Details
| Original language | English |
|---|---|
| Pages (from-to) | 1955-1965 |
| Number of pages | 11 |
| Journal | International Journal of Biological Macromolecules |
| Volume | 104 |
| Early online date | 30 Mar 2017 |
| Publication status | Published - Nov 2017 |
Abstract
The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce “ready-to-use” tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol. Biocompatibility of the macroporous, flat chitin scaffolds has been confirmed by cell attachment, high cell viability and the ability to differentiate into the adipogenic lineage. The viability of cells cryopreserved on chitin scaffolds was reduced by about 30% as compared to cells cryopreserved in suspension. However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage. The results suggest that chitin from the marine demosponge I. basta is a promising, highly biocompatible biomaterial for stem cell-based tissue-engineering applications.
Keywords
- Chitin, Marine sponges, Mesenchymal stromal cells, Scaffolds, Tissue engineering
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Structural Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Economics, Econometrics and Finance(all)
- Economics and Econometrics
- Energy(all)
- General Energy
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In: International Journal of Biological Macromolecules, Vol. 104, 11.2017, p. 1955-1965.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Novel chitin scaffolds derived from marine sponge Ianthella basta for tissue engineering approaches based on human mesenchymal stromal cells
T2 - Biocompatibility and cryopreservation
AU - Mutsenko, Vitalii V.
AU - Gryshkov, Oleksandr
AU - Lauterboeck, Lothar
AU - Rogulska, Olena
AU - Tarusin, Dmitriy N.
AU - Bazhenov, Vasilii V.
AU - Schütz, Kathleen
AU - Brüggemeier, Sophie
AU - Gossla, Elke
AU - Akkineni, Ashwini R.
AU - Meißner, Heike
AU - Lode, Anja
AU - Meschke, Stephan
AU - Fromont, Jane
AU - Stelling, Allison L.
AU - Tabachnik, Konstantin R.
AU - Gelinsky, Michael
AU - Nikulin, Sergey
AU - Rodin, Sergey
AU - Tonevitsky, Alexander G.
AU - Petrenko, Alexander Y.
AU - Glasmacher, Birgit
AU - Schupp, Peter J.
AU - Ehrlich, Hermann
N1 - Funding information: The present work was partially supported by Leonhard-Euler-Programm from the German Academic Exchange Service (DAAD, Germany), IP@Leibniz program of the Leibniz Universität Hannover promoted by the DAAD (Germany, project code 57156199) as well as DFG Grant EH 394-3 (DFG, Germany). The authors are very grateful to Igor Kovalenko, Vitaliy Kholodny, Bulat Sydykov, Galina Bozhok and Ortrud Zieschang for the excellent technical assistance.
PY - 2017/11
Y1 - 2017/11
N2 - The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce “ready-to-use” tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol. Biocompatibility of the macroporous, flat chitin scaffolds has been confirmed by cell attachment, high cell viability and the ability to differentiate into the adipogenic lineage. The viability of cells cryopreserved on chitin scaffolds was reduced by about 30% as compared to cells cryopreserved in suspension. However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage. The results suggest that chitin from the marine demosponge I. basta is a promising, highly biocompatible biomaterial for stem cell-based tissue-engineering applications.
AB - The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce “ready-to-use” tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol. Biocompatibility of the macroporous, flat chitin scaffolds has been confirmed by cell attachment, high cell viability and the ability to differentiate into the adipogenic lineage. The viability of cells cryopreserved on chitin scaffolds was reduced by about 30% as compared to cells cryopreserved in suspension. However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage. The results suggest that chitin from the marine demosponge I. basta is a promising, highly biocompatible biomaterial for stem cell-based tissue-engineering applications.
KW - Chitin
KW - Marine sponges
KW - Mesenchymal stromal cells
KW - Scaffolds
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85018779427&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2017.03.161
DO - 10.1016/j.ijbiomac.2017.03.161
M3 - Article
C2 - 28365291
AN - SCOPUS:85018779427
VL - 104
SP - 1955
EP - 1965
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
ER -