The 2-oxoglutarate/malate translocator mediates amino acid and storage protein biosynthesis in pea embryos

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Erik Riebeseel
  • Rainer E. Häusler
  • Ruslana Radchuk
  • Tobias Meitzel
  • Mohammad Reza Hajirezaei
  • R. J.Neil Emery
  • Helge Küster
  • Adriano Nunes-Nesi
  • Alisdair R. Fernie
  • Winfriede Weschke
  • Hans Weber

External Research Organisations

  • Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
  • University of Cologne
  • Trent University
  • Max Planck Institute of Molecular Plant Physiology (MPI-MP)
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Details

Original languageEnglish
Pages (from-to)350-363
Number of pages14
JournalPlant Journal
Volume61
Issue number2
Publication statusPublished - 1 Jan 2010

Abstract

Heterotrophic plastids of seeds perform many biosynthetic reactions. Understanding their function in crop plants is crucial for seed production. Physiological functions depend on the uptake of precursors by a range of different metabolite translocators. The 2-oxoglutarate/malate translocator gene (PsOMT), which is highly expressed during pea (Pisum sativum) embryo maturation, has an important role during seed storage. PsOMT functions have been studied by antisense repression in maturing pea embryos, and were found to reduce mRNA levels and transport rates of 2-oxoglutarate and malate by 50-70%. Combined metabolite and transcript profiling revealed that OMT repression affects the conversion of carbohydrates from sucrose into amino acids and proteins, decreases seed weight and delays maturation. OMT-repressed pea embryos have increased levels of organic acids, ammonia, and higher ratios of Asn : Asp and Gln : Glu. Decreased levels of most other amino acids indicate the reduced usage of organic acids and ammonia for amino acid biosynthesis in plastids, possibly caused by substrate limitation of the plastidial glutamine synthetase/glutamine- 2-oxoglutarate aminotransferase cycle. Expression of storage proteins is delayed, and mature seeds have reduced protein content. Downregulated gene expression of starch biosynthesis and plastidial glucose-6-phosphate transport in asOMT embryos reveals that decreased 2-oxoglutarate/malate transport capacity affects other pathways of central carbon metabolism. Gene expression analysis related to plastid physiology revealed that OMT repression delays differentiation of storage plastids, thereby maintaining gene expression associated with green chloroplasts. We conclude that OMT is important for protein-storing crop seeds, and is necessary for amino acid biosynthesis in pea seeds. In addition, carbon supply as mediated by OMT controls plastid differentiation during seed maturation.

Keywords

    2-oxoglutarate/malate translocator, Amino acid metabolism, Legume seed maturation, Metabolic regulation, Plastids, Storage proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics
  • Agricultural and Biological Sciences(all)
  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

The 2-oxoglutarate/malate translocator mediates amino acid and storage protein biosynthesis in pea embryos. / Riebeseel, Erik; Häusler, Rainer E.; Radchuk, Ruslana et al.
In: Plant Journal, Vol. 61, No. 2, 01.01.2010, p. 350-363.

Research output: Contribution to journalArticleResearchpeer review

Riebeseel, E, Häusler, RE, Radchuk, R, Meitzel, T, Hajirezaei, MR, Emery, RJN, Küster, H, Nunes-Nesi, A, Fernie, AR, Weschke, W & Weber, H 2010, 'The 2-oxoglutarate/malate translocator mediates amino acid and storage protein biosynthesis in pea embryos', Plant Journal, vol. 61, no. 2, pp. 350-363. https://doi.org/10.1111/j.1365-313X.2009.04058.x
Riebeseel, E., Häusler, R. E., Radchuk, R., Meitzel, T., Hajirezaei, M. R., Emery, R. J. N., Küster, H., Nunes-Nesi, A., Fernie, A. R., Weschke, W., & Weber, H. (2010). The 2-oxoglutarate/malate translocator mediates amino acid and storage protein biosynthesis in pea embryos. Plant Journal, 61(2), 350-363. https://doi.org/10.1111/j.1365-313X.2009.04058.x
Riebeseel E, Häusler RE, Radchuk R, Meitzel T, Hajirezaei MR, Emery RJN et al. The 2-oxoglutarate/malate translocator mediates amino acid and storage protein biosynthesis in pea embryos. Plant Journal. 2010 Jan 1;61(2):350-363. doi: 10.1111/j.1365-313X.2009.04058.x
Riebeseel, Erik ; Häusler, Rainer E. ; Radchuk, Ruslana et al. / The 2-oxoglutarate/malate translocator mediates amino acid and storage protein biosynthesis in pea embryos. In: Plant Journal. 2010 ; Vol. 61, No. 2. pp. 350-363.
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AU - Meitzel, Tobias

AU - Hajirezaei, Mohammad Reza

AU - Emery, R. J.Neil

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