Details
| Original language | English |
|---|---|
| Article number | e70529 |
| Journal | Physiologia plantarum |
| Volume | 177 |
| Issue number | 5 |
| Publication status | Published - 26 Sept 2025 |
Abstract
Guard cells are highly specialised leaf cells which regulate leaf transpiration and carbon fixation. As such, they are instrumental in balancing water use efficiency and photosynthetic activity. This unique function of guard cells requires them to quickly accumulate solutes for ATP-dependent stomatal opening, but how this affects mitochondrial energy metabolism remains elusive. Using cell-type-specific affinity purification of Arabidopsis thaliana guard cell and mesophyll cell mitochondria, we aim at unravelling the enzymatic configuration of guard cell mitochondria in order to provide a first glance at the metabolic properties of these specialised organelles. When compared to their mesophyll cell counterparts, the use of alternative substrates for NADH production, a shift away from non-proton pumping respiratory enzymes, and a lower NADH re-oxidation rate suggest a specialised mode of ATP production in guard cell mitochondria. In addition, a lowered abundance of the mitochondrial import machinery also indirectly implies lower protein turnover rates in these organelles.
Keywords
- amino acid catabolism, energy metabolism, guard cells, mitochondria, proteomics
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Physiologia plantarum, Vol. 177, No. 5, e70529, 26.09.2025.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Proteomic Analysis Infers Optimized ATP-Production in Guard Cell Mitochondria
AU - Ditz, Noah
AU - Niehaus, Markus
AU - Medina Escobar, Nieves
AU - Herde, Marco
AU - Eubel, Holger
N1 - Publisher Copyright: © 2025 The Author(s). Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.
PY - 2025/9/26
Y1 - 2025/9/26
N2 - Guard cells are highly specialised leaf cells which regulate leaf transpiration and carbon fixation. As such, they are instrumental in balancing water use efficiency and photosynthetic activity. This unique function of guard cells requires them to quickly accumulate solutes for ATP-dependent stomatal opening, but how this affects mitochondrial energy metabolism remains elusive. Using cell-type-specific affinity purification of Arabidopsis thaliana guard cell and mesophyll cell mitochondria, we aim at unravelling the enzymatic configuration of guard cell mitochondria in order to provide a first glance at the metabolic properties of these specialised organelles. When compared to their mesophyll cell counterparts, the use of alternative substrates for NADH production, a shift away from non-proton pumping respiratory enzymes, and a lower NADH re-oxidation rate suggest a specialised mode of ATP production in guard cell mitochondria. In addition, a lowered abundance of the mitochondrial import machinery also indirectly implies lower protein turnover rates in these organelles.
AB - Guard cells are highly specialised leaf cells which regulate leaf transpiration and carbon fixation. As such, they are instrumental in balancing water use efficiency and photosynthetic activity. This unique function of guard cells requires them to quickly accumulate solutes for ATP-dependent stomatal opening, but how this affects mitochondrial energy metabolism remains elusive. Using cell-type-specific affinity purification of Arabidopsis thaliana guard cell and mesophyll cell mitochondria, we aim at unravelling the enzymatic configuration of guard cell mitochondria in order to provide a first glance at the metabolic properties of these specialised organelles. When compared to their mesophyll cell counterparts, the use of alternative substrates for NADH production, a shift away from non-proton pumping respiratory enzymes, and a lower NADH re-oxidation rate suggest a specialised mode of ATP production in guard cell mitochondria. In addition, a lowered abundance of the mitochondrial import machinery also indirectly implies lower protein turnover rates in these organelles.
KW - amino acid catabolism
KW - energy metabolism
KW - guard cells
KW - mitochondria
KW - proteomics
UR - http://www.scopus.com/inward/record.url?scp=105017130012&partnerID=8YFLogxK
U2 - 10.1111/ppl.70529
DO - 10.1111/ppl.70529
M3 - Article
C2 - 40999960
AN - SCOPUS:105017130012
VL - 177
JO - Physiologia plantarum
JF - Physiologia plantarum
SN - 0031-9317
IS - 5
M1 - e70529
ER -