Details
| Original language | English |
|---|---|
| Pages (from-to) | 361-372 |
| Number of pages | 12 |
| Journal | Engineering in Life Sciences |
| Volume | 22 |
| Issue number | 3-4 |
| Publication status | Published - 28 Mar 2022 |
Abstract
The utilization of mesenchymal stem/stromal cells raises new hopes in treatment of diseases and pathological conditions, while at the same time bringing immense challenges for researchers, manufacturers and physicians. It is essential to consider all steps along the in vitro fabrication of cell-based products in order to reach efficient and reproducible treatment outcomes. Here, the optimal protocols for isolation, cultivation and differentiation of mesenchymal stem cells are required. In this review we discuss these aspects and their influence on the final cell-based product quality. We demonstrate that physiological in vitro cell cultivation conditions play a crucial role in therapeutic functionalities of cultivated cells. We show that three-dimensional cell culture, dynamic culture conditions and physiologically relevant in vitro oxygen concentrations during isolation and expansion make a decisive contribution towards the improvement of cell-based products in regenerative medicine.
Keywords
- 3D cell culture, expansion, hypoxia, isolation, mesenchymal stem/stromal cells
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Environmental Science(all)
- Environmental Engineering
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In: Engineering in Life Sciences, Vol. 22, No. 3-4, 28.03.2022, p. 361-372.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Physiologic isolation and expansion of human mesenchymal stem/stromal cells for manufacturing of cell-based therapy products
AU - Egger, Dominik
AU - Lavrentieva, Antonina
AU - Kugelmeier, Patrick
AU - Kasper, Cornelia
N1 - Funding Information: This work is dedicated to Prof. Thomas Scheper ‐ our ever inspiring teacher and mentor, who guided generations of scientists and shaped the field of biotechnology. AL research is supported by German Research Foundation (DFG Projects 398007461 488 “3D Dual‐Gradient Systems for Functional Cell Screening” and “Biomolecular Sensor Platform for Elucidating Hypoxic Signatures in 2D and 3D in vitro culture Systems”). DE and CK research is supported by the Austrian Research Promotion Agency (FFG), project number 859279 and the DECHEMA Max‐Buchner Fellowship 3710. The authors thank Ilias Nikolits from University of Natural Resources and Life Sciences for preparation of the figures.
PY - 2022/3/28
Y1 - 2022/3/28
N2 - The utilization of mesenchymal stem/stromal cells raises new hopes in treatment of diseases and pathological conditions, while at the same time bringing immense challenges for researchers, manufacturers and physicians. It is essential to consider all steps along the in vitro fabrication of cell-based products in order to reach efficient and reproducible treatment outcomes. Here, the optimal protocols for isolation, cultivation and differentiation of mesenchymal stem cells are required. In this review we discuss these aspects and their influence on the final cell-based product quality. We demonstrate that physiological in vitro cell cultivation conditions play a crucial role in therapeutic functionalities of cultivated cells. We show that three-dimensional cell culture, dynamic culture conditions and physiologically relevant in vitro oxygen concentrations during isolation and expansion make a decisive contribution towards the improvement of cell-based products in regenerative medicine.
AB - The utilization of mesenchymal stem/stromal cells raises new hopes in treatment of diseases and pathological conditions, while at the same time bringing immense challenges for researchers, manufacturers and physicians. It is essential to consider all steps along the in vitro fabrication of cell-based products in order to reach efficient and reproducible treatment outcomes. Here, the optimal protocols for isolation, cultivation and differentiation of mesenchymal stem cells are required. In this review we discuss these aspects and their influence on the final cell-based product quality. We demonstrate that physiological in vitro cell cultivation conditions play a crucial role in therapeutic functionalities of cultivated cells. We show that three-dimensional cell culture, dynamic culture conditions and physiologically relevant in vitro oxygen concentrations during isolation and expansion make a decisive contribution towards the improvement of cell-based products in regenerative medicine.
KW - 3D cell culture
KW - expansion
KW - hypoxia
KW - isolation
KW - mesenchymal stem/stromal cells
UR - http://www.scopus.com/inward/record.url?scp=85118230379&partnerID=8YFLogxK
U2 - 10.1002/elsc.202100097
DO - 10.1002/elsc.202100097
M3 - Review article
AN - SCOPUS:85118230379
VL - 22
SP - 361
EP - 372
JO - Engineering in Life Sciences
JF - Engineering in Life Sciences
SN - 1618-0240
IS - 3-4
ER -