Details
| Original language | English |
|---|---|
| Pages (from-to) | 825-833 |
| Number of pages | 9 |
| Journal | Journal of Biophotonics |
| Volume | 7 |
| Issue number | 10 |
| Publication status | Published - 6 Jun 2013 |
| Externally published | Yes |
Abstract
Several cell transfection techniques have been developed in the last decades for specific applications and for various types of molecules. In this context, laser based approaches are of great interest due to their minimal invasiveness and spatial selectivity. In particular, laser induced plasmon based delivery of exogenous molecules into cells can have great impact on future applications. This approach allows high-throughput laser transfection by excitation of plasmon resonances at gold nanoparticles non-specifically attached to the cell membrane. In this study, we demonstrate specific gene-knockdown by transfection of Morpholino oligos using this technique with optimized particle size. Furthermore, we evaluated the cytotoxicity of plasmonic laser treatment by various assays, including LDH activity and ROS formation. In summary, this study gives important insights into this new approach and clearly demonstrates its relevance for possible biological applications.
Keywords
- Gold nanoparticle, Knockdown, Laser transfection, Morpholino, Plasmon
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Materials Science(all)
- General Materials Science
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Engineering(all)
- General Engineering
- Physics and Astronomy(all)
- General Physics and Astronomy
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In: Journal of Biophotonics, Vol. 7, No. 10, 06.06.2013, p. 825-833.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Plasmonic laser treatment for Morpholino oligomer delivery in antisense applications
AU - Kalies, Stefan
AU - Heinemann, Dag
AU - Schomaker, Markus
AU - Murua Escobar, Hugo
AU - Heisterkamp, Alexander
AU - Ripken, Tammo
AU - Meyer, Heiko
PY - 2013/6/6
Y1 - 2013/6/6
N2 - Several cell transfection techniques have been developed in the last decades for specific applications and for various types of molecules. In this context, laser based approaches are of great interest due to their minimal invasiveness and spatial selectivity. In particular, laser induced plasmon based delivery of exogenous molecules into cells can have great impact on future applications. This approach allows high-throughput laser transfection by excitation of plasmon resonances at gold nanoparticles non-specifically attached to the cell membrane. In this study, we demonstrate specific gene-knockdown by transfection of Morpholino oligos using this technique with optimized particle size. Furthermore, we evaluated the cytotoxicity of plasmonic laser treatment by various assays, including LDH activity and ROS formation. In summary, this study gives important insights into this new approach and clearly demonstrates its relevance for possible biological applications.
AB - Several cell transfection techniques have been developed in the last decades for specific applications and for various types of molecules. In this context, laser based approaches are of great interest due to their minimal invasiveness and spatial selectivity. In particular, laser induced plasmon based delivery of exogenous molecules into cells can have great impact on future applications. This approach allows high-throughput laser transfection by excitation of plasmon resonances at gold nanoparticles non-specifically attached to the cell membrane. In this study, we demonstrate specific gene-knockdown by transfection of Morpholino oligos using this technique with optimized particle size. Furthermore, we evaluated the cytotoxicity of plasmonic laser treatment by various assays, including LDH activity and ROS formation. In summary, this study gives important insights into this new approach and clearly demonstrates its relevance for possible biological applications.
KW - Gold nanoparticle
KW - Knockdown
KW - Laser transfection
KW - Morpholino
KW - Plasmon
UR - http://www.scopus.com/inward/record.url?scp=84908567240&partnerID=8YFLogxK
U2 - 10.1002/jbio.201300056
DO - 10.1002/jbio.201300056
M3 - Article
C2 - 23740874
AN - SCOPUS:84908567240
VL - 7
SP - 825
EP - 833
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 10
ER -