Details
| Original language | English |
|---|---|
| Pages (from-to) | 34-39 |
| Number of pages | 6 |
| Journal | Acta Biomaterialia |
| Volume | 10 |
| Issue number | 1 |
| Publication status | Published - 7 Sept 2014 |
Abstract
An appropriate pH level is an important prerequisite for the physiologal functioning of cells and tissues. Changes in the extracellular pH often lead to specific cellular reactions and an altered metabolism of cells and tissues influences the extracellular pH range. Thus a method to monitor the extracellular pH is a valuable tool to track specific tissue reactions. In this article we describe a method for the determination of the pH range adjacent to degradable biomaterials using wireless in vivo imaging. Using hairless but immunocompetent mice the fluorophor 5-(6)-carboxy SNARF-1 and the in vivo fluorescence and multispectral acquisition and analysis system Maestro it is possible to track shifts in pH in small living animals over a longer period of time. This method is especially suitable for studies which focus on the interaction of degrading biomaterials with their adjacent tissues.
Keywords
- Biocompatibility, Biodegradation, Image analysis, In vivo imaging, Magnesium
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Materials Science(all)
- Biomaterials
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Engineering(all)
- Biomedical Engineering
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: Acta Biomaterialia, Vol. 10, No. 1, 07.09.2014, p. 34-39.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Non-invasive pH determination adjacent to degradable biomaterials in vivo
AU - Bartsch, Ivonne
AU - Willbold, Elmar
AU - Rosenhahn, Bodo
AU - Witte, Frank
N1 - Funding information: The authors are grateful for the financial support of the International Bone Research Association. We also thank Heike Achilles, Maike Haupt and Mattias Reebmann from Hannover Medical School for helpful discussions and excellent technical support.
PY - 2014/9/7
Y1 - 2014/9/7
N2 - An appropriate pH level is an important prerequisite for the physiologal functioning of cells and tissues. Changes in the extracellular pH often lead to specific cellular reactions and an altered metabolism of cells and tissues influences the extracellular pH range. Thus a method to monitor the extracellular pH is a valuable tool to track specific tissue reactions. In this article we describe a method for the determination of the pH range adjacent to degradable biomaterials using wireless in vivo imaging. Using hairless but immunocompetent mice the fluorophor 5-(6)-carboxy SNARF-1 and the in vivo fluorescence and multispectral acquisition and analysis system Maestro it is possible to track shifts in pH in small living animals over a longer period of time. This method is especially suitable for studies which focus on the interaction of degrading biomaterials with their adjacent tissues.
AB - An appropriate pH level is an important prerequisite for the physiologal functioning of cells and tissues. Changes in the extracellular pH often lead to specific cellular reactions and an altered metabolism of cells and tissues influences the extracellular pH range. Thus a method to monitor the extracellular pH is a valuable tool to track specific tissue reactions. In this article we describe a method for the determination of the pH range adjacent to degradable biomaterials using wireless in vivo imaging. Using hairless but immunocompetent mice the fluorophor 5-(6)-carboxy SNARF-1 and the in vivo fluorescence and multispectral acquisition and analysis system Maestro it is possible to track shifts in pH in small living animals over a longer period of time. This method is especially suitable for studies which focus on the interaction of degrading biomaterials with their adjacent tissues.
KW - Biocompatibility
KW - Biodegradation
KW - Image analysis
KW - In vivo imaging
KW - Magnesium
UR - http://www.scopus.com/inward/record.url?scp=84888639270&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2013.08.047
DO - 10.1016/j.actbio.2013.08.047
M3 - Article
C2 - 24021229
AN - SCOPUS:84888639270
VL - 10
SP - 34
EP - 39
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
IS - 1
ER -