Non-invasive Methods for Determination of Cellular Growth in Podophyllum hexandrum Suspension Cultures

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Authors

  • Saurabh Chattopadhyay
  • V. S. Bisaria
  • T. Scheper
  • A. K. Srivastava

Research Organisations

External Research Organisations

  • Indian Institute of Technology Delhi (IITD)
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Details

Original languageEnglish
Pages (from-to)331-334
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Volume7
Issue number6
Publication statusPublished - 2002

Abstract

Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation in a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heteroge-nous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.

Keywords

    Conductivity measurement, NADH measurement, On-line biomass measurement, Podophyllum hexandrum

ASJC Scopus subject areas

Cite this

Non-invasive Methods for Determination of Cellular Growth in Podophyllum hexandrum Suspension Cultures. / Chattopadhyay, Saurabh; Bisaria, V. S.; Scheper, T. et al.
In: Biotechnology and Bioprocess Engineering, Vol. 7, No. 6, 2002, p. 331-334.

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T1 - Non-invasive Methods for Determination of Cellular Growth in Podophyllum hexandrum Suspension Cultures

AU - Chattopadhyay, Saurabh

AU - Bisaria, V. S.

AU - Scheper, T.

AU - Srivastava, A. K.

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