Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro: Is sorbitol taken up?

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)195-206
Seitenumfang12
FachzeitschriftPlant Cell, Tissue and Organ Culture
Jahrgang127
Ausgabenummer1
Frühes Online-Datum12 Juli 2016
PublikationsstatusVeröffentlicht - Okt. 2016

Abstract

Climate change and the increasing world population will lead to an increased water shortage. This gives rise to the need for plant cultivars which are drought tolerant. Solanum tuberosum L. is important not only as a nutritive rich food, but starch potatoes are of great value for the industry. Two starch genotypes of S. tuberosum L. divergently responding to osmotic stress were subjected to medium containing 0.2 M sorbitol in vitro. A targeted metabolomics approach was performed in which 42 metabolites were analysed 11 days after the transfer of the plants to the experimental medium. The sensitive genotype displayed stress responses comprising higher abundant metabolites such as phenylalanine, proline and sucrose and a decrease e.g. in GABA and fumaric acid. These can be used for protein build up, nitrogen storage and the protection through osmotic active compounds. In contrast, the tolerant genotype showed a higher abundance in compounds used as osmolytes (citric acid and proline), which might give rise to acclimatisation to the stress. Interestingly, in chromatograms of both genotypes a high sorbitol peak was detected, whereas control plants or plants treated with 4.8 % PEG 8000 did not accumulate this substance. Conclusively, sorbitol is taken up during in vitro growth, which raises the question for the fate and effect of the incorporated sorbitol.

ASJC Scopus Sachgebiete

  • Agrar- und Biowissenschaften (insg.)
  • Gartenbau

Ziele für nachhaltige Entwicklung

Zitieren

Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro: Is sorbitol taken up? / Bündig, C.; Blume, C.; Peterhänsel, C. et al.
in: Plant Cell, Tissue and Organ Culture, Jahrgang 127, Nr. 1, 10.2016, S. 195-206.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Download
@article{b467b6bfe87b4d948c7ac4be8011a7e9,
title = "Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro: Is sorbitol taken up?",
abstract = "Climate change and the increasing world population will lead to an increased water shortage. This gives rise to the need for plant cultivars which are drought tolerant. Solanum tuberosum L. is important not only as a nutritive rich food, but starch potatoes are of great value for the industry. Two starch genotypes of S. tuberosum L. divergently responding to osmotic stress were subjected to medium containing 0.2 M sorbitol in vitro. A targeted metabolomics approach was performed in which 42 metabolites were analysed 11 days after the transfer of the plants to the experimental medium. The sensitive genotype displayed stress responses comprising higher abundant metabolites such as phenylalanine, proline and sucrose and a decrease e.g. in GABA and fumaric acid. These can be used for protein build up, nitrogen storage and the protection through osmotic active compounds. In contrast, the tolerant genotype showed a higher abundance in compounds used as osmolytes (citric acid and proline), which might give rise to acclimatisation to the stress. Interestingly, in chromatograms of both genotypes a high sorbitol peak was detected, whereas control plants or plants treated with 4.8 % PEG 8000 did not accumulate this substance. Conclusively, sorbitol is taken up during in vitro growth, which raises the question for the fate and effect of the incorporated sorbitol.",
keywords = "Abiotic stress, In vitro, Proline, Solanum tuberosum, Sorbitol, Starch potato, Water deficiency",
author = "C. B{\"u}ndig and C. Blume and C. Peterh{\"a}nsel and T. Winkelmann",
year = "2016",
month = oct,
doi = "10.1007/s11240-016-1042-1",
language = "English",
volume = "127",
pages = "195--206",
journal = "Plant Cell, Tissue and Organ Culture",
issn = "0167-6857",
publisher = "Springer Netherlands",
number = "1",

}

Download

TY - JOUR

T1 - Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro

T2 - Is sorbitol taken up?

AU - Bündig, C.

AU - Blume, C.

AU - Peterhänsel, C.

AU - Winkelmann, T.

PY - 2016/10

Y1 - 2016/10

N2 - Climate change and the increasing world population will lead to an increased water shortage. This gives rise to the need for plant cultivars which are drought tolerant. Solanum tuberosum L. is important not only as a nutritive rich food, but starch potatoes are of great value for the industry. Two starch genotypes of S. tuberosum L. divergently responding to osmotic stress were subjected to medium containing 0.2 M sorbitol in vitro. A targeted metabolomics approach was performed in which 42 metabolites were analysed 11 days after the transfer of the plants to the experimental medium. The sensitive genotype displayed stress responses comprising higher abundant metabolites such as phenylalanine, proline and sucrose and a decrease e.g. in GABA and fumaric acid. These can be used for protein build up, nitrogen storage and the protection through osmotic active compounds. In contrast, the tolerant genotype showed a higher abundance in compounds used as osmolytes (citric acid and proline), which might give rise to acclimatisation to the stress. Interestingly, in chromatograms of both genotypes a high sorbitol peak was detected, whereas control plants or plants treated with 4.8 % PEG 8000 did not accumulate this substance. Conclusively, sorbitol is taken up during in vitro growth, which raises the question for the fate and effect of the incorporated sorbitol.

AB - Climate change and the increasing world population will lead to an increased water shortage. This gives rise to the need for plant cultivars which are drought tolerant. Solanum tuberosum L. is important not only as a nutritive rich food, but starch potatoes are of great value for the industry. Two starch genotypes of S. tuberosum L. divergently responding to osmotic stress were subjected to medium containing 0.2 M sorbitol in vitro. A targeted metabolomics approach was performed in which 42 metabolites were analysed 11 days after the transfer of the plants to the experimental medium. The sensitive genotype displayed stress responses comprising higher abundant metabolites such as phenylalanine, proline and sucrose and a decrease e.g. in GABA and fumaric acid. These can be used for protein build up, nitrogen storage and the protection through osmotic active compounds. In contrast, the tolerant genotype showed a higher abundance in compounds used as osmolytes (citric acid and proline), which might give rise to acclimatisation to the stress. Interestingly, in chromatograms of both genotypes a high sorbitol peak was detected, whereas control plants or plants treated with 4.8 % PEG 8000 did not accumulate this substance. Conclusively, sorbitol is taken up during in vitro growth, which raises the question for the fate and effect of the incorporated sorbitol.

KW - Abiotic stress

KW - In vitro

KW - Proline

KW - Solanum tuberosum

KW - Sorbitol

KW - Starch potato

KW - Water deficiency

UR - http://www.scopus.com/inward/record.url?scp=84978121137&partnerID=8YFLogxK

U2 - 10.1007/s11240-016-1042-1

DO - 10.1007/s11240-016-1042-1

M3 - Article

VL - 127

SP - 195

EP - 206

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 1

ER -

Von denselben Autoren