Details
| Original language | English |
|---|---|
| Pages (from-to) | 1485-1495 |
| Number of pages | 11 |
| Journal | Plant cell reports |
| Volume | 27 |
| Issue number | 9 |
| Early online date | 3 Jul 2008 |
| Publication status | Published - Sept 2008 |
Abstract
Dwarf genotypes of the economically important flowering potted plant Kalanchoe blossfeldiana were developed by molecular breeding. Root inducing (Ri)-lines were regenerated by applying CPPU to the hairy roots, which were produced by inoculating leaf explants with a wild-type Agrobacterium rhizogenes strain ATCC15834. Amplification by polymerase chain reaction (PCR) and Southern blot analysis confirmed the presence of T-DNA in the Ri-lines. Six Ri-lines were characterised in a greenhouse trial revealing that several morphological traits changed with respect to ornamental value such as plant height, number of lateral shoots, leaf size, leaf number, flower size and number of flowers. The Ri-lines differed in their degree of Ri-phenotype, and the internodes of the Ri-lines were clearly shorter, giving a compact growth habit compared to control plants. Time to anthesis was the same in Ri-line 331 as in control plants and delayed by only 3 days in Ri-line 306 as compared to control plants. A compact plant without delayed flowering can be assumed to be valuable for further breeding.
Keywords
- Hairy roots, Ornamental plants, Ri-plasmid
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant cell reports, Vol. 27, No. 9, 09.2008, p. 1485-1495.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transformation of Kalanchoe blossfeldiana with rol-genes is useful in molecular breeding towards compact growth
AU - Christensen, Brian
AU - Sriskandarajah, Sridevy
AU - Serek, Margrethe
AU - Müller, Renate
N1 - Funding Information: Acknowledgments The study was funded by a PhD grant from the University of Copenhagen, Faculty of Life Sciences and the authors would like to thank Knud Jepsen A/S, Hinnerup, Denmark, for providing plant material for the study.
PY - 2008/9
Y1 - 2008/9
N2 - Dwarf genotypes of the economically important flowering potted plant Kalanchoe blossfeldiana were developed by molecular breeding. Root inducing (Ri)-lines were regenerated by applying CPPU to the hairy roots, which were produced by inoculating leaf explants with a wild-type Agrobacterium rhizogenes strain ATCC15834. Amplification by polymerase chain reaction (PCR) and Southern blot analysis confirmed the presence of T-DNA in the Ri-lines. Six Ri-lines were characterised in a greenhouse trial revealing that several morphological traits changed with respect to ornamental value such as plant height, number of lateral shoots, leaf size, leaf number, flower size and number of flowers. The Ri-lines differed in their degree of Ri-phenotype, and the internodes of the Ri-lines were clearly shorter, giving a compact growth habit compared to control plants. Time to anthesis was the same in Ri-line 331 as in control plants and delayed by only 3 days in Ri-line 306 as compared to control plants. A compact plant without delayed flowering can be assumed to be valuable for further breeding.
AB - Dwarf genotypes of the economically important flowering potted plant Kalanchoe blossfeldiana were developed by molecular breeding. Root inducing (Ri)-lines were regenerated by applying CPPU to the hairy roots, which were produced by inoculating leaf explants with a wild-type Agrobacterium rhizogenes strain ATCC15834. Amplification by polymerase chain reaction (PCR) and Southern blot analysis confirmed the presence of T-DNA in the Ri-lines. Six Ri-lines were characterised in a greenhouse trial revealing that several morphological traits changed with respect to ornamental value such as plant height, number of lateral shoots, leaf size, leaf number, flower size and number of flowers. The Ri-lines differed in their degree of Ri-phenotype, and the internodes of the Ri-lines were clearly shorter, giving a compact growth habit compared to control plants. Time to anthesis was the same in Ri-line 331 as in control plants and delayed by only 3 days in Ri-line 306 as compared to control plants. A compact plant without delayed flowering can be assumed to be valuable for further breeding.
KW - Hairy roots
KW - Ornamental plants
KW - Ri-plasmid
UR - http://www.scopus.com/inward/record.url?scp=49349108562&partnerID=8YFLogxK
U2 - 10.1007/s00299-008-0575-0
DO - 10.1007/s00299-008-0575-0
M3 - Article
C2 - 18597094
AN - SCOPUS:49349108562
VL - 27
SP - 1485
EP - 1495
JO - Plant cell reports
JF - Plant cell reports
SN - 0721-7714
IS - 9
ER -