its relative expression being upregulated 3.5-fold in simple flowers. We further cloned and sequenced the four genomic clones of R. x damascena AGAMOUS and studied the potential additive effect of this gene by analysing the segregation of its four alleles in the population of self-pollinated R. x damascena. Analysis of variance of the data for petal number and allele segregation did not show a statistically significant effect of any allele configuration of the AGAMOUS gene on the petal number trait in R. x damascena f. trigintipetala.",
keywords = "AGAMOUS, number of petals, Rosa x damascena Mill f. trigintipetala",
author = "Krasimir Rusanov and Natasha Kovacheva and Mila Rusanova and Marcus Linde and Thomas Debener and Ivan Atanassov",
note = "Funding Information: This study was supported by the National Science Fund at the Ministry of Education and Science, Bulgaria, under grant DN 06/2. The authors would like to thank Marina Alexeeva (Master degree student at the Sofia University) and Rumyana Velcheva (technician at ABI) for the excellent technical assistance.",
year = "2019",
month = apr,
day = "9",
doi = "10.1080/13102818.2019.1599731",
language = "English",
volume = "33",
pages = "597--604",
journal = "Biotechnology and Biotechnological Equipment",
issn = "1310-2818",
publisher = "Taylor and Francis Ltd.",
number = "1",

}

Download

TY - JOUR

T1 - Genetic control of flower petal number in Rosa x Damascena Mill f. trigintipetala

AU - Rusanov, Krasimir

AU - Kovacheva, Natasha

AU - Rusanova, Mila

AU - Linde, Marcus

AU - Debener, Thomas

AU - Atanassov, Ivan

N1 - Funding Information: This study was supported by the National Science Fund at the Ministry of Education and Science, Bulgaria, under grant DN 06/2. The authors would like to thank Marina Alexeeva (Master degree student at the Sofia University) and Rumyana Velcheva (technician at ABI) for the excellent technical assistance.

PY - 2019/4/9

Y1 - 2019/4/9

N2 - We studied the petal number trait in a population obtained after self-pollination of R. x damascena f. trigintipetala following analysis of molecular markers which have previously been mapped near the major dominant locus Blfo/d6 determining this trait in other rose species including R. multiflora and R. hybrida. The results showed that the same genetic mechanism, which determines the petal number trait in R. multiflora and R. hybrida also controls the trait in R. x damascena f. trigintipetala and is related to the dominant effect of a single copy allele in the tetraploid genome of this species. We also analyzed the expression of several flower homeotic genes including R. x damascena APETALA1/FUL-like (paleo AP1 type), R. x damascena euAPETALA 3 (euAP3 line) and R. x damascena AGAMOUS in early stage flower buds corresponding to plants with double and simple flowers. The obtained results showed that only R. x damascena AGAMOUS was differentially expressed between the samples of double and simple flowers, its relative expression being upregulated 3.5-fold in simple flowers. We further cloned and sequenced the four genomic clones of R. x damascena AGAMOUS and studied the potential additive effect of this gene by analysing the segregation of its four alleles in the population of self-pollinated R. x damascena. Analysis of variance of the data for petal number and allele segregation did not show a statistically significant effect of any allele configuration of the AGAMOUS gene on the petal number trait in R. x damascena f. trigintipetala.

AB - We studied the petal number trait in a population obtained after self-pollination of R. x damascena f. trigintipetala following analysis of molecular markers which have previously been mapped near the major dominant locus Blfo/d6 determining this trait in other rose species including R. multiflora and R. hybrida. The results showed that the same genetic mechanism, which determines the petal number trait in R. multiflora and R. hybrida also controls the trait in R. x damascena f. trigintipetala and is related to the dominant effect of a single copy allele in the tetraploid genome of this species. We also analyzed the expression of several flower homeotic genes including R. x damascena APETALA1/FUL-like (paleo AP1 type), R. x damascena euAPETALA 3 (euAP3 line) and R. x damascena AGAMOUS in early stage flower buds corresponding to plants with double and simple flowers. The obtained results showed that only R. x damascena AGAMOUS was differentially expressed between the samples of double and simple flowers, its relative expression being upregulated 3.5-fold in simple flowers. We further cloned and sequenced the four genomic clones of R. x damascena AGAMOUS and studied the potential additive effect of this gene by analysing the segregation of its four alleles in the population of self-pollinated R. x damascena. Analysis of variance of the data for petal number and allele segregation did not show a statistically significant effect of any allele configuration of the AGAMOUS gene on the petal number trait in R. x damascena f. trigintipetala.

KW - AGAMOUS

KW - number of petals

KW - Rosa x damascena Mill f. trigintipetala

UR - http://www.scopus.com/inward/record.url?scp=85064155743&partnerID=8YFLogxK

U2 - 10.1080/13102818.2019.1599731

DO - 10.1080/13102818.2019.1599731

M3 - Article

AN - SCOPUS:85064155743

VL - 33

SP - 597

EP - 604

JO - Biotechnology and Biotechnological Equipment

JF - Biotechnology and Biotechnological Equipment

SN - 1310-2818

IS - 1

ER -

Von denselben Autoren