Details
| Original language | English |
|---|---|
| Pages (from-to) | 4009-4013 |
| Number of pages | 5 |
| Journal | CHEMCATCHEM |
| Volume | 12 |
| Issue number | 16 |
| Early online date | 24 Apr 2020 |
| Publication status | Published - 21 Aug 2020 |
| Externally published | Yes |
Abstract
The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase from Agrocybe aegerita (AaeUPO) can hydroxylate the neat model substrate cyclohexane. H2O2 was photocatalytically generated in situ by nitrogen-doped carbon nanodots (N−CNDs) and UV LED illumination. AaeUPO entrapment in alginate beads increased enzyme stability and facilitated the reaction in neat cyclohexane. N−CNDs absorption in beads containing AaeUPO created a 2-in-1 heterogeneous photobiocatalyst that was active for up to seven days under reaction conditions and produced cyclohexanol, 2.5 mM. To increase productivity, the bead size and the photocatalyst-to-enzyme ratio have been identified as promising targets for optimisation.
Keywords
- carbon nanodots, hydroxylation, non-conventional media, organic media, Photobiocatalysis
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemistry(all)
- Organic Chemistry
- Chemistry(all)
- Inorganic Chemistry
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In: CHEMCATCHEM, Vol. 12, No. 16, 21.08.2020, p. 4009-4013.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Solvent-Free Photobiocatalytic Hydroxylation of Cyclohexane
AU - Hobisch, Markus
AU - van Schie, Morten Martinus Cornelis Harald
AU - Kim, Jinhyun
AU - Røjkjær Andersen, Kasper
AU - Alcalde, Miguel
AU - Kourist, Robert
AU - Park, Chan Beum
AU - Hollmann, Frank
AU - Kara, Selin
N1 - Funding Information: Kristian Graf and Henrik Hartvig Kolmos, Department of Molecular Biology of Genetics, are gratefully acknowledged for the construction of screening scale photoreactor. Kim Møller Johansen and Bekir Engin Eser, Department of Engineering, are acknowledged for their technical support in GC analytics. Klaus Koren, Department of Bioscience, is gratefully acknowledged for spectroscopic measurements with Gigahertz‐Optik MSC15. Peter Leon Hagedoorn, Department of Biotechnology, TU Delft, is acknowledged for his technical support in EPR analytics. This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska‐Curie grant agreement No 764920.
PY - 2020/8/21
Y1 - 2020/8/21
N2 - The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase from Agrocybe aegerita (AaeUPO) can hydroxylate the neat model substrate cyclohexane. H2O2 was photocatalytically generated in situ by nitrogen-doped carbon nanodots (N−CNDs) and UV LED illumination. AaeUPO entrapment in alginate beads increased enzyme stability and facilitated the reaction in neat cyclohexane. N−CNDs absorption in beads containing AaeUPO created a 2-in-1 heterogeneous photobiocatalyst that was active for up to seven days under reaction conditions and produced cyclohexanol, 2.5 mM. To increase productivity, the bead size and the photocatalyst-to-enzyme ratio have been identified as promising targets for optimisation.
AB - The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase from Agrocybe aegerita (AaeUPO) can hydroxylate the neat model substrate cyclohexane. H2O2 was photocatalytically generated in situ by nitrogen-doped carbon nanodots (N−CNDs) and UV LED illumination. AaeUPO entrapment in alginate beads increased enzyme stability and facilitated the reaction in neat cyclohexane. N−CNDs absorption in beads containing AaeUPO created a 2-in-1 heterogeneous photobiocatalyst that was active for up to seven days under reaction conditions and produced cyclohexanol, 2.5 mM. To increase productivity, the bead size and the photocatalyst-to-enzyme ratio have been identified as promising targets for optimisation.
KW - carbon nanodots
KW - hydroxylation
KW - non-conventional media
KW - organic media
KW - Photobiocatalysis
UR - http://www.scopus.com/inward/record.url?scp=85086247340&partnerID=8YFLogxK
U2 - 10.1002/cctc.202000512
DO - 10.1002/cctc.202000512
M3 - Article
AN - SCOPUS:85086247340
VL - 12
SP - 4009
EP - 4013
JO - CHEMCATCHEM
JF - CHEMCATCHEM
SN - 1867-3880
IS - 16
ER -