Details
| Original language | English |
|---|---|
| Pages (from-to) | 303-318 |
| Number of pages | 16 |
| Journal | Plant molecular biology |
| Volume | 94 |
| Issue number | 3 |
| Early online date | 19 Apr 2017 |
| Publication status | Published - 1 Jun 2017 |
Abstract
Key message: Gene expression studies in roots of apple replant disease affected plants suggested defense reactions towards biotic stress to occur which did not lead to adequate responses to the biotic stressors. Abstract: Apple replant disease (ARD) leads to growth inhibition and fruit yield reduction in replanted populations and results in economic losses for tree nurseries and fruit producers. The etiology is not well understood on a molecular level and causal agents show a great diversity indicating that no definitive cause, which applies to the majority of cases, has been found out yet. Hence, it is pivotal to gain a better understanding of the molecular and physiological reactions of the plant when affected by ARD and later to overcome the disease, for example by developing tolerant rootstocks. For the first time, gene expression was investigated in roots of ARD affected plants employing massive analysis of cDNA ends (MACE) and RT-qPCR. In reaction to ARD, genes in secondary metabolite production as well as plant defense, regulatory and signaling genes were upregulated whereas for several genes involved in primary metabolism lower expression was detected. For internal verification of MACE data, candidate genes were tested via RT-qPCR and a strong positive correlation between both datasets was observed. Comparison of apple ‘M26’ roots cultivated in ARD soil or γ-irradiated ARD soil suggests that typical defense reactions towards biotic stress take place in ARD affected plants but they did not allow responding to the biotic stressors attack adequately, leading to the observed growth depressions in ARD variants.
Keywords
- Biotic stress response, Gene expression, Growth depression, MACE, Phytoalexins, Plant defense, Quantitative real-time PCR
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant molecular biology, Vol. 94, No. 3, 01.06.2017, p. 303-318.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transcriptomic analysis of molecular responses in Malus domestica ‘M26’ roots affected by apple replant disease
AU - Weiß, Stefan
AU - Bartsch, Melanie
AU - Winkelmann, Traud
N1 - Publisher Copyright: © 2017, Springer Science+Business Media Dordrecht.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Key message: Gene expression studies in roots of apple replant disease affected plants suggested defense reactions towards biotic stress to occur which did not lead to adequate responses to the biotic stressors. Abstract: Apple replant disease (ARD) leads to growth inhibition and fruit yield reduction in replanted populations and results in economic losses for tree nurseries and fruit producers. The etiology is not well understood on a molecular level and causal agents show a great diversity indicating that no definitive cause, which applies to the majority of cases, has been found out yet. Hence, it is pivotal to gain a better understanding of the molecular and physiological reactions of the plant when affected by ARD and later to overcome the disease, for example by developing tolerant rootstocks. For the first time, gene expression was investigated in roots of ARD affected plants employing massive analysis of cDNA ends (MACE) and RT-qPCR. In reaction to ARD, genes in secondary metabolite production as well as plant defense, regulatory and signaling genes were upregulated whereas for several genes involved in primary metabolism lower expression was detected. For internal verification of MACE data, candidate genes were tested via RT-qPCR and a strong positive correlation between both datasets was observed. Comparison of apple ‘M26’ roots cultivated in ARD soil or γ-irradiated ARD soil suggests that typical defense reactions towards biotic stress take place in ARD affected plants but they did not allow responding to the biotic stressors attack adequately, leading to the observed growth depressions in ARD variants.
AB - Key message: Gene expression studies in roots of apple replant disease affected plants suggested defense reactions towards biotic stress to occur which did not lead to adequate responses to the biotic stressors. Abstract: Apple replant disease (ARD) leads to growth inhibition and fruit yield reduction in replanted populations and results in economic losses for tree nurseries and fruit producers. The etiology is not well understood on a molecular level and causal agents show a great diversity indicating that no definitive cause, which applies to the majority of cases, has been found out yet. Hence, it is pivotal to gain a better understanding of the molecular and physiological reactions of the plant when affected by ARD and later to overcome the disease, for example by developing tolerant rootstocks. For the first time, gene expression was investigated in roots of ARD affected plants employing massive analysis of cDNA ends (MACE) and RT-qPCR. In reaction to ARD, genes in secondary metabolite production as well as plant defense, regulatory and signaling genes were upregulated whereas for several genes involved in primary metabolism lower expression was detected. For internal verification of MACE data, candidate genes were tested via RT-qPCR and a strong positive correlation between both datasets was observed. Comparison of apple ‘M26’ roots cultivated in ARD soil or γ-irradiated ARD soil suggests that typical defense reactions towards biotic stress take place in ARD affected plants but they did not allow responding to the biotic stressors attack adequately, leading to the observed growth depressions in ARD variants.
KW - Biotic stress response
KW - Gene expression
KW - Growth depression
KW - MACE
KW - Phytoalexins
KW - Plant defense
KW - Quantitative real-time PCR
UR - http://www.scopus.com/inward/record.url?scp=85018471521&partnerID=8YFLogxK
U2 - 10.1007/s11103-017-0608-6
DO - 10.1007/s11103-017-0608-6
M3 - Article
C2 - 28424966
AN - SCOPUS:85018471521
VL - 94
SP - 303
EP - 318
JO - Plant molecular biology
JF - Plant molecular biology
SN - 0167-4412
IS - 3
ER -