Details
| Original language | English |
|---|---|
| Pages (from-to) | 5627-5635 |
| Number of pages | 9 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 417 |
| Issue number | 25 |
| Early online date | 2 Oct 2025 |
| Publication status | Published - Oct 2025 |
Abstract
Monitoring key parameters during the cultivation of microorganisms—including biomass concentration—is often a crucial prerequisite for attaining reproducible results in the field of biotechnology. In order to overcome the drawbacks associated with conventional methods for assessing biomass concentration (such as cell dry weight or optical density measurements), several devices to facilitate continuous online monitoring have been developed. Yet despite the success of innovative solutions (such as acceleration sensors) in addressing the challenging dynamical behavior of liquids within shaken systems, the performance of these sensors can still be affected by reflections at the liquid–air interface or at the top of the cultivation vessels. In our previous work, a 3D-printed cultivation vessel equipped with modified optical waveguide paths was utilized in an effort to overcome this challenge, allowing for the measurement of scattered light in a lateral direction. In this work, we developed an optical adapter that allows for the rapid assessment of the optimal measurement angle of excitation and detection within these 3D-printed cultivation vessels. The findings of the present study indicate that an angle of 110° yields higher signal intensities and enhanced sensitivity in comparison to larger angles, and this finding was additionally confirmed for a set of biotechnologically relevant microorganisms. While these results suggest that the angle of 110° should be integrated into the cultivation vessels in the future, the optical adapter also holds the potential to further investigate vessels with different sizes or geometries.
Keywords
- 3D printing, Light scattering, Online monitoring, Optical waveguide
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
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In: Analytical and Bioanalytical Chemistry, Vol. 417, No. 25, 10.2025, p. 5627-5635.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Optimization of the angle for scattered light measurements in 3D-printed cultivation vessels
AU - Debener, Nicolas
AU - Kuhnke, Louis Maximilian
AU - Beutel, Sascha
AU - Bahnemann, Janina
N1 - Publisher Copyright: © The Author(s) 2025.
PY - 2025/10
Y1 - 2025/10
N2 - Monitoring key parameters during the cultivation of microorganisms—including biomass concentration—is often a crucial prerequisite for attaining reproducible results in the field of biotechnology. In order to overcome the drawbacks associated with conventional methods for assessing biomass concentration (such as cell dry weight or optical density measurements), several devices to facilitate continuous online monitoring have been developed. Yet despite the success of innovative solutions (such as acceleration sensors) in addressing the challenging dynamical behavior of liquids within shaken systems, the performance of these sensors can still be affected by reflections at the liquid–air interface or at the top of the cultivation vessels. In our previous work, a 3D-printed cultivation vessel equipped with modified optical waveguide paths was utilized in an effort to overcome this challenge, allowing for the measurement of scattered light in a lateral direction. In this work, we developed an optical adapter that allows for the rapid assessment of the optimal measurement angle of excitation and detection within these 3D-printed cultivation vessels. The findings of the present study indicate that an angle of 110° yields higher signal intensities and enhanced sensitivity in comparison to larger angles, and this finding was additionally confirmed for a set of biotechnologically relevant microorganisms. While these results suggest that the angle of 110° should be integrated into the cultivation vessels in the future, the optical adapter also holds the potential to further investigate vessels with different sizes or geometries.
AB - Monitoring key parameters during the cultivation of microorganisms—including biomass concentration—is often a crucial prerequisite for attaining reproducible results in the field of biotechnology. In order to overcome the drawbacks associated with conventional methods for assessing biomass concentration (such as cell dry weight or optical density measurements), several devices to facilitate continuous online monitoring have been developed. Yet despite the success of innovative solutions (such as acceleration sensors) in addressing the challenging dynamical behavior of liquids within shaken systems, the performance of these sensors can still be affected by reflections at the liquid–air interface or at the top of the cultivation vessels. In our previous work, a 3D-printed cultivation vessel equipped with modified optical waveguide paths was utilized in an effort to overcome this challenge, allowing for the measurement of scattered light in a lateral direction. In this work, we developed an optical adapter that allows for the rapid assessment of the optimal measurement angle of excitation and detection within these 3D-printed cultivation vessels. The findings of the present study indicate that an angle of 110° yields higher signal intensities and enhanced sensitivity in comparison to larger angles, and this finding was additionally confirmed for a set of biotechnologically relevant microorganisms. While these results suggest that the angle of 110° should be integrated into the cultivation vessels in the future, the optical adapter also holds the potential to further investigate vessels with different sizes or geometries.
KW - 3D printing
KW - Light scattering
KW - Online monitoring
KW - Optical waveguide
UR - http://www.scopus.com/inward/record.url?scp=105018345153&partnerID=8YFLogxK
U2 - 10.1007/s00216-025-06131-4
DO - 10.1007/s00216-025-06131-4
M3 - Article
C2 - 41039129
AN - SCOPUS:105018345153
VL - 417
SP - 5627
EP - 5635
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
SN - 1618-2642
IS - 25
ER -