Trichoderma reesei contains a biosynthetic gene cluster that encodes the antifungal agent ilicicolin h

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Original languageEnglish
Article number1034
JournalJournal of Fungi
Volume7
Issue number12
Publication statusPublished - 1 Dec 2021

Abstract

The trili biosynthetic gene cluster (BGC) from the well-studied organism Trichoderma reesei was studied by heterologous expression in the fungal host Aspergillus oryzae. Coexpression of triliA and triliB produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by triliC then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by triliD affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the T. reesei trili BGC encodes biosynthesis of this potent antifungal agent. Unexpected A. oryzae shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions.

Keywords

    Heterologous expression, Ilicicolin H, PKS-NRPS, Shunt metabolite, Trichoderma reesei

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Trichoderma reesei contains a biosynthetic gene cluster that encodes the antifungal agent ilicicolin h. / Shenouda, Mary L.; Ambilika, Maria; Cox, Russell J.
In: Journal of Fungi, Vol. 7, No. 12, 1034, 01.12.2021.

Research output: Contribution to journalArticleResearchpeer review

Shenouda ML, Ambilika M, Cox RJ. Trichoderma reesei contains a biosynthetic gene cluster that encodes the antifungal agent ilicicolin h. Journal of Fungi. 2021 Dec 1;7(12):1034. doi: 10.3390/jof7121034
Shenouda, Mary L. ; Ambilika, Maria ; Cox, Russell J. / Trichoderma reesei contains a biosynthetic gene cluster that encodes the antifungal agent ilicicolin h. In: Journal of Fungi. 2021 ; Vol. 7, No. 12.
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title = "Trichoderma reesei contains a biosynthetic gene cluster that encodes the antifungal agent ilicicolin h",
abstract = "The trili biosynthetic gene cluster (BGC) from the well-studied organism Trichoderma reesei was studied by heterologous expression in the fungal host Aspergillus oryzae. Coexpression of triliA and triliB produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by triliC then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by triliD affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the T. reesei trili BGC encodes biosynthesis of this potent antifungal agent. Unexpected A. oryzae shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions.",
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note = "Funding Information: Funding: M.L.S. thanks the German Academic Exchange Service (DAAD) and the Egyptian Ministry of Higher Education and Scientific Research (MHESR) for funding (GERLS programme, 2017, 57311832). This work was supported by DFG [CO 1328/2-1, INST 187/621-1, INST 187/686-1)]. The APC was funded by the Open Access Fund of the Leibniz Universit{\"a}t Hannover.",
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AU - Shenouda, Mary L.

AU - Ambilika, Maria

AU - Cox, Russell J.

N1 - Funding Information: Funding: M.L.S. thanks the German Academic Exchange Service (DAAD) and the Egyptian Ministry of Higher Education and Scientific Research (MHESR) for funding (GERLS programme, 2017, 57311832). This work was supported by DFG [CO 1328/2-1, INST 187/621-1, INST 187/686-1)]. The APC was funded by the Open Access Fund of the Leibniz Universität Hannover.

PY - 2021/12/1

Y1 - 2021/12/1

N2 - The trili biosynthetic gene cluster (BGC) from the well-studied organism Trichoderma reesei was studied by heterologous expression in the fungal host Aspergillus oryzae. Coexpression of triliA and triliB produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by triliC then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by triliD affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the T. reesei trili BGC encodes biosynthesis of this potent antifungal agent. Unexpected A. oryzae shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions.

AB - The trili biosynthetic gene cluster (BGC) from the well-studied organism Trichoderma reesei was studied by heterologous expression in the fungal host Aspergillus oryzae. Coexpression of triliA and triliB produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by triliC then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by triliD affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the T. reesei trili BGC encodes biosynthesis of this potent antifungal agent. Unexpected A. oryzae shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions.

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