Biochemical properties of a novel cell wall protein associated with elongation growth in higher plants

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OriginalspracheEnglisch
Seiten (von - bis)1593-1601
Seitenumfang9
FachzeitschriftJournal of experimental botany
Jahrgang45
Ausgabenummer11
PublikationsstatusVeröffentlicht - 1 Nov. 1994

Abstract

A monoclonal antibody of IgM-type (TIM-11B2) was screened from a hybridoma library. The antibody recognizes a 40 kDa glycoprotein, p40, with high specificity. This protein was detected in all plant species examined so far and was found to be located both solubly and ionically-bound within the primary cell wall.The strongest immunobiochemical signals of p40 were found in tissues undergoing elongation growth, whereas in other tissues only a faint signal could be detected. Those included the non-elongating parts of different seedlings, such as the apical part of monocot primary leaves or the leaves of dicots grown in light. Inhibition of pea epicotyl growth by white light irradiation resulted in a strong decrease of the immunostain signal. On the other hand, induction of rapid coleoptile growth in rice seedlings induced by submergence resulted in a strong increase of the immunobiochemical signal of p40. Time-course studies on the expression of p40 during protoplast regeneration revealed that p40 is apparently not involved in cell wall formation. The hypothesis that p40 is characteristic for tissues with the ability for elongation growth is discussed.Comparison of biochemical data and location of p40 with proteins described up to now indicate that this glycoprotein has not been characterized before.

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Biochemical properties of a novel cell wall protein associated with elongation growth in higher plants. / Reinard, Thomas; Sprunck, Stefanie; Altherr, Sandra et al.
in: Journal of experimental botany, Jahrgang 45, Nr. 11, 01.11.1994, S. 1593-1601.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Reinard T, Sprunck S, Altherr S, Jacobsen HJ. Biochemical properties of a novel cell wall protein associated with elongation growth in higher plants. Journal of experimental botany. 1994 Nov 1;45(11):1593-1601. doi: 10.1093/jxb/45.11.1593
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abstract = "A monoclonal antibody of IgM-type (TIM-11B2) was screened from a hybridoma library. The antibody recognizes a 40 kDa glycoprotein, p40, with high specificity. This protein was detected in all plant species examined so far and was found to be located both solubly and ionically-bound within the primary cell wall.The strongest immunobiochemical signals of p40 were found in tissues undergoing elongation growth, whereas in other tissues only a faint signal could be detected. Those included the non-elongating parts of different seedlings, such as the apical part of monocot primary leaves or the leaves of dicots grown in light. Inhibition of pea epicotyl growth by white light irradiation resulted in a strong decrease of the immunostain signal. On the other hand, induction of rapid coleoptile growth in rice seedlings induced by submergence resulted in a strong increase of the immunobiochemical signal of p40. Time-course studies on the expression of p40 during protoplast regeneration revealed that p40 is apparently not involved in cell wall formation. The hypothesis that p40 is characteristic for tissues with the ability for elongation growth is discussed.Comparison of biochemical data and location of p40 with proteins described up to now indicate that this glycoprotein has not been characterized before.",
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AU - Jacobsen, Hans Jörg

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N2 - A monoclonal antibody of IgM-type (TIM-11B2) was screened from a hybridoma library. The antibody recognizes a 40 kDa glycoprotein, p40, with high specificity. This protein was detected in all plant species examined so far and was found to be located both solubly and ionically-bound within the primary cell wall.The strongest immunobiochemical signals of p40 were found in tissues undergoing elongation growth, whereas in other tissues only a faint signal could be detected. Those included the non-elongating parts of different seedlings, such as the apical part of monocot primary leaves or the leaves of dicots grown in light. Inhibition of pea epicotyl growth by white light irradiation resulted in a strong decrease of the immunostain signal. On the other hand, induction of rapid coleoptile growth in rice seedlings induced by submergence resulted in a strong increase of the immunobiochemical signal of p40. Time-course studies on the expression of p40 during protoplast regeneration revealed that p40 is apparently not involved in cell wall formation. The hypothesis that p40 is characteristic for tissues with the ability for elongation growth is discussed.Comparison of biochemical data and location of p40 with proteins described up to now indicate that this glycoprotein has not been characterized before.

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