Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 2000107 |
| Fachzeitschrift | Macromolecular bioscience |
| Jahrgang | 20 |
| Ausgabenummer | 7 |
| Publikationsstatus | Veröffentlicht - 20 Juli 2020 |
Abstract
Many properties in both healthy and pathological tissues are highly influenced by the mechanical properties of the extracellular matrix. Stiffness gradient hydrogels are frequently used for exploring these complex relationships in mechanobiology. In this study, the fabrication of a simple, cost-efficient, and versatile system is reported for creation of stiffness gradients from photoactive hydrogels like gelatin-methacryloyl (GelMA). The setup includes syringe pumps for gradient generation and a 3D printed microfluidic device for homogenous mixing of GelMA precursors with different crosslinker concentration. The stiffness gradient is investigated by using rheology. A co-culture consisting of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) and human umbilical cord vein endothelial cells (HUVECs) is encapsulated in the gradient construct. It is possible to locate the stiffness ranges at which the studied cells displayed specific spreading morphology and migration rates. With the help of the described system, variable mechanical gradient constructs can be created and optimal 3D cell culture conditions can be experientially identified.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Werkstoffwissenschaften (insg.)
- Polymere und Kunststoffe
- Werkstoffwissenschaften (insg.)
- Werkstoffchemie
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in: Macromolecular bioscience, Jahrgang 20, Nr. 7, 2000107, 20.07.2020.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Fabrication of Stiffness Gradients of GelMA Hydrogels Using a 3D Printed Micromixer
AU - Lavrentieva, Antonina
AU - Fleischhammer, Tabea
AU - Enders, Anton
AU - Pirmahboub, Hamidreza
AU - Bahnemann, Janina
AU - Pepelanova, Iliyana
N1 - Funding information: This research was supported by the German Research Foundation (DFG Project 398007461 488 3D Dual?Gradient Systems for Functional Cell Screening) and partly funded via the DFG Emmy Noether programme, project ID 346772917. The support by the SMART BIOTECS initiative, financially supported by the Ministry of Science and Culture (MWK) of Lower Saxony, Germany is also acknowledged. The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover. The confocal laser scanning microscope was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 420505864. This research was supported by the German Research Foundation (DFG Project 398007461 488 3D Dual-Gradient Systems for Functional Cell Screening) and partly funded via the DFG Emmy Noether programme, project ID 346772917. The support by the SMART BIOTECS initiative, financially supported by the Ministry of Science and Culture (MWK) of Lower Saxony, Germany is also acknowledged. The publication of this article was funded by the Open Access Fund of Leibniz Universit?t Hannover. The confocal laser scanning microscope was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) ? 420505864.
PY - 2020/7/20
Y1 - 2020/7/20
N2 - Many properties in both healthy and pathological tissues are highly influenced by the mechanical properties of the extracellular matrix. Stiffness gradient hydrogels are frequently used for exploring these complex relationships in mechanobiology. In this study, the fabrication of a simple, cost-efficient, and versatile system is reported for creation of stiffness gradients from photoactive hydrogels like gelatin-methacryloyl (GelMA). The setup includes syringe pumps for gradient generation and a 3D printed microfluidic device for homogenous mixing of GelMA precursors with different crosslinker concentration. The stiffness gradient is investigated by using rheology. A co-culture consisting of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) and human umbilical cord vein endothelial cells (HUVECs) is encapsulated in the gradient construct. It is possible to locate the stiffness ranges at which the studied cells displayed specific spreading morphology and migration rates. With the help of the described system, variable mechanical gradient constructs can be created and optimal 3D cell culture conditions can be experientially identified.
AB - Many properties in both healthy and pathological tissues are highly influenced by the mechanical properties of the extracellular matrix. Stiffness gradient hydrogels are frequently used for exploring these complex relationships in mechanobiology. In this study, the fabrication of a simple, cost-efficient, and versatile system is reported for creation of stiffness gradients from photoactive hydrogels like gelatin-methacryloyl (GelMA). The setup includes syringe pumps for gradient generation and a 3D printed microfluidic device for homogenous mixing of GelMA precursors with different crosslinker concentration. The stiffness gradient is investigated by using rheology. A co-culture consisting of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) and human umbilical cord vein endothelial cells (HUVECs) is encapsulated in the gradient construct. It is possible to locate the stiffness ranges at which the studied cells displayed specific spreading morphology and migration rates. With the help of the described system, variable mechanical gradient constructs can be created and optimal 3D cell culture conditions can be experientially identified.
KW - 3D cell cultures
KW - 3D printing
KW - gelatin-methacryloyl hydrogel
KW - microfluidic mixers
KW - stiffness gradients
UR - http://www.scopus.com/inward/record.url?scp=85086365638&partnerID=8YFLogxK
U2 - 10.1002/mabi.202000107
DO - 10.1002/mabi.202000107
M3 - Article
C2 - 32537875
AN - SCOPUS:85086365638
VL - 20
JO - Macromolecular bioscience
JF - Macromolecular bioscience
SN - 1616-5187
IS - 7
M1 - 2000107
ER -