Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus – an unusual member of a putative new genus within the Potyviridae

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Hanna Rose
  • Ines Döring
  • Heinrich Josef Vetten
  • Wulf Menzel
  • Katja R. Richert-Pöggeler
  • Edgar Maiss

Externe Organisationen

  • Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • Julius Kühn-Institut (JKI) Bundesforschungsinstitut für Kulturpflanzen
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer001207
Seiten (von - bis)308-320
Seitenumfang13
FachzeitschriftJournal of General Virology
Jahrgang100
Ausgabenummer2
Frühes Online-Datum22 Jan. 2019
PublikationsstatusElektronisch veröffentlicht (E-Pub) - 22 Jan. 2019

Abstract

Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5¢ untranslated region (UTR) of 13 nt and a 3¢ UTR consisting of 586 nt that is not polyadenylated. CeLV RNA shares nt sequence identity of only about 40 % with other members of the Potyviridae (potyvirids). The CeLV polyprotein is notable in that it starts with a signal peptide, has a putative P3N-PIPO ORF and shares low aa sequence identity (about 18 %) with other potyvirids. Although potential cleavage sites were not identified for the N-terminal two-thirds of the polyprotein, the latter possesses a number of sequence motifs, the identity and position of which are characteristic of other potyvirids. Attempts at constructing an infectious full-length cDNA clone of CeLV were successful following Rhizobium radiobacter infiltration of Nicotiana benthamiana and Apium graveolens. CeLV appears to have the largest genome of all known potyvirids and some unique genome features that may warrant the creation of a new genus, for which we propose the name ‘celavirus’.

ASJC Scopus Sachgebiete

  • Immunologie und Mikrobiologie (insg.)
  • Virologie

Zitieren

Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus – an unusual member of a putative new genus within the Potyviridae. / Rose, Hanna; Döring, Ines; Vetten, Heinrich Josef et al.
in: Journal of General Virology, Jahrgang 100, Nr. 2, 001207, 22.01.2019, S. 308-320.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Rose H, Döring I, Vetten HJ, Menzel W, Richert-Pöggeler KR, Maiss E. Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus – an unusual member of a putative new genus within the Potyviridae. Journal of General Virology. 2019 Jan 22;100(2):308-320. 001207. Epub 2019 Jan 22. doi: 10.1099/jgv.0.001207
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title = "Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus – an unusual member of a putative new genus within the Potyviridae",
abstract = "Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5¢ untranslated region (UTR) of 13 nt and a 3¢ UTR consisting of 586 nt that is not polyadenylated. CeLV RNA shares nt sequence identity of only about 40 % with other members of the Potyviridae (potyvirids). The CeLV polyprotein is notable in that it starts with a signal peptide, has a putative P3N-PIPO ORF and shares low aa sequence identity (about 18 %) with other potyvirids. Although potential cleavage sites were not identified for the N-terminal two-thirds of the polyprotein, the latter possesses a number of sequence motifs, the identity and position of which are characteristic of other potyvirids. Attempts at constructing an infectious full-length cDNA clone of CeLV were successful following Rhizobium radiobacter infiltration of Nicotiana benthamiana and Apium graveolens. CeLV appears to have the largest genome of all known potyvirids and some unique genome features that may warrant the creation of a new genus, for which we propose the name {\textquoteleft}celavirus{\textquoteright}.",
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T1 - Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus – an unusual member of a putative new genus within the Potyviridae

AU - Rose, Hanna

AU - Döring, Ines

AU - Vetten, Heinrich Josef

AU - Menzel, Wulf

AU - Richert-Pöggeler, Katja R.

AU - Maiss, Edgar

PY - 2019/1/22

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N2 - Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5¢ untranslated region (UTR) of 13 nt and a 3¢ UTR consisting of 586 nt that is not polyadenylated. CeLV RNA shares nt sequence identity of only about 40 % with other members of the Potyviridae (potyvirids). The CeLV polyprotein is notable in that it starts with a signal peptide, has a putative P3N-PIPO ORF and shares low aa sequence identity (about 18 %) with other potyvirids. Although potential cleavage sites were not identified for the N-terminal two-thirds of the polyprotein, the latter possesses a number of sequence motifs, the identity and position of which are characteristic of other potyvirids. Attempts at constructing an infectious full-length cDNA clone of CeLV were successful following Rhizobium radiobacter infiltration of Nicotiana benthamiana and Apium graveolens. CeLV appears to have the largest genome of all known potyvirids and some unique genome features that may warrant the creation of a new genus, for which we propose the name ‘celavirus’.

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KW - Celery latent virus

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JF - Journal of General Virology

SN - 0022-1317

IS - 2

M1 - 001207

ER -

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