Details
| Original language | English |
|---|---|
| Pages (from-to) | 1596-1602 |
| Number of pages | 7 |
| Journal | Journal of separation science |
| Volume | 35 |
| Issue number | 13 |
| Publication status | Published - 4 Jul 2012 |
Abstract
Potato fruit juice as a by-product of the starch industry contains proteins with interesting functionalities such as protease inhibitors or patatin with its high nutritional value. Due to their functional properties, these proteins are principally of industrial interest. A drawback for the application of these potato proteins is the separation and isolation under maintenance of the biological activity. So far, there are no methods in literature, which are satisfying concerning the costs or the separation performance. In this study, we show a chromatographic approach using natural clay minerals as cation exchangers to separate two protein fractions in potato fruit juice. Additionally, the content of glycoalkaloids naturally occurring in potatoes is significantly reduced in a single step together with the separation of the patatins and the protease inhibitors.
Keywords
- Cation exchanger, Clay minerals, Glycoalkaloid reduction, Potato fruit juice, Protein separation
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Chemical Engineering(all)
- Filtration and Separation
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In: Journal of separation science, Vol. 35, No. 13, 04.07.2012, p. 1596-1602.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Separation of patatins and protease inhibitors from potato fruit juice with clay minerals as cation exchangers
AU - Ralla, Kathrin
AU - Sohling, Ulrich
AU - Suck, Kirstin
AU - Kasper, Cornelia
AU - Ruf, Friedrich
AU - Scheper, Thomas
PY - 2012/7/4
Y1 - 2012/7/4
N2 - Potato fruit juice as a by-product of the starch industry contains proteins with interesting functionalities such as protease inhibitors or patatin with its high nutritional value. Due to their functional properties, these proteins are principally of industrial interest. A drawback for the application of these potato proteins is the separation and isolation under maintenance of the biological activity. So far, there are no methods in literature, which are satisfying concerning the costs or the separation performance. In this study, we show a chromatographic approach using natural clay minerals as cation exchangers to separate two protein fractions in potato fruit juice. Additionally, the content of glycoalkaloids naturally occurring in potatoes is significantly reduced in a single step together with the separation of the patatins and the protease inhibitors.
AB - Potato fruit juice as a by-product of the starch industry contains proteins with interesting functionalities such as protease inhibitors or patatin with its high nutritional value. Due to their functional properties, these proteins are principally of industrial interest. A drawback for the application of these potato proteins is the separation and isolation under maintenance of the biological activity. So far, there are no methods in literature, which are satisfying concerning the costs or the separation performance. In this study, we show a chromatographic approach using natural clay minerals as cation exchangers to separate two protein fractions in potato fruit juice. Additionally, the content of glycoalkaloids naturally occurring in potatoes is significantly reduced in a single step together with the separation of the patatins and the protease inhibitors.
KW - Cation exchanger
KW - Clay minerals
KW - Glycoalkaloid reduction
KW - Potato fruit juice
KW - Protein separation
UR - http://www.scopus.com/inward/record.url?scp=84863668186&partnerID=8YFLogxK
U2 - 10.1002/jssc.201200030
DO - 10.1002/jssc.201200030
M3 - Article
C2 - 22761137
AN - SCOPUS:84863668186
VL - 35
SP - 1596
EP - 1602
JO - Journal of separation science
JF - Journal of separation science
SN - 1615-9306
IS - 13
ER -